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We hypothesised that folic acid depletion<jats:italic>in utero<\/jats:italic>and during early neonatal life may affect tumorigenesis in offspring. To investigate this hypothesis, female C57Bl6\/J mice were randomised to a folic acid adequate (2\u00a0mg folic acid\/kg diet) or folic acid depleted diet (0\u00b74\u00a0mg folic acid\/kg) from mating with<jats:italic>Apc<\/jats:italic><jats:sup><jats:italic>+\/Min<\/jats:italic><\/jats:sup>sires and throughout pregnancy and lactation. At weaning the<jats:italic>Apc<\/jats:italic><jats:sup><jats:italic>+\/Min<\/jats:italic><\/jats:sup>offspring were randomised to a folic acid adequate (2\u00a0mg folic acid\/kg diet) or depleted (0\u00b726\u00a0mg folic acid\/kg diet) diet, creating four<jats:italic>in utero<\/jats:italic>\/post-weaning dietary regimens. At 10 weeks post-weaning, mice were killed and the intestinal tumour number and size were recorded. Folic acid depletion during pregnancy and post-weaning reduced erythrocyte folate concentrations in offspring significantly. Folic acid depletion during pregnancy and lactation did not affect tumour multiplicity or size. However, female mice fed normal folic acid diets post-weaning had more, and larger, tumours when compared with depleted females and both depleted and adequate folic acid fed males. These data suggest that folate depletion post-weaning was protective against neoplasia in female<jats:italic>Apc<\/jats:italic><jats:sup><jats:italic>+\/Min<\/jats:italic><\/jats:sup>mice and highlights the need for further investigation of the optimal timing and dose of folic acid supplementation with regard to colorectal cancer risk.<\/jats:p>","DOI":"10.1017\/s0007114507819131","type":"journal-article","created":{"date-parts":[[2007,9,14]],"date-time":"2007-09-14T14:45:47Z","timestamp":1189781147000},"page":"550-558","source":"Crossref","is-referenced-by-count":21,"title":["Gender-specific modulation of tumorigenesis by folic acid supply in the<i>Apc<\/i><sup><i>+\/Min<\/i><\/sup>mouse during early neonatal life"],"prefix":"10.1017","volume":"99","author":[{"given":"Jill A.","family":"McKay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth 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