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Recent studies by us have shown that vitamin C deficiency may result in impaired brain development. Thus, the aim of the present study was to investigate if a poor diet high in fat and cholesterol affects the vitamin C status of guinea pigs kept on either sufficient or deficient levels of dietary ascorbate (Asc) for up to 6 months with particular emphasis on the brain. The present results show that a high-fat and cholesterol diet significantly decreased the vitamin C concentrations in the brain, irrespective of the vitamin C status of the animal (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b7001). The brain Asc oxidation ratio only depended on vitamin C status (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001) and not on the dietary lipid content. In plasma, the levels of Asc significantly decreased when vitamin C in the diet was low or when the fat\/cholesterol content was high (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001 for both). The Asc oxidation ratio increased both with low vitamin C and with high fat and cholesterol content (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001 for both). We show here for the first time that vitamin C homoeostasis of brain is affected by a diet rich in fat and cholesterol. The present findings suggest that this type of diet increases the turnover of Asc; hence, individuals consuming high-lipid diets may be at increased risk of vitamin C deficiency.<\/jats:p>","DOI":"10.1017\/s0007114510003077","type":"journal-article","created":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T09:29:52Z","timestamp":1285666192000},"page":"54-61","source":"Crossref","is-referenced-by-count":34,"title":["High dietary fat and cholesterol exacerbates chronic vitamin C deficiency in guinea pigs"],"prefix":"10.1017","volume":"105","author":[{"given":"Henriette","family":"Frikke-Schmidt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pernille","family":"Tveden-Nyborg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malene Muusfeldt","family":"Birck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Lykkesfeldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2010,9,28]]},"reference":[{"key":"S0007114510003077_ref43","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21625"},{"key":"S0007114510003077_ref63","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/65.4.959"},{"key":"S0007114510003077_ref69","first-page":"2","article-title":"Diet and tobacco use: analysis of data from the diabetic control and complications trial, a randomized study","volume":"4","author":"Cundiff","year":"2002","journal-title":"MedGenMed"},{"key":"S0007114510003077_ref2","first-page":"417","article-title":"Chronic vitamin C deficiency increases the risk of cardiovascular diseases","volume":"108","author":"Ginter","year":"2007","journal-title":"Bratisl Lek Listy"},{"key":"S0007114510003077_ref67","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-32232-9_9"},{"key":"S0007114510003077_ref62","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/71.2.530"},{"key":"S0007114510003077_ref60","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/54.6.1113s"},{"key":"S0007114510003077_ref58","doi-asserted-by":"publisher","DOI":"10.1007\/BF02535748"},{"key":"S0007114510003077_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)01091-2"},{"key":"S0007114510003077_ref47","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2006.10719538"},{"key":"S0007114510003077_ref45","doi-asserted-by":"publisher","DOI":"10.1186\/1743-7075-3-17"},{"key":"S0007114510003077_ref41","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116491"},{"key":"S0007114510003077_ref37","first-page":"49","article-title":"Aggravation of cholesterol induced hyperlipidemia by chronic vitamin C deficiency: experimental study in guinea pigs","volume":"39","author":"Kothari","year":"1988","journal-title":"Acta Biol Hung"},{"key":"S0007114510003077_ref39","first-page":"65","article-title":"The effect of ascorbic acid on the lipid metabolism of guinea-pigs fed an atherogenic diet","volume":"11","author":"Ginter","year":"1969","journal-title":"Cor Vasa"},{"key":"S0007114510003077_ref34","first-page":"307","article-title":"Effect of vitamin C deficiency and excess on the liver: a histopathological and biochemical study in guinea pigs fed normal or high cholesterol diet","volume":"33","author":"Sharma","year":"1990","journal-title":"Indian J Pathol Microbiol"},{"key":"S0007114510003077_ref49","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665109990279"},{"key":"S0007114510003077_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04128-3"},{"key":"S0007114510003077_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80356-H"},{"key":"S0007114510003077_ref27","first-page":"7.6.1","volume-title":"Current Protocols in Toxicology","author":"Lykkesfeldt","year":"2002"},{"key":"S0007114510003077_ref26","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4592"},{"key":"S0007114510003077_ref23","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.1.10"},{"key":"S0007114510003077_ref22","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.36.227"},{"key":"S0007114510003077_ref5","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.23.3116"},{"key":"S0007114510003077_ref20","doi-asserted-by":"crossref","first-page":"10764","DOI":"10.1016\/S0021-9258(18)90577-6","article-title":"Carnitine biosynthesis from gamma-butyrobetaine and from exogenous protein-bound 6-N-trimethyl-l-lysine by the perfused guinea pig liver. 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