{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:07:01Z","timestamp":1791050821663,"version":"4.1.0"},"reference-count":56,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2011,1,31]],"date-time":"2011-01-31T00:00:00Z","timestamp":1296432000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2011,6,14]]},"abstract":"<jats:p>There is increasing interest in the potential chronic beneficial effects of dietary<jats:italic>n-<\/jats:italic>3 PUFA on the metabolic syndrome (MetS) and associated cardiovascular complications. We have recently established that increased dietary<jats:italic>n-<\/jats:italic>3 PUFA has a profound acute benefit on fasting lipids and the postprandial pro-inflammatory response in the JCR:LA-<jats:italic>cp<\/jats:italic>rat, a model of the MetS. However, it is unclear to what extent chronic dietary<jats:italic>n-<\/jats:italic>3 PUFA intervention can modulate the progression of end-stage metabolic and vascular complications. The present study aimed to determine the chronic effects of dietary<jats:italic>n-<\/jats:italic>3 PUFA supplementation on fasting and non-fasting dyslipidaemia, insulin resistance and vascular complications in the JCR:LA-<jats:italic>cp<\/jats:italic>rodent model. JCR:LA-<jats:italic>cp<\/jats:italic>rats were fed an isoenergetic lipid-balanced diet supplemented with 5\u00a0%<jats:italic>n-<\/jats:italic>3 PUFA (w\/w) of the total fat (fish oil-derived EPA\/DHA) for 16 weeks. Fasting and non-fasting (postprandial) plasma lipid profile was assessed. Hepatic and adipose tissue was probed for the expression of lipogenic proteins (acyl-CoA carboxylase (ACC), fatty acid synthase (FAS) and sterol regulatory element-binding protein-1 (SREBP-1)), while the activity of Jun N-terminal kinase (JNK) was assessed via Western blot to target phosphorylated JNK protein in primary enterocytes. The frequency of myocardial lesions was assessed by haematoxylin and eosin staining. Increased dietary<jats:italic>n-<\/jats:italic>3 PUFA improved both the fasting and postprandial lipid profiles (TAG, cholesterol and apoB48) in the JCR:LA-<jats:italic>cp<\/jats:italic>rat, potentially via the down-regulation of the hepatic or adipose tissue expression of lipogenic enzymes (ACC, FAS and SREBP-1). Rats fed the 5\u00a0%<jats:italic>n-<\/jats:italic>3 PUFA diet had lower (58\u00b72\u00a0%;<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701) enterocytic phosphorylated JNK protein and secreted less cholesterol (30\u00a0%;<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705) into mesenteric lymph compared with the control. The chronic metabolic benefits of dietary<jats:italic>n-<\/jats:italic>3 PUFA may underlie the potential to reduce vascular complications during the MetS, including the observed reduction in the frequency (approximately 80\u00a0%) of late-stage 3 myocardial lesions.<\/jats:p>","DOI":"10.1017\/s0007114510005453","type":"journal-article","created":{"date-parts":[[2011,1,31]],"date-time":"2011-01-31T11:10:11Z","timestamp":1296472211000},"page":"1572-1582","source":"Crossref","is-referenced-by-count":50,"title":["Chronic dietary<i>n<\/i>-3 PUFA intervention improves dyslipidaemia and subsequent cardiovascular complications in the JCR:LA-<i>cp<\/i>rat model of the metabolic syndrome"],"prefix":"10.1017","volume":"105","author":[{"given":"Jing","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faye","family":"Borthwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Hassanali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabban","family":"Mangat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Ruth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danni","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Jaeschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Russell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine J.","family":"Field","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spencer D.","family":"Proctor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna F.","family":"Vine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2011,1,31]]},"reference":[{"key":"S0007114510005453_ref51","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200002000-00002"},{"key":"S0007114510005453_ref45","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105711200"},{"key":"S0007114510005453_ref15","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1093\/ajcn\/85.5.1222","article-title":"Effects of fish-oil supplementation on myocardial fatty acids in humans","volume":"85","author":"Metcalf","year":"2007","journal-title":"Am J Clin Nutr"},{"key":"S0007114510005453_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosissup.2008.05.007"},{"key":"S0007114510005453_ref24","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1016\/S0021-9258(19)44141-0","article-title":"Intestinal epithelial cell surface membrane glycoprotein synthesis","volume":"248","author":"Weiser","year":"1973","journal-title":"J Biol Chem"},{"key":"S0007114510005453_ref21","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1016\/S0022-2275(20)42620-3","article-title":"Hypersecretion of VLDL, but not HDL, by hepatocyes from the JCR:LA-corpulent rat","volume":"31","author":"Vance","year":"1990","journal-title":"J Lipid Res"},{"key":"S0007114510005453_ref29","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1093\/ajcn\/70.5.817","article-title":"Dietary fish as a major component of a weight-loss diet: effect on serum lipids, glucose, and insulin metabolism in overweight hypertensive subjects","volume":"70","author":"Mori","year":"1999","journal-title":"Am J Clin Nutr"},{"key":"S0007114510005453_ref47","doi-asserted-by":"publisher","DOI":"10.1017\/S000711450999167X"},{"key":"S0007114510005453_ref48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4318"},{"key":"S0007114510005453_ref54","doi-asserted-by":"publisher","DOI":"10.1080\/07853890600852880"},{"key":"S0007114510005453_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2007.08.001"},{"key":"S0007114510005453_ref50","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.46.32725"},{"key":"S0007114510005453_ref13","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1093\/ajcn\/87.6.1991S","article-title":"Fish and n-3 fatty acids for the prevention of fatal coronary heart disease and sudden cardiac death","volume":"87","author":"Mozaffarian","year":"2008","journal-title":"Am J Clin Nutr"},{"key":"S0007114510005453_ref26","doi-asserted-by":"publisher","DOI":"10.1177\/000456329703400210"},{"key":"S0007114510005453_ref17","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.9.6.869"},{"key":"S0007114510005453_ref2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000111245.75752.C6"},{"key":"S0007114510005453_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2006.03.013"},{"key":"S0007114510005453_ref27","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.10.4.658"},{"key":"S0007114510005453_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2005.12.022"},{"key":"S0007114510005453_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.08.019"},{"key":"S0007114510005453_ref31","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72375-X"},{"key":"S0007114510005453_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2005.08.002"},{"key":"S0007114510005453_ref5","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.15.7.918"},{"key":"S0007114510005453_ref12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.91502.2007"},{"key":"S0007114510005453_ref9","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1093\/ajcn\/83.6.1499S","article-title":"n-3 Fatty acids and the metabolic syndrome","volume":"6","author":"Carpentier","year":"2006","journal-title":"Am J Clin Nutr"},{"key":"S0007114510005453_ref52","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve071"},{"key":"S0007114510005453_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2006.01858.x"},{"key":"S0007114510005453_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(94)90192-9"},{"key":"S0007114510005453_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosissup.2008.05.004"},{"key":"S0007114510005453_ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-199408000-00006"},{"key":"S0007114510005453_ref55","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-002-0874-6"},{"key":"S0007114510005453_ref28","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0803643"},{"key":"S0007114510005453_ref20","doi-asserted-by":"publisher","DOI":"10.1042\/BST0350477"},{"key":"S0007114510005453_ref7","doi-asserted-by":"publisher","DOI":"10.1046\/j.1463-1326.2002.00164.x"},{"key":"S0007114510005453_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-001-0768-3"},{"key":"S0007114510005453_ref49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25892"},{"key":"S0007114510005453_ref56","doi-asserted-by":"publisher","DOI":"10.3945\/jn.108.091009"},{"key":"S0007114510005453_ref43","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i21.3081"},{"key":"S0007114510005453_ref40","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000099786.99623.EF"},{"key":"S0007114510005453_ref23","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.109488"},{"key":"S0007114510005453_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-007-3039-3"},{"key":"S0007114510005453_ref46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.096107"},{"key":"S0007114510005453_ref38","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-006-1156-z"},{"key":"S0007114510005453_ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2009.01130.x"},{"key":"S0007114510005453_ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb51842.x"},{"key":"S0007114510005453_ref32","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002742"},{"key":"S0007114510005453_ref34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00414.2001"},{"key":"S0007114510005453_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(91)90008-L"},{"key":"S0007114510005453_ref35","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a117257"},{"key":"S0007114510005453_ref44","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0933"},{"key":"S0007114510005453_ref19","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1016\/S0022-2275(20)31533-9","article-title":"Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR:LA-cp rat","volume":"42","author":"Elam","year":"2001","journal-title":"J Lipid Res"},{"key":"S0007114510005453_ref6","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006828724405"},{"key":"S0007114510005453_ref8","first-page":"734","article-title":"Omega-3 fatty acids: their beneficial role in cardiovascular health","volume":"52","author":"Schwalfenberg","year":"2006","journal-title":"Can Fam Physician"},{"key":"S0007114510005453_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2005.12.024"},{"key":"S0007114510005453_ref53","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.cell.2010.07.041","article-title":"GPR120 is an omega-3 fatty acid receptor mediating potent anti-inflammatory and insulin-sensitizing effects","volume":"142","author":"Oh da","year":"2010","journal-title":"Cell"},{"key":"S0007114510005453_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2006.12.002"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114510005453","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T08:34:12Z","timestamp":1637224452000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114510005453\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,31]]},"references-count":56,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,6,14]]}},"alternative-id":["S0007114510005453"],"URL":"https:\/\/doi.org\/10.1017\/s0007114510005453","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,31]]}}}