{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T05:56:42Z","timestamp":1772085402136,"version":"3.50.1"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2008,9,26]],"date-time":"2008-09-26T00:00:00Z","timestamp":1222387200000},"content-version":"unspecified","delay-in-days":4531,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zygote"],"published-print":{"date-parts":[[1996,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Adducin is a cytoskeletal protein that can function<jats:italic>in vitro<\/jats:italic>to bundle F-actin and to control the assembly of the F-actin\/spectrin cytoskeletal network. The<jats:italic>Drosophila Adducin-like<\/jats:italic>(<jats:italic>Add<\/jats:italic>) locus (also referred to as<jats:italic>hu-li tai shao<\/jats:italic>(<jats:italic>hts<\/jats:italic>)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding<jats:italic>et al., Proc. Natl. Acad. Sci. USA<\/jats:italic><jats:bold>90<\/jats:bold>, 2512\u201316, 1993; Yue &amp; Spradling,<jats:italic>Genes Dev.<\/jats:italic><jats:bold>6<\/jats:bold>, 2443\u201354, 1992). We report the identification of two novel adducin isoforms: a 95 \u00d7 10<jats:sup>3<\/jats:sup><jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>form (ADD-95) and an 87 \u00d7 10<jats:sup>3<\/jats:sup><jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>form (ADD-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissuetypes; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of<jats:italic>Add-hts<\/jats:italic>mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.<\/jats:p>","DOI":"10.1017\/s096719940000304x","type":"journal-article","created":{"date-parts":[[2008,9,26]],"date-time":"2008-09-26T14:46:08Z","timestamp":1222440368000},"page":"159-166","source":"Crossref","is-referenced-by-count":102,"title":["Differential distributions of two adducin-like protein isoforms in the<i>Drosophila<\/i>ovary and early embryo"],"prefix":"10.1017","volume":"4","author":[{"given":"Mich\u00e8le","family":"Zaccai","sequence":"first","affiliation":[]},{"given":"Howard D.","family":"Lipshitz","sequence":"additional","affiliation":[]}],"member":"56","published-online":{"date-parts":[[2008,9,26]]},"reference":[{"key":"S096719940000304X_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90005-4"},{"key":"S096719940000304X_ref016","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1242\/dev.120.7.2015","article-title":"Morphogenesis of Drosophila ovarian ring canals","volume":"120","author":"Robinson","year":"1994","journal-title":"Development"},{"key":"S096719940000304X_ref013","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1242\/dev.120.4.947","article-title":"The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation","volume":"120","author":"Lin","year":"1994","journal-title":"Development."},{"key":"S096719940000304X_ref012","volume-title":"Ovarian Development in Drosophila melanogaster","author":"King","year":"1970"},{"key":"S096719940000304X_ref011","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.557"},{"key":"S096719940000304X_ref009","volume-title":"Antibodies: A Laboratory Manual. Cold Spring Harbor","author":"Harlow","year":"1988"},{"key":"S096719940000304X_ref008","doi-asserted-by":"publisher","DOI":"10.1038\/328359a0"},{"key":"S096719940000304X_ref006","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2512"},{"key":"S096719940000304X_ref005","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950151004"},{"key":"S096719940000304X_ref001","volume-title":"Current Protocols in Molecular Biology","volume":"2","author":"Ausubel","year":"1994"},{"key":"S096719940000304X_ref020","doi-asserted-by":"publisher","DOI":"10.1101\/gad.6.12b.2443"},{"key":"S096719940000304X_ref014","first-page":"2847","article-title":"Erythrocyte adducin: a calmodulin-regulated actin-bunding protein that stimulates spectrin\u2013actin binding","volume":"105","author":"Mishe","year":"1987","journal-title":"J. Cell Biol."},{"key":"S096719940000304X_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90232-R"},{"key":"S096719940000304X_ref004","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-02454-6"},{"key":"S096719940000304X_ref007","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.5.2095"},{"key":"S096719940000304X_ref019","unstructured":"Yue L. (1992). Genetic and molecular characterization of huli tai shas (hts), a gene required for ring canal formation during Drosophila oogenesis. PhD thesis, Johns Hopkins University, Baltimore, Maryland."},{"key":"S096719940000304X_ref003","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.5.2103"},{"key":"S096719940000304X_ref002","doi-asserted-by":"crossref","first-page":"5860","DOI":"10.1016\/S0021-9258(18)60645-3","article-title":"Brain adducin: a protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction","volume":"263","author":"Bennett","year":"1988","journal-title":"J. Biol. Chem."},{"key":"S096719940000304X_ref010","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.3.665"},{"key":"S096719940000304X_ref018","first-page":"41","volume-title":"Localized RNAs","author":"Whittaker","year":"1995"}],"container-title":["Zygote"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S096719940000304X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T09:35:44Z","timestamp":1631871344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S096719940000304X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,5]]}},"alternative-id":["S096719940000304X"],"URL":"https:\/\/doi.org\/10.1017\/s096719940000304x","relation":{},"ISSN":["0967-1994","1469-8730"],"issn-type":[{"value":"0967-1994","type":"print"},{"value":"1469-8730","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5]]}}}