{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:47:21Z","timestamp":1791964041873,"version":"4.3.4"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Neurol. Sci."],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec id=\"S031716712610674X_as1\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Diagnosis of sporadic Creutzfeldt\u2013Jakob disease (sCJD) has traditionally relied on non-specific cerebrospinal fluid (CSF) biomarkers such as 14-3-3\u03b3 and total tau. The development of quaking-induced conversion (QuIC) assays has enabled direct detection of prion seeds, improving antemortem diagnostic accuracy. We previously noted a high CSF submission rate from the Canadian province of New Brunswick. We leveraged this observation to evaluate how expanded regional testing influences prion disease surveillance and the diagnostic performance of EP-QuIC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S031716712610674X_as2\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>CSF submissions between 2016 and 2024 from all provinces to the Public Health Agency of Canada\u2019s National Microbiology Laboratory were considered, and EP-QuIC results were analyzed in the context of biomarker profiles and patient demographics. Biochemical subtype distribution was examined to assess whether increased testing density facilitates the detection of subtypes with less common clinical presentations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S031716712610674X_as3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The CSF submissions provided an opportunity to assess EP-QuIC performance in a cohort with increased testing rates. EP-QuIC maintained high diagnostic reliability, demonstrating strong performance even when clinical suspicion was expected to be low. Additionally, a modest rise in VV2 cases was observed, suggesting that broader testing could improve the identification of those sCJD subtypes that may not initially meet criteria for classic CJD and could otherwise be underrecognized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S031716712610674X_as4\">\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Enhanced regional EP-QuIC testing supports more robust prion disease surveillance and retains high diagnostic accuracy across varied clinical contexts. Increased testing density may also improve detection of some sCJD subtypes, highlighting the value of increased access to EP-QuIC testing.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1017\/cjn.2026.10674","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:14:29Z","timestamp":1791962069000},"page":"1-8","source":"Crossref","is-referenced-by-count":0,"title":["Expanded EP-QuIC Testing for Sporadic CJD in a Local Geographic Area of Canada Results in Improved Surveillance"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6863-2643","authenticated-orcid":false,"given":"Ben A.","family":"Bailey-Elkin","sequence":"first","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9025-8368","authenticated-orcid":false,"given":"Jessy A.","family":"Slota","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Myskiw","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Avery","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clark","family":"Phillipson","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic M.S.","family":"Kielich","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Lamoureux","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Frost","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.R.","family":"Simon","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie L.","family":"Sim","sequence":"additional","affiliation":[{"name":"University of Alberta"},{"name":"University of Alberta"},{"name":"University of Alberta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-7666","authenticated-orcid":false,"given":"Gerard H.","family":"Jansen","sequence":"additional","affiliation":[{"name":"University of Ottawa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie A.","family":"Booth","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada"},{"name":"University of Manitoba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"S031716712610674X_ref8","doi-asserted-by":"publisher","DOI":"10.1002\/ana.78162"},{"key":"S031716712610674X_ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0585-1"},{"key":"S031716712610674X_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-017-1683-0"},{"key":"S031716712610674X_ref7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00542-16"},{"key":"S031716712610674X_ref31","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-021-00488-7"},{"key":"S031716712610674X_ref19","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14422"},{"key":"S031716712610674X_ref12","article-title":"Creutzfeldt-Jakob disease surveillance in Australia: update to 31 December 2023","volume":"49","author":"Collins","year":"2025","journal-title":"Commun Dis Intell"},{"key":"S031716712610674X_ref26","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2020-323231"},{"key":"S031716712610674X_ref27","doi-asserted-by":"publisher","DOI":"10.1002\/ana.78117"},{"key":"S031716712610674X_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/cjn.2025.10088"},{"key":"S031716712610674X_ref22","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.71.3.401"},{"key":"S031716712610674X_ref24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-63545-5"},{"key":"S031716712610674X_ref23","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-220718"},{"key":"S031716712610674X_ref14","unstructured":"14. 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