{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:07:55Z","timestamp":1790892475265,"version":"4.1.0"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2010,11,9]],"date-time":"2010-11-09T00:00:00Z","timestamp":1289260800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2011,3,28]]},"abstract":"<jats:p>Production and manufacturing methods and the food carrier may influence the properties of probiotic strains, and have an impact on the outcome of clinical intervention studies. The aim of the present study was to establish whether the properties of a specific probiotic strain,<jats:italic>Lactobacillus rhamnosus<\/jats:italic>GG, may differ depending on the product and source of the strain. In total, fifteen different<jats:italic>L. rhamnosus<\/jats:italic>isolates, among them fourteen labelled as<jats:italic>L.<\/jats:italic><jats:italic>rhamnosus<\/jats:italic>GG, were isolated from specific probiotic products. The micro-organisms were phenotypically and genotypically characterised. Their adhesion properties were compared using the human intestinal mucus model, and the ability of the isolates to influence model pathogen adhesion to human colonic mucus was assessed. All<jats:italic>L. rhamnosus<\/jats:italic>isolates used were confirmed as members of the species<jats:italic>L. rhamnosus<\/jats:italic>. Except the reference strain OL, all<jats:italic>L.<\/jats:italic><jats:italic>rhamnosus<\/jats:italic>isolates showed randomly amplified polymorphic DNA, enterobacterial repetitive intergenic consensus and pulsed-field gel electrophoresis profiles identical to that of<jats:italic>L. rhamnosus<\/jats:italic>GG (ATCC 53103). All<jats:italic>L.<\/jats:italic><jats:italic>rhamnosus<\/jats:italic>isolates showed similar tolerance to acid and were able to bind to human colonic mucus. However, pathogen exclusion by inhibition and competition varied significantly among the different<jats:italic>L. rhamnosus<\/jats:italic>isolates and pathogens tested. The results suggest that different sources of the same probiotic may have significantly altered strain properties. This should be considered in<jats:italic>in vivo<\/jats:italic>studies on human subjects and also for quality control of probiotic products.<\/jats:p>","DOI":"10.1017\/s0007114510004496","type":"journal-article","created":{"date-parts":[[2010,11,9]],"date-time":"2010-11-09T10:15:02Z","timestamp":1289297702000},"page":"887-894","source":"Crossref","is-referenced-by-count":108,"title":["Manufacturing process influences properties of probiotic bacteria"],"prefix":"10.1017","volume":"105","author":[{"given":"\u0141ukasz","family":"Grze\u015bkowiak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Isolauri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seppo","family":"Salminen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Gueimonde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2010,11,9]]},"reference":[{"key":"S0007114510004496_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-0144-8"},{"key":"S0007114510004496_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2004.04.006"},{"key":"S0007114510004496_ref24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04306.x"},{"key":"S0007114510004496_ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1991.tb00595.x"},{"key":"S0007114510004496_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00440-2"},{"key":"S0007114510004496_ref9","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1097\/MPG.0b013e31816e219e","article-title":"European Society for Paediatric Gastroenterology, Hepatology, and Nutrition\/European Society for Paediatric Infectious Diseases evidence-based guidelines for the management of acute gastroenteritis in children in Europe: executive summary","volume":"46","author":"Guarino","year":"2008","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"S0007114510004496_ref18","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1128\/.61.2.136-169.1997","article-title":"Common themes in microbial pathogenicity revisited","volume":"61","author":"Finlay","year":"1997","journal-title":"Microbiol Mol Biol Rev"},{"key":"S0007114510004496_ref17","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.324.7350.1361"},{"key":"S0007114510004496_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.08.036"},{"key":"S0007114510004496_ref19","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28522-0"},{"key":"S0007114510004496_ref10","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e31816b903f"},{"key":"S0007114510004496_ref3","first-page":"184","article-title":"Resected human colonic tissue: new model for characterizing adhesion of lactic acid bacteria","volume":"9","author":"Ouwehand","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"S0007114510004496_ref13","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.7.4296-4301.2003"},{"key":"S0007114510004496_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2006.11.007"},{"key":"S0007114510004496_ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2007.02212.x"},{"key":"S0007114510004496_ref5","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1093\/jn\/128.12.2313","article-title":"Probiotics reinforce mucosal degradation of antigens in rats: implications for therapeutic use of probiotics","volume":"128","author":"Pessi","year":"1998","journal-title":"J Nutr"},{"key":"S0007114510004496_ref8","first-page":"29","article-title":"Exogenous lactobacilli fed to man \u2013 their fate and ability to prevent diarrheal disease","volume":"7","author":"Clements","year":"1983","journal-title":"Prog Food Nutr Sci"},{"key":"S0007114510004496_ref1","volume-title":"Guidelines for the Evaluation of Probiotics in Food. 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