{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:19:25Z","timestamp":1787498365423,"version":"build-2736575974"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"unspecified","delay-in-days":170,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ASHE"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"S2732494X22002261_as1\">\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>\n                      Antimicrobial resistance (AMR) is a global priority with significant clinical and economic consequences. Multidrug-resistant (MDR)\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      is one of the major pathogens associated with significant morbidity and mortality. In healthcare settings, the evaluation of prevalence, microbiological characteristics, as well as mechanisms of resistance is of paramount importance to overcome associated challenges.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S2732494X22002261_as2\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Consecutive clinical specimens of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      were collected prospectively from 5 acute-care and specialized hospitals between October 2014 and September 2017, including microbiological, clinical characteristics and outcomes. Identification and antimicrobial susceptibility test were performed using the BD Phoenix identification and susceptibility testing system, matrix-assisted laser desorption ionization\u2013time-of-flight mass spectrometry (MALDI-TOF MS), and minimum inhibitory concentration (MIC) test strips. Overall, 78 selected MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolates were processed for whole-genome sequencing (WGS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S2732494X22002261_as3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The overall prevalence of MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolates was 5.9% (525 of 8,892) and showed a decreasing trend; 95% of cases were hospital acquired and 44.8% were from respiratory samples. MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      demonstrated &gt;86% resistance to cefepime, ciprofloxacin, meropenem, and piperacillin-tazobactam but 97.5% susceptibility to colistin. WGS revealed 29 different sequence types: 20.5% ST235, 10.3% ST357, 7.7% ST389, and 7.7% ST1284. ST233 was associated with bloodstream infections and increased 30-day mortality. All ST389 isolates were obtained from patients with cystic fibrosis. Encoded exotoxin genes were detected in 96.2% of isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S2732494X22002261_as4\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolated from clinical specimens from Qatar has significant resistance to most agents, with a decreasing trend that should be explored further. Genomic analysis revealed the dominance of 5 main clonal clusters associated with mortality and bloodstream infections. Microbiological and genomic monitoring of MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      has enhanced our understanding of AMR in Qatar.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1017\/ash.2022.226","type":"journal-article","created":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T04:18:55Z","timestamp":1655698735000},"source":"Crossref","is-referenced-by-count":18,"title":["Prevalence and microbiological and genetic characteristics of multidrug-resistant\n                    <i>Pseudomonas aeruginosa<\/i>\n                    over three years in Qatar"],"prefix":"10.1017","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6186-7770","authenticated-orcid":false,"given":"Mazen A.","family":"Sid Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8647-1695","authenticated-orcid":false,"given":"Hamad","family":"Abdel Hadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulieman","family":"Abu Jarir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Ahmad Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5994-4944","authenticated-orcid":false,"given":"Mohammed A.","family":"Arbab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jemal M.","family":"Hamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Alyazidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna A.","family":"Al-Maslamani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9125-1889","authenticated-orcid":false,"given":"Sini","family":"Skariah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali A.","family":"Sultan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Latif","family":"Al Khal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"S\u00f6derquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emad Bashir","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7957-0310","authenticated-orcid":false,"given":"Jana","family":"Jass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5562-7693","authenticated-orcid":false,"given":"Hisham","family":"Ziglam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"S2732494X22002261_ref17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"S2732494X22002261_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.infpip.2019.100027"},{"key":"S2732494X22002261_ref33","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10010042"},{"key":"S2732494X22002261_ref19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06094-11"},{"key":"S2732494X22002261_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(01)00267-3"},{"key":"S2732494X22002261_ref18","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"S2732494X22002261_ref10","unstructured":"10. 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