{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:48:28Z","timestamp":1791398908843,"version":"4.3.3"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T00:00:00Z","timestamp":1223337600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2009,5,14]]},"abstract":"<jats:p>Nucleotide excision repair (NER) is responsible for repairing bulky helix-distorting DNA lesions and is essential for the maintenance of genomic integrity. Severe hereditary impairment of NER leads to cancers such as those in xeroderma pigmentosum, and more moderate reductions in NER capacity have been associated with an increased cancer risk. Diet is a proven modifier of cancer risk but few studies have investigated the potential relationships between diet and NER. In the present study, the plasmid-based host cell reactivation assay was used to measure the NER capacity in peripheral blood mononuclear cells from fifty-seven volunteers aged 18\u201330 years before and after 6 weeks of supplementation with micronutrients (selenium and vitamins A, C and E). As a control, nine individuals remained unsupplemented over the same period. Volunteers were genotyped for the following polymorphisms in NER genes: <jats:italic>ERCC5<\/jats:italic> Asp1104His (rs17655); <jats:italic>XPC<\/jats:italic> Lys939Gln (rs2228001); <jats:italic>ERCC2<\/jats:italic> Lys751Gnl (rs13181); <jats:italic>XPC<\/jats:italic> PAT (an 83\u00a0bp poly A\/T insertion\u2013deletion polymorphism in the <jats:italic>XPC<\/jats:italic> gene). NER capacity varied 11-fold between individuals and was inversely associated with age and endogenous DNA strand breaks. For the first time, we observed an inverse association between adiposity and NER. No single polymorphism was associated with the NER capacity, although significant gene\u2013gene interactions were observed between <jats:italic>XPC<\/jats:italic> Lys939Gln and <jats:italic>ERCC5<\/jats:italic> Asp1104His and <jats:italic>XPC<\/jats:italic> Lys939Gln and <jats:italic>ERCC2<\/jats:italic> Lys751Gnl. While there was no detectable effect of micronutrient supplementation on NER capacity, there was evidence that the effect of fruit intake on the NER capacity may be modulated by the <jats:italic>ERCC2<\/jats:italic> Lys751Gnl single nucleotide polymorphism.<\/jats:p>","DOI":"10.1017\/s0007114508076265","type":"journal-article","created":{"date-parts":[[2008,10,7]],"date-time":"2008-10-07T11:22:28Z","timestamp":1223378548000},"page":"1316-1323","source":"Crossref","is-referenced-by-count":51,"title":["Inter-individual variation in nucleotide excision repair in young adults: effects of age, adiposity, micronutrient supplementation and genotype"],"prefix":"10.1017","volume":"101","author":[{"given":"John","family":"Tyson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Caple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Spiers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Burtle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann K.","family":"Daly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Hesketh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Mathers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,10,7]]},"reference":[{"key":"S0007114508076265_manual_ref-32","doi-asserted-by":"publisher","DOI":"10.1038\/nature05456"},{"key":"S0007114508076265_manual_ref-16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-277X.2005.00593.x"},{"key":"S0007114508076265_manual_ref-13","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20031001"},{"key":"S0007114508076265_manual_ref-7","doi-asserted-by":"publisher","DOI":"10.2174\/156800907781386588"},{"key":"S0007114508076265_manual_ref-11","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/24.3.511"},{"key":"S0007114508076265_manual_ref-36","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.7.2281-2291.2001"},{"key":"S0007114508076265_manual_ref-3","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/23.2.295"},{"key":"S0007114508076265_manual_ref-29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.4.1614"},{"key":"S0007114508076265_manual_ref-33","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2004406"},{"key":"S0007114508076265_manual_ref-24","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.8.1527"},{"key":"S0007114508076265_manual_ref-18","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg120"},{"key":"S0007114508076265_manual_ref-19","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgh058"},{"key":"S0007114508076265_manual_ref-12","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0694fje"},{"key":"S0007114508076265_manual_ref-4","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.08.019"},{"key":"S0007114508076265_manual_ref-17","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(02)00219-1"},{"key":"S0007114508076265_manual_ref-25","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11346"},{"key":"S0007114508076265_manual_ref-27","doi-asserted-by":"publisher","DOI":"10.1038\/35077232"},{"key":"S0007114508076265_manual_ref-14","doi-asserted-by":"publisher","DOI":"10.1079\/NRR200132"},{"key":"S0007114508076265_manual_ref-28","doi-asserted-by":"publisher","DOI":"10.1067\/S0190-9622(03)00918-6"},{"key":"S0007114508076265_manual_ref-1","doi-asserted-by":"publisher","DOI":"10.1038\/35094000"},{"key":"S0007114508076265_manual_ref-26","article-title":"Comet assay-based methods for measuring DNA repair in vitro; 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