{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T22:59:08Z","timestamp":1788562748368,"version":"build-2803163510"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T00:00:00Z","timestamp":1229299200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"abstract":"<jats:p>Cu is an essential cofactor for at least twelve mammalian enzymes including dopamine \u03b2-mono-oxygenase (DBM), which converts dopamine (DA) to noradrenaline (NA). Previous studies reported that certain Cu-deficient (Cu\u2212) rat tissues have lower NA and higher DA than Cu-adequate (Cu+) tissues, suggesting that DBM function was impaired. However,<jats:italic>in vitro<\/jats:italic>studies suggested that DBM activity is higher in Cu\u2212 tissue. Experiments were conducted on adrenal glands (AG), medulla oblongata\/pons (MO), vas deferens (VD) and heart (HT) from a single rat experiment to provide data to help clarify this puzzling contradiction.<jats:italic>In vitro<\/jats:italic>DBM activity assays showed Cu\u2212 samples had significantly higher activity than Cu+ samples in both AG and MO, but not VD. Activity data were confirmed by Western immunoblots. Quantitative real-time PCR demonstrated higher DBM mRNA in Cu\u2212 tissues but unaltered levels of several other cuproenzymes and Cu-binding proteins. Previous pharmacological data implied that high DBM was associated with low NA. HPLC analyses confirmed that NA and DA levels in Cu\u2212 MO, VD and HT were significantly lower and higher, respectively, than in Cu+ tissues. However, the NA content of AG was not statistically lower. Furthermore there was no correlation between higher DBM mRNA and lower NA in four Cu\u2212tissues. Adequate dietary Cu is essential to support DBM function<jats:italic>in vivo<\/jats:italic>but additional studies are needed to determine the mechanism for increased DBM transcription associated with Cu deficiency.<\/jats:p>","DOI":"10.1017\/s0007114508162961","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T11:09:37Z","timestamp":1229339377000},"page":"18-28","source":"Crossref","is-referenced-by-count":15,"title":["Copper deficiency in rodents alters dopamine \u03b2-mono-oxygenase activity, mRNA and protein level"],"prefix":"10.1017","volume":"102","author":[{"given":"Kyle T.","family":"Nelson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph R.","family":"Prohaska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,12,15]]},"reference":[{"key":"S0007114508162961_ref35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1976.tb01495.x"},{"key":"S0007114508162961_ref7","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0031-6997(25)06912-1","article-title":"Dopamine-\u03b2-hydroxylase: regulation of its synthesis and release from nerve terminals","volume":"24","author":"Axelrod","year":"1972","journal-title":"Pharmacol Rev"},{"key":"S0007114508162961_ref40","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.18.1.1"},{"key":"S0007114508162961_ref34","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/113.8.1583"},{"key":"S0007114508162961_ref16","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/112.9.1706"},{"key":"S0007114508162961_ref11","doi-asserted-by":"publisher","DOI":"10.1038\/249852a0"},{"key":"S0007114508162961_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(91)90238-R"},{"key":"S0007114508162961_ref5","first-page":"458","volume-title":"Present Knowledge in Nutrition","author":"Prohaska","year":"2006"},{"key":"S0007114508162961_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2944(74)90035-0"},{"key":"S0007114508162961_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(03)00364-3"},{"key":"S0007114508162961_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(77)90252-0"},{"key":"S0007114508162961_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.12.2147"},{"key":"S0007114508162961_ref27","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.1999.11747268"},{"key":"S0007114508162961_ref21","first-page":"275","article-title":"Rodent brain and heart catecholamine levels are altered by different models of copper deficiency","volume":"145","author":"Pyatskowit","year":"2007","journal-title":"Comp Biochem Physiol C"},{"key":"S0007114508162961_ref4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1542\/peds.50.2.188","article-title":"Menkes's kinky hair syndrome. An inherited defect in copper absorption with widespread effects","volume":"50","author":"Danks","year":"1972","journal-title":"Pediatrics"},{"key":"S0007114508162961_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90586-9"},{"key":"S0007114508162961_ref3","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/93.4.533"},{"key":"S0007114508162961_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4492(77)90082-X"},{"key":"S0007114508162961_ref1","first-page":"1","volume-title":"Neurobiology of the Trace Elements","author":"Smith","year":"1983"},{"key":"S0007114508162961_ref2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1987.67.3.858"},{"key":"S0007114508162961_ref6","doi-asserted-by":"crossref","first-page":"4763","DOI":"10.1016\/S0021-9258(18)97021-3","article-title":"3,4-Dihydroxyphenylethylamine \u03b2-hydroxylase. Physical properties, copper content, and role of copper in the catalytic activity","volume":"240","author":"Friedman","year":"1965","journal-title":"J Biol Chem"},{"key":"S0007114508162961_ref8","doi-asserted-by":"publisher","DOI":"10.1038\/374643a0"},{"key":"S0007114508162961_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(67)90058-1"},{"key":"S0007114508162961_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1974.tb06920.x"},{"key":"S0007114508162961_ref13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.63041551.x"},{"key":"S0007114508162961_ref14","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.7.1226"},{"key":"S0007114508162961_ref17","doi-asserted-by":"publisher","DOI":"10.1177\/153537020122600307"},{"key":"S0007114508162961_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/0742-8413(90)90159-7"},{"key":"S0007114508162961_ref19","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490250317"},{"key":"S0007114508162961_ref24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm234"},{"key":"S0007114508162961_ref25","doi-asserted-by":"publisher","DOI":"10.1177\/153537020422900807"},{"key":"S0007114508162961_ref26","doi-asserted-by":"publisher","DOI":"10.1080\/10284150500097182"},{"key":"S0007114508162961_ref28","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.8.2253"},{"key":"S0007114508162961_ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb10092.x"},{"key":"S0007114508162961_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(81)90068-9"},{"key":"S0007114508162961_ref32","doi-asserted-by":"publisher","DOI":"10.1042\/bst0080342"},{"key":"S0007114508162961_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/0955-2863(90)90015-D"},{"key":"S0007114508162961_ref39","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa070613"},{"key":"S0007114508162961_ref20","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S0022-3565(25)30199-0","article-title":"Effects of reserpine on activities and amounts of tyrosine hydroxylase and dopamine-\u03b2-hydroxylase in catecholamine neuronal sytems in rat brain","volume":"193","author":"Reis","year":"1975","journal-title":"J Pharmacol Exp Ther"},{"key":"S0007114508162961_ref38","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0065-3101(24)00096-3","article-title":"Menkes disease","volume":"41","author":"Kaler","year":"1994","journal-title":"Adv Pediatr"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114508162961","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T05:22:46Z","timestamp":1738819366000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114508162961\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,15]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,7,14]]}},"alternative-id":["S0007114508162961"],"URL":"https:\/\/doi.org\/10.1017\/s0007114508162961","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,15]]}}}