{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:45:04Z","timestamp":1760982304496},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"S1","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>This trial was designed to assess the effect of live probiotic consumption on leucine assimilation from fresh and pasteurised yoghurt in volunteers with different lactose digestibility. Thirty-three volunteers (mean age 32, s.d. 7 years) participated in this parallel single-blind study (16 of them with moderate lactose intolerance). Breath samples were taken before and at 15\u00a0min intervals over 3-h after the ingestion of fresh and pasteurised yoghurt extrinsically labelled with (1-<jats:sup>13<\/jats:sup>C)leucine. The <jats:sup>13<\/jats:sup>C enrichment in breath was measured by isotopic rate mass spectrometry and mathematically converted to a percentage of assimilated leucine (100-%<jats:sup>13<\/jats:sup>C-dose in breath) and the assimilation kinetic constant (min<jats:sup>\u2212\u00a01<\/jats:sup>). The <jats:sup>13<\/jats:sup>C-leucine assimilation was statistically higher after the fresh yoghurt intake than after the pasteurised product intake (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7032) while the kinetic constant of assimilation was slower in intolerance status (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7014) although a product-related effect (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7445) was not found. In conclusion, fresh yoghurt intake resulted in higher short-term leucine assimilation, while lactose intolerance appears to negatively affect the assimilation rate of leucine from dairy products. These findings offer new insight on acute <jats:italic>in vivo<\/jats:italic> amino acid assimilation in the presence of probiotics and moderate lactose intolerance.<\/jats:p>","DOI":"10.1017\/s0007114507833058","type":"journal-article","created":{"date-parts":[[2007,10,9]],"date-time":"2007-10-09T09:33:37Z","timestamp":1191922417000},"page":"S101-S104","source":"Crossref","is-referenced-by-count":10,"title":["Amino acid uptake from a probiotic milk in lactose intolerant subjects"],"prefix":"10.1017","volume":"98","author":[{"given":"Dolores","family":"Parra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Alfredo Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,10,1]]},"reference":[{"key":"S0007114507833058_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-005-1101-6"},{"key":"S0007114507833058_ref16","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s003940070015","article-title":"Characterisation with stable isotopes of the presence of a lag phase in the gastric emptying of liquids","volume":"39","author":"Gonzalez","year":"2000","journal-title":"Eur J Nutr"},{"key":"S0007114507833058_ref14","doi-asserted-by":"publisher","DOI":"10.1007\/PL00014264"},{"key":"S0007114507833058_ref9","doi-asserted-by":"publisher","DOI":"10.3109\/07853899009147239"},{"key":"S0007114507833058_ref8","first-page":"A303","article-title":"Lactose and gluten intolerance: which to suscept?","volume":"21","author":"Van Gossum","year":"2000","journal-title":"Rev Med Brux"},{"key":"S0007114507833058_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2006.tb00218.x"},{"key":"S0007114507833058_ref6","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/72.6.1474"},{"key":"S0007114507833058_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8223(98)00152-7"},{"key":"S0007114507833058_ref4","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.0301207"},{"key":"S0007114507833058_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03168444"},{"key":"S0007114507833058_ref1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1601169"},{"key":"S0007114507833058_ref3","first-page":"208","article-title":"Monocyte function in healthy middle-aged people receiving fermented milk containing Lactobacillus casei","volume":"8","author":"Parra","year":"2004","journal-title":"J Nutr Health Aging"},{"key":"S0007114507833058_ref13","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.4.806"},{"key":"S0007114507833058_ref17","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2007.10719613"},{"key":"S0007114507833058_ref11","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002586"},{"key":"S0007114507833058_ref12","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/76.6.1326"},{"key":"S0007114507833058_ref15","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/127.2.327"},{"key":"S0007114507833058_ref10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2001.00952.x"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114507833058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T10:37:28Z","timestamp":1554979048000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114507833058\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":18,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["S0007114507833058"],"URL":"https:\/\/doi.org\/10.1017\/s0007114507833058","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}