{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:46:52Z","timestamp":1789501612004,"version":"build-2803163510"},"reference-count":69,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T00:00:00Z","timestamp":1574380800000},"content-version":"unspecified","delay-in-days":325,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Nutr Sci"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study aimed to investigate whether dietary choline can regulate lipid metabolism and suppress NF\u03baB activation and, consequently, attenuate inflammation induced by a high-fat diet in black sea bream (<jats:italic>Acanthopagrus schlegelii<\/jats:italic>). An 8-week feeding trial was conducted on fish with an initial weight of 8\u00b716 \u00b1 0\u00b701 g. Five diets were formulated: control, low-fat diet (11 %); HFD, high-fat diet (17 %); and HFD supplemented with graded levels of choline (3, 6 or 12 g\/kg) termed HFD + C1, HFD + C2 and HFD + C3, respectively. Dietary choline decreased lipid content in whole body and tissues. Highest TAG and cholesterol concentrations in serum and liver were recorded in fish fed the HFD. Similarly, compared with fish fed the HFD, dietary choline reduced vacuolar fat drops and ameliorated HFD-induced pathological changes in liver. Expression of genes of lipolysis pathways were up-regulated, and genes of lipogenesis down-regulated, by dietary choline compared with fish fed the HFD. Expression of<jats:italic>nf\u03bab<\/jats:italic>and pro-inflammatory cytokines in liver and intestine was suppressed by choline supplementation, whereas expression of anti-inflammatory cytokines was promoted in fish fed choline-supplemented diets. In fish that received lipopolysaccharide to stimulate inflammatory responses, the expression of<jats:italic>nf\u03bab<\/jats:italic>and pro-inflammatory cytokines in liver, intestine and kidney were all down-regulated by dietary choline compared with the HFD. Overall, the present study indicated that dietary choline had a lipid-lowering effect, which could protect the liver by regulating intrahepatic lipid metabolism, reducing lipid droplet accumulation and suppressing NF\u03baB activation, consequently attenuating HFD-induced inflammation in<jats:italic>A. schlegelii<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/jns.2019.34","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T15:22:27Z","timestamp":1574436147000},"source":"Crossref","is-referenced-by-count":98,"title":["Dietary choline supplementation attenuated high-fat diet-induced inflammation through regulation of lipid metabolism and suppression of NF\u03baB activation in juvenile black seabream (<i>Acanthopagrus schlegelii<\/i>)"],"prefix":"10.1017","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-0292","authenticated-orcid":false,"given":"Min","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas R.","family":"Tocher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica 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