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Infect."],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The important role of plasmid genes in assessing virulence for BALB\/c mice in salmonella, and the difficulty of using standard techniques to detect them, led us to develop a detection method by gene amplification.<\/jats:p><jats:p>One hundred and forty\u2013three strains (71 serovars) of salmonella and 35 strains of other species were tested using specific oligonucleotide primers. The amplification products were identified by a specific oligonucleotide probe. Forty-nine salmonella strains from ten serovars (<jats:italic>S. abortus ovis, S. choleraesuis, S. dublin, S. enteritidis, S. gallinarum \/ pullorum, S. hessarek, S. typhimurium, S. IIIa<\/jats:italic>48:z<jats:sub>4<\/jats:sub>, z<jats:sub>23<\/jats:sub>,<jats:italic>S. IV<\/jats:italic>43: z<jats:sub>4<\/jats:sub>, z<jats:sub>23<\/jats:sub>:-,<jats:italic>S. V<\/jats:italic>28:a:-) produced a positive and specific response.<\/jats:p><jats:p>Because of various origins of the strains possessing the gene sought and the diversity of the responses, both from one serovar to another and in the same serovar, this search has its place among the epidemiological markers in general use. This method appears well suited to the research and detection of plasmid genes associated with mouse virulence in salmonella.<\/jats:p>","DOI":"10.1017\/s0950268800057393","type":"journal-article","created":{"date-parts":[[2009,5,15]],"date-time":"2009-05-15T08:56:51Z","timestamp":1242377811000},"page":"33-43","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":15,"title":["Polymerase chain reaction for salmonella virulence\u2013associated plasmid genes detection: a new tool in salmonella epidemiology"],"prefix":"10.1017","volume":"112","author":[{"given":"L.","family":"Rexach","sequence":"first","affiliation":[]},{"given":"F.","family":"Dilasser","sequence":"additional","affiliation":[]},{"given":"P.","family":"Fach","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2009,5,15]]},"reference":[{"key":"S0950268800057393_ref010","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/153.6.1119"},{"key":"S0950268800057393_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/S0769-2609(86)80093-X"},{"key":"S0950268800057393_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90081-8"},{"key":"S0950268800057393_ref020","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1128\/IAI.50.2.586-587.1985","article-title":"Plasmid-cured Salmonella enteritidis AL1192 as a candidate for a live vaccine","volume":"50","author":"Nakamura","year":"1985","journal-title":"Infect Immun"},{"key":"S0950268800057393_ref030","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.1.1"},{"key":"S0950268800057393_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/S0769-2609(84)80080-0"},{"key":"S0950268800057393_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90194-0"},{"key":"S0950268800057393_ref009","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1128\/IAI.41.1.443-444.1983","article-title":"Correlation between the presence of a 50 MDa plasmid in Salmonella dublin and virulence for mice","volume":"41","author":"Terakado","year":"1983","journal-title":"Infect Immun"},{"key":"S0950268800057393_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90066-1"},{"key":"S0950268800057393_ref031","doi-asserted-by":"publisher","DOI":"10.4267\/2042\/64388"},{"key":"S0950268800057393_ref021","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00222.x"},{"key":"S0950268800057393_ref014","first-page":"523","article-title":"Propri\u00e9t\u00e9s ph\u00e9notypiques et g\u00e9notypiques de 4 plasmides de virulence de Salmonella typhimurium","volume":"138","author":"Pohl","year":"1987","journal-title":"Ann Inst Pasteur Microbiol"},{"key":"S0950268800057393_ref026","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1128\/IAI.48.1.175-182.1985","article-title":"Epidemiology of virulence associated plasmids and other membrane protein patterns within 7 common Salmonella serotypes","volume":"48","author":"Helmuth","year":"1985","journal-title":"Infect Immun"},{"key":"S0950268800057393_ref015","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1991.tb00536.x"},{"key":"S0950268800057393_ref018","first-page":"503","article-title":"Distribution of virulence plasmids within Salmonella","volume":"135","author":"Woodward","year":"1989","journal-title":"J Gen Microbiol"},{"key":"S0950268800057393_ref008","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1991.tb05287.x"},{"key":"S0950268800057393_ref011","first-page":"3136","article-title":"Functional homology of virulence plasmid in Salmonella pullorum. 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