{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:44:31Z","timestamp":1790847871328,"version":"4.1.0"},"reference-count":58,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Acta Neuropsychiatr."],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0924270823000145_as2\"><jats:title>Objective<\/jats:title><jats:p>Cocaine is a highly addictive psychostimulant that affects synaptic activity with structural and functional adaptations of neurons. The transmembrane synaptic vesicle glycoprotein 2A (SV2A) of pre-synaptic vesicles is commonly used to measure synaptic density, as a novel approach to the detection of synaptic changes. We do not know if a single dose of cocaine suffices to affect pre-synaptic SV2A density, especially during adolescence when synapses undergo intense maturation. Here, we explored potential changes of pre-synaptic SV2A density in target brain areas associated with the cocaine-induced boost of dopaminergic neurotransmission, specifically testing if the effects would last after the return of dopamine levels to baseline.<\/jats:p><\/jats:sec><jats:sec id=\"S0924270823000145_as3\"><jats:title>Methods:<\/jats:title><jats:p>We administered cocaine (20 mg\/kg i.p.) or saline to rats in early adolescence, tested their activity levels and removed the brains 1 hour and 7 days after injection. To evaluate immediate and lasting effects, we did autoradiography with [<jats:sup>3<\/jats:sup>H]UCB-J, a specific tracer for SV2A, in medial prefrontal cortex, striatum, nucleus accumbens, amygdala, and dorsal and ventral areas of hippocampus. We also measured the striatal binding of [<jats:sup>3<\/jats:sup>H]GBR-12935 to test cocaine\u2019s occupancy of the dopamine transporter at both times of study.<\/jats:p><\/jats:sec><jats:sec id=\"S0924270823000145_as4\"><jats:title>Results:<\/jats:title><jats:p>We found a significant increase of [<jats:sup>3<\/jats:sup>H]UCB-J binding in the dorsal and ventral sections of hippocampus 7 days after the cocaine administration compared to saline-injected rats, but no differences 1 hour after the injection. The [<jats:sup>3<\/jats:sup>H]GBR-12935 binding remained unchanged at both times.<\/jats:p><\/jats:sec><jats:sec id=\"S0924270823000145_as5\"><jats:title>Conclusion:<\/jats:title><jats:p>Cocaine provoked lasting changes of hippocampal synaptic SV2A density after a single exposure during adolescence<\/jats:p><\/jats:sec>","DOI":"10.1017\/neu.2023.14","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T11:58:01Z","timestamp":1677499081000},"page":"109-117","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["A single dose of cocaine raises SV2A density in hippocampus of adolescent rats"],"prefix":"10.1017","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1050-1966","authenticated-orcid":false,"given":"Rachele","family":"Rossi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1935-3650","authenticated-orcid":false,"given":"Simone Larsen","family":"B\u00e6rentzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2914-9576","authenticated-orcid":false,"given":"Majken B.","family":"Thomsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-3341","authenticated-orcid":false,"given":"Caroline C.","family":"Real","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0081-0068","authenticated-orcid":false,"given":"Gregers","family":"Wegener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9911-5921","authenticated-orcid":false,"given":"Rodrigo","family":"Grassi-Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3756-7401","authenticated-orcid":false,"given":"Albert","family":"Gjedde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7371-8713","authenticated-orcid":false,"given":"Anne M.","family":"Landau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2023,2,27]]},"reference":[{"key":"S0924270823000145_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2018.08.006"},{"key":"S0924270823000145_ref6","doi-asserted-by":"publisher","DOI":"10.1113\/JP282601"},{"key":"S0924270823000145_ref55","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1301194"},{"key":"S0924270823000145_ref23","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890030107"},{"key":"S0924270823000145_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2019.02.033"},{"key":"S0924270823000145_ref45","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9121876"},{"key":"S0924270823000145_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15523"},{"key":"S0924270823000145_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-014-3708-x"},{"key":"S0924270823000145_ref31","doi-asserted-by":"publisher","DOI":"10.1101\/lm.042192.116"},{"key":"S0924270823000145_ref39","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-022-01342-0"},{"key":"S0924270823000145_ref24","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.2510140408"},{"key":"S0924270823000145_ref49","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20931140"},{"key":"S0924270823000145_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8636-6"},{"key":"S0924270823000145_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2021.105002"},{"key":"S0924270823000145_ref16","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.1918"},{"key":"S0924270823000145_ref57","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(91)90154-T"},{"key":"S0924270823000145_ref50","doi-asserted-by":"publisher","DOI":"10.1038\/35079077"},{"key":"S0924270823000145_ref40","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020835"},{"key":"S0924270823000145_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2021.03.002"},{"key":"S0924270823000145_ref44","first-page":"624","article-title":"The laboratory rat: relating its age with human\u2019s","volume":"4","author":"Sengupta","year":"2013","journal-title":"International Journal of Preventive Medicine"},{"key":"S0924270823000145_ref3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2150"},{"key":"S0924270823000145_ref28","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.22250"},{"key":"S0924270823000145_ref33","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3498"},{"key":"S0924270823000145_ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-017-4559-z"},{"key":"S0924270823000145_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.07.001"},{"key":"S0924270823000145_ref22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03712.x"},{"key":"S0924270823000145_ref58","doi-asserted-by":"publisher","DOI":"10.2174\/187152708786927877"},{"key":"S0924270823000145_ref27","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12345"},{"key":"S0924270823000145_ref32","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201300482"},{"key":"S0924270823000145_ref47","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.05.001"},{"key":"S0924270823000145_ref52","doi-asserted-by":"publisher","DOI":"10.1002\/syn.22157"},{"key":"S0924270823000145_ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2005.04538.x"},{"key":"S0924270823000145_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2014.07.004"},{"key":"S0924270823000145_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(83)90038-3"},{"key":"S0924270823000145_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2020.108161"},{"key":"S0924270823000145_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.dcn.2020.100857"},{"key":"S0924270823000145_ref19","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00349.2006"},{"key":"S0924270823000145_ref41","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00576.x"},{"key":"S0924270823000145_ref43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05689.x"},{"key":"S0924270823000145_ref15","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099020"},{"key":"S0924270823000145_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113741"},{"key":"S0924270823000145_ref13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15268"},{"key":"S0924270823000145_ref56","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60732-2"},{"key":"S0924270823000145_ref54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2006133117"},{"key":"S0924270823000145_ref37","volume-title":"The rat brain in stereotaxic coordinates","author":"Paxinos","year":"2004"},{"key":"S0924270823000145_ref8","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.4.1284"},{"key":"S0924270823000145_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00274-3"},{"key":"S0924270823000145_ref17","doi-asserted-by":"publisher","DOI":"10.1109\/TNS.2008.2005673"},{"key":"S0924270823000145_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2016.03.062"},{"key":"S0924270823000145_ref42","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.864514"},{"key":"S0924270823000145_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(02)00059-X"},{"key":"S0924270823000145_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385506-0.00009-0"},{"key":"S0924270823000145_ref34","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.115.168179"},{"key":"S0924270823000145_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.13123"},{"key":"S0924270823000145_ref26","doi-asserted-by":"publisher","DOI":"10.1080\/10550490601184142"},{"key":"S0924270823000145_ref2","doi-asserted-by":"publisher","DOI":"10.1002\/dev.20252"},{"key":"S0924270823000145_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2005.03.005"},{"key":"S0924270823000145_ref53","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050358"}],"container-title":["Acta Neuropsychiatrica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0924270823000145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T10:14:27Z","timestamp":1709892867000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0924270823000145\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,27]]},"references-count":58,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S0924270823000145"],"URL":"https:\/\/doi.org\/10.1017\/neu.2023.14","relation":{},"ISSN":["0924-2708","1601-5215"],"issn-type":[{"value":"0924-2708","type":"print"},{"value":"1601-5215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,27]]},"assertion":[{"value":"\u00a9 The Author(s), 2023. Published by Cambridge University Press on behalf of Scandinavian College of Neuropsychopharmacology","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}