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In Expt 1, <jats:italic>in situ<\/jats:italic> ligated duodenal loops from Mn-deficient chicks (29-d-old) were perfused with solutions containing 0\u20138\u00b774\u00a0mmol Mn\/l from either MnSO<jats:sub>4<\/jats:sub>, or one of two organic chelates of Mn and amino acids with moderate (OM) or strong (OS) chelation strength (<jats:italic>Q<\/jats:italic><jats:sub>f<\/jats:sub>) up to 30\u00a0min. In Expt 2, Mn-deficient intact broilers (14-d-old) were fed a control diet (12\u00b745\u00a0mg Mn\/kg) or the control diet supplemented with 100\u00a0mg Mn\/kg as one of all Mn sources for 14\u00a0d. The uptake kinetics of Mn from different Mn sources in the ligated duodenal loops followed a saturable process as determined by regression analysis of concentration-dependent uptake rates. The maximum transport rate (<jats:italic>J<\/jats:italic><jats:sub>max<\/jats:sub>) and <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values, and DMT1 mRNA levels in the ligated duodenal loops were higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701) for OM and OS than for MnSO<jats:sub>4<\/jats:sub>. DMT1 mRNA levels were much higher (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701) in the duodenum than in the jejunum and ileum. Both DMT1 mRNA levels in the duodenum and plasma Mn contents from the hepatic portal vein of intact chicks on day 14 post-feeding increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705) in the following order: control\u00a0&lt;\u00a0MnSO<jats:sub>4<\/jats:sub>\u00a0&lt;\u00a0OM\u00a0&lt;\u00a0OS. These results indicated that organic Mn sources with stronger <jats:italic>Q<\/jats:italic><jats:sub>f<\/jats:sub> showed higher Mn transport and absorption, and DMT1 might be involved in the regulation of organic Mn transport in the proximal small intestine of broilers.<\/jats:p>","DOI":"10.1017\/s0007114511005629","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T14:46:10Z","timestamp":1323960370000},"page":"267-276","source":"Crossref","is-referenced-by-count":51,"title":["Manganese source affects manganese transport and gene expression of divalent metal transporter 1 in the small intestine of broilers"],"prefix":"10.1017","volume":"108","author":[{"given":"Shi-Ping","family":"Bai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui-Lian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Xi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Yang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu-Gang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2011,12,15]]},"reference":[{"key":"S0007114511005629_ref45","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl017"},{"key":"S0007114511005629_ref11","doi-asserted-by":"publisher","DOI":"10.1038\/41343"},{"key":"S0007114511005629_ref27","volume-title":"SAS User's Guide: Statistics","year":"2003"},{"key":"S0007114511005629_ref19","volume-title":"Nutrient Requirements of Poultry","year":"1994"},{"key":"S0007114511005629_ref33","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106875"},{"key":"S0007114511005629_ref6","doi-asserted-by":"publisher","DOI":"10.2527\/2004.8282352x"},{"key":"S0007114511005629_ref30","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2008-1212"},{"key":"S0007114511005629_ref32","first-page":"32","volume-title":"The Roles of Amino Acid Chelates in Animal Nutrition","author":"Ashmead","year":"1993"},{"key":"S0007114511005629_ref44","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2009-1846"},{"key":"S0007114511005629_ref23","doi-asserted-by":"publisher","DOI":"10.1136\/gut.7.2.159"},{"key":"S0007114511005629_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.04.046"},{"key":"S0007114511005629_ref15","first-page":"12","article-title":"Chelation effectiveness of zinc proteinates demonstrated","volume":"67","author":"Holwerda","year":"1995","journal-title":"Feedstuffs"},{"key":"S0007114511005629_ref3","doi-asserted-by":"publisher","DOI":"10.1021\/bi801856k"},{"key":"S0007114511005629_ref28","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0680107"},{"key":"S0007114511005629_ref38","doi-asserted-by":"publisher","DOI":"10.1136\/gut.46.2.270"},{"key":"S0007114511005629_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0271-5317(89)80035-1"},{"key":"S0007114511005629_ref14","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.126680"},{"key":"S0007114511005629_ref39","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.105866"},{"key":"S0007114511005629_ref26","doi-asserted-by":"publisher","DOI":"10.22237\/jmasm\/1036110180"},{"key":"S0007114511005629_ref1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1981.61.2.249"},{"key":"S0007114511005629_ref2","first-page":"213","article-title":"Nutritional aspects of manganese from experimental studies","volume":"20","author":"Keen","year":"1999","journal-title":"Neurotoxicology"},{"key":"S0007114511005629_ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06145.x"},{"key":"S0007114511005629_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2005.12.005"},{"key":"S0007114511005629_ref8","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.7.1217"},{"key":"S0007114511005629_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s003600050085"},{"key":"S0007114511005629_ref12","doi-asserted-by":"publisher","DOI":"10.1021\/bi048768+"},{"key":"S0007114511005629_ref13","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00369"},{"key":"S0007114511005629_ref17","first-page":"313","article-title":"A study on the optimal manganese level in a practical diet of broiler chicks","volume":"22","author":"Luo","year":"1991","journal-title":"Clin J Anim Vet Sci"},{"key":"S0007114511005629_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.7.1217"},{"key":"S0007114511005629_ref20","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/106.7.958"},{"key":"S0007114511005629_ref22","unstructured":"22 Zhang TY (2002) Technique of phytase evaluation by digestion in vitro. 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