{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T09:11:51Z","timestamp":1778577111968,"version":"3.51.4"},"reference-count":59,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"unspecified","delay-in-days":250,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut. Microb."],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Microbial dysbiosis has been linked to environmental enteropathy (EE) and alterations in nutrient absorption; however, compositional modifications following exposure to supplementary nutrients are poorly understood. Here, we report the effect of amino acid and micronutrient supplementation on the gut microbiome of adults with EE.<\/jats:p>\n                  <jats:p>In the AMAZE trial, adults with EE were randomized to amino acids (AA) and\/or micronutrients (MM) for 16\u00a0weeks in a 2\u00a0\u00d7\u00a02 factorial design against placebo. Endoscopy was performed before and after intervention, during which duodenal aspirates were collected as well as fecal samples. 16S rRNA amplicon sequencing was performed on both these samples, and differences in bacterial community composition before and after interventions were investigated using differential abundance analysis, corrected using false discovery rate, plus alpha and beta diversity measurements.<\/jats:p>\n                  <jats:p>HIV seropositive participants exhibited lower alpha and beta diversity at baseline. AA and\/or MM supplementation did not show significant changes in abundance or diversity of genera post-intervention compared to placebo. Micronutrient supplementation resulted in an increase in the pyruvate fermentation to acetone MetaCyc pathways compared to the placebo arm.<\/jats:p>\n                  <jats:p>This study provides insights into the responsiveness of the gut microbiome to micronutrient and amino acid supplementation in adults with EE.<\/jats:p>","DOI":"10.1017\/gmb.2025.10011","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T09:59:12Z","timestamp":1757325552000},"source":"Crossref","is-referenced-by-count":0,"title":["Intestinal microbiome changes in response to amino acid and micronutrient supplementation: secondary analysis of the AMAZE trial"],"prefix":"10.1017","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2679-3697","authenticated-orcid":false,"given":"Monica N.","family":"Mweetwa","sequence":"first","affiliation":[{"name":"University of Zambia School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazi","family":"Ahsan","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Louis-Auguste","sequence":"additional","affiliation":[{"name":"University of Zambia School of Medicine"},{"name":"Queen Mary University of London"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Besa","sequence":"additional","affiliation":[{"name":"University of Zambia School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joram M.","family":"Posma","sequence":"additional","affiliation":[{"name":"Imperial College"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan P.","family":"McNulty","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Barratt","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey I.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Washington University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0844-6448","authenticated-orcid":false,"given":"Paul","family":"Kelly","sequence":"additional","affiliation":[{"name":"University of Zambia School of Medicine"},{"name":"Queen Mary University of London"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"S263228972510011X_r33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajcnut.2023.11.018"},{"key":"S263228972510011X_r42","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.646467"},{"key":"S263228972510011X_r39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0298592"},{"key":"S263228972510011X_r41","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty633"},{"key":"S263228972510011X_r13","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012352626117"},{"key":"S263228972510011X_r25","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.70.412"},{"key":"S263228972510011X_r53","doi-asserted-by":"publisher","DOI":"10.1038\/s41562-023-01725-3"},{"key":"S263228972510011X_r56","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-023-02880-0"},{"key":"S263228972510011X_r52","unstructured":"Wickham, H (2016) ggplot2:Elegant Graphics for Data Analysis. 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