{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T21:14:05Z","timestamp":1762982045957,"version":"3.33.0"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"unspecified","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ASHE"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:sec id=\"S2732494X24004820_as1\">\n\t    <jats:title>Objective:<\/jats:title>\n\t    <jats:p>Discrepancies or inaccuracies between testing methods can create confusion or lead to clinical harm if antibiotics are inappropriately chosen. We report our clinical experience using the Accelerate Pheno\u2122 followed by routine automated susceptibilities by the Vitek\u00ae2 for positive blood cultures with gram-negative rods.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as2\">\n\t    <jats:title>Design:<\/jats:title>\n\t    <jats:p>This was a retrospective review of positive gram-negative blood cultures, including comparison of susceptibility testing results and impact on clinical care.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as4\">\n\t    <jats:title>Setting:<\/jats:title>\n\t    <jats:p>Academic teaching hospital.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as3\">\n\t    <jats:title>Patients (participants):<\/jats:title>\n\t    <jats:p>All patients admitted to the hospital with gram-negative bacteremia from January 2020 to December 2022.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as5\">\n\t    <jats:title>Methods:<\/jats:title>\n\t    <jats:p>Microbiology was reviewed for discrepancies as defined by very major errors (VMEs), major errors (MEs), and minor errors (mEs). Clinical charts were reviewed for antibiotic therapy.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as6\">\n\t    <jats:title>Results:<\/jats:title>\n\t    <jats:p>Positive blood cultures with gram-negative rods were included (n = 262). Between these two methods, overall essential agreement was 93.7% (2162\/2304) and categorical agreement 93.5% (2159\/2306). There were 147 discrepancies noted, including 6 VMEs, 25 MEs, and 116 mEs accounting for 96 patients. Antibiotic choice was changed in 8 patients due to perceived suboptimal empiric therapy based on the rapid susceptibility results.<\/jats:p>\n\t  <\/jats:sec>\n\t  <jats:sec id=\"S2732494X24004820_as7\">\n\t    <jats:title>Conclusions:<\/jats:title>\n\t    <jats:p>The Accelerate Pheno tended to over-call resistance compared to the Vitek\u00ae2. Few patients (8) received the incorrect antibiotic based on the Pheno result. Stewardship programs may choose to optimize their rapid antibiotic susceptibility testing reporting to help minimize confusion and guide appropriate antibiotic selection.<\/jats:p>\n\t  <\/jats:sec>","DOI":"10.1017\/ash.2024.482","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T07:55:58Z","timestamp":1737618958000},"source":"Crossref","is-referenced-by-count":1,"title":["Delving into discrepancies, a single-center experience with Accelerate Pheno for gram-negative bacteremia, a rapid phenotypic susceptibility testing method"],"prefix":"10.1017","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2931-3660","authenticated-orcid":false,"given":"Zoe","family":"Freeman Weiss","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitar","family":"Zelenkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Englert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5167-0766","authenticated-orcid":false,"given":"Maureen","family":"Campion","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,1,23]]},"reference":[{"key":"S2732494X24004820_ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3287-6"},{"key":"S2732494X24004820_ref11","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab126"},{"key":"S2732494X24004820_ref21","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01649-19"},{"key":"S2732494X24004820_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2020.07.015"},{"key":"S2732494X24004820_ref22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01672-17"},{"key":"S2732494X24004820_ref20","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00095-17"},{"key":"S2732494X24004820_ref8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa528"},{"key":"S2732494X24004820_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.03.038"},{"key":"S2732494X24004820_ref10","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab165"},{"key":"S2732494X24004820_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2014.05.005"},{"key":"S2732494X24004820_ref15","unstructured":"15. De Angelis, G , Posteraro, B , Menchinelli, G , Liotti, FM , Spanu, T , Sanguinetti, M. Antimicrobial susceptibility testing of pathogens isolated from blood culture: a performance comparison of Accelerate Pheno\u2122 and VITEK\u00ae 2 systems with the broth microdilution method."},{"key":"S2732494X24004820_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa649"},{"key":"S2732494X24004820_ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-021-01581-1"},{"key":"S2732494X24004820_ref12","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.635831"},{"key":"S2732494X24004820_ref16","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01329-17"},{"key":"S2732494X24004820_ref26","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04673-22"},{"key":"S2732494X24004820_ref2","doi-asserted-by":"publisher","DOI":"10.1592\/phco.29.11.1326"},{"key":"S2732494X24004820_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg234"},{"key":"S2732494X24004820_ref13","article-title":"Rapid versus standard antimicrobial susceptibility testing to guide treatment of bloodstream infection","volume":"5","author":"Anton-Vazquez","year":"2021","journal-title":"Cochrane Database Syst Rev"},{"key":"S2732494X24004820_ref1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11040427"},{"key":"S2732494X24004820_ref7","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac347"},{"key":"S2732494X24004820_ref23","first-page":"e01166","article-title":"Use of the accelerate pheno system for identification and antimicrobial susceptibility testing of pathogens in positive blood cultures and impact on time to results and workflow","volume":"56","author":"Charnot-Katsikas","year":"2017","journal-title":"J Clin Microbiol"},{"key":"S2732494X24004820_ref19","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02364-20"},{"key":"S2732494X24004820_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00122-22"},{"key":"S2732494X24004820_ref24","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky531"},{"key":"S2732494X24004820_ref3","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00181-17"}],"container-title":["Antimicrobial Stewardship &amp; Healthcare Epidemiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S2732494X24004820","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T07:56:01Z","timestamp":1737618961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S2732494X24004820\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025]]}},"alternative-id":["S2732494X24004820"],"URL":"https:\/\/doi.org\/10.1017\/ash.2024.482","relation":{},"ISSN":["2732-494X"],"issn-type":[{"value":"2732-494X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"article-number":"e15"}}