{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:11:53Z","timestamp":1790179913526,"version":"4.1.0"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:00:00Z","timestamp":1498003200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infect. Control Hosp. Epidemiol."],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec id=\"S0899823X17001155_abs1\" sec-type=\"general\"><jats:title>OBJECTIVE<\/jats:title><jats:p>To compare different techniques of endoscope sampling to assess residual bacterial contamination.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X17001155_abs2\" sec-type=\"general\"><jats:title>DESIGN<\/jats:title><jats:p>Diagnostic study.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X17001155_abs3\" sec-type=\"general\"><jats:title>SETTING<\/jats:title><jats:p>The endoscopy unit of an 1,100-bed university hospital performing ~13,000 endoscopic procedures annually.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X17001155_abs4\" sec-type=\"methods\"><jats:title>METHODS<\/jats:title><jats:p>In total, 4 sampling techniques, combining flushing fluid with or without a commercial endoscope brush, were compared in an endoscope model. Based on these results, sterile physiological saline flushing with or without PULL THRU brush was selected for evaluation on 40 flexible endoscopes by adenosine triphosphate (ATP) measurement and bacterial culture. Acceptance criteria from the French National guideline (&lt;25 colony-forming units [CFU] per endoscope and absence of indicator microorganisms) were used as part of the evaluation.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X17001155_abs5\" sec-type=\"results\"><jats:title>RESULTS<\/jats:title><jats:p>On biofilm-coated PTFE tubes, physiological saline in combination with a PULL THRU brush generated higher mean ATP values (2,579 relative light units [RLU]) compared with saline alone (1,436 RLU; <jats:italic>P<\/jats:italic>=.047). In the endoscope samples, culture yield using saline plus the PULL THRU (mean, 43 CFU; range, 1\u2013400 CFU) was significantly higher than that of saline alone (mean, 17 CFU; range, 0\u2013500 CFU; <jats:italic>P<\/jats:italic>&lt;.001). In samples obtained using the saline+PULL THRU brush method, ATP values of samples classified as unacceptable were significantly higher than those of samples classified as acceptable (<jats:italic>P<\/jats:italic>=.001).<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X17001155_abs6\" sec-type=\"conclusion\"><jats:title>CONCLUSION<\/jats:title><jats:p>Physiological saline flushing combined with PULL THRU brush to sample endoscopes generated higher ATP values and increased the yield of microbial surveillance culture. Consequently, the acceptance rate of endoscopes based on a defined CFU limit was significantly lower when the saline+PULL THRU method was used instead of saline alone.<\/jats:p><jats:p><jats:italic>Infect Control Hosp Epidemiol<\/jats:italic> 2017;38:1062\u20131069<\/jats:p><\/jats:sec>","DOI":"10.1017\/ice.2017.115","type":"journal-article","created":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T07:57:08Z","timestamp":1498031828000},"page":"1062-1069","source":"Crossref","is-referenced-by-count":33,"title":["Surveillance of Endoscopes: Comparison of Different Sampling Techniques"],"prefix":"10.1017","volume":"38","author":[{"given":"Lien","family":"Cattoir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Vanzieleghem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Florin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Helleputte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"De Vos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Verhasselt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerina","family":"Boelens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Leroux-Roels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,6,21]]},"reference":[{"key":"S0899823X17001155_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2016.10.017"},{"key":"S0899823X17001155_ref15","unstructured":"Infection control in endoscopy. Gastroenterological Society of Australia website. http:\/\/cart.gesa.org.au\/membes\/files\/Clinical%20Guidelines%20and%20Updates\/Infection_Control_in_Endoscopy_Guidelines_2014.pdf. Published 2010. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2004.06.023"},{"key":"S0899823X17001155_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2011.03.1183"},{"key":"S0899823X17001155_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2012.12.008"},{"key":"S0899823X17001155_ref12","unstructured":"Comit\u00e9 Technique des Infections Nosocomiales et des Infections Li\u00e9es aux Soins (CTINILS). \u00c9l\u00e9ments d\u2019assurance qualit\u00e9 en hygi\u00e8ne relatifs au contr\u00f4le microbiologique des endoscopes et \u00e0 la tra\u00e7abilit\u00e9 en endoscopie. French Ministery of Health and Solidarity website. http:\/\/social-sante.gouv.fr\/IMG\/pdf\/microbio_endoscopes-2.pdf. Published 2007. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2014.01.044"},{"key":"S0899823X17001155_ref16","unstructured":"Manitoba Health. Guidelines for infection prevention and control in endoscopy. The College of Physicians and Surgeons of Manitoba, Canada, website. http:\/\/cpsm.mb.ca\/cjj39alckF30a\/wp-content\/uploads\/NHMSF%20Appendix%20F%20Endoscopy%20Guidelines.pdf. Published 2000. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref2","doi-asserted-by":"publisher","DOI":"10.1155\/2001\/691584"},{"key":"S0899823X17001155_ref21","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2015.212"},{"key":"S0899823X17001155_ref4","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-995477"},{"key":"S0899823X17001155_ref11","unstructured":"Stuurgroep flexibele endoscopen reiniging en desinfectie (SFERD). Professional standard handbook cleaning and disinfection flexible endoscopes, version 3.0. Dutch Sterilisation Society website. https:\/\/www.infectiepreventieopleidingen.nl\/downloads\/SFERDHandbook3_1.pdf. Published 2014. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2010.07.008"},{"key":"S0899823X17001155_ref5","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1055\/s-0042-107591","article-title":"Measures to improve microbial quality surveillance of gastrointestinal endoscopes","volume":"48","author":"Saliou","year":"2016","journal-title":"Endoscopy"},{"key":"S0899823X17001155_ref20","doi-asserted-by":"publisher","DOI":"10.5946\/ce.2015.48.5.369"},{"key":"S0899823X17001155_ref13","unstructured":"BSG Guidelines for decontamination of equipment for gastrointestinal endoscopy. British Society of Gastroenterology website. http:\/\/www.bsg.org.uk\/pdf_word_docs\/decontamination_2008.pdf. Published 2008. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2012.02.030"},{"key":"S0899823X17001155_ref14","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-945181"},{"key":"S0899823X17001155_ref1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00085-12"},{"key":"S0899823X17001155_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2007.05264.x"},{"key":"S0899823X17001155_ref10","unstructured":"Aanbevelingen inzake het onderhoud van flexibel warmtegevoelig endoscopisch materiaal en de preventie van infecties, 5 May 2010. Superior Health Council of Belgium website. https:\/\/www.health.belgium.be\/nl\/advies-8355-endoscopisch-materiaal. Published 2010. Accessed January 24, 2017."},{"key":"S0899823X17001155_ref18","doi-asserted-by":"publisher","DOI":"10.1067\/mic.2000.106054"},{"key":"S0899823X17001155_ref24","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06221-11"},{"key":"S0899823X17001155_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S1329-9360(16)30008-6"}],"container-title":["Infection Control &amp; Hospital Epidemiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0899823X17001155","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T01:36:28Z","timestamp":1555464988000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0899823X17001155\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,21]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["S0899823X17001155"],"URL":"https:\/\/doi.org\/10.1017\/ice.2017.115","relation":{},"ISSN":["0899-823X","1559-6834"],"issn-type":[{"value":"0899-823X","type":"print"},{"value":"1559-6834","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,21]]}}}