{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:43:16Z","timestamp":1792802596753,"version":"4.5.0"},"reference-count":48,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T00:00:00Z","timestamp":1333411200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2013,1,14]]},"abstract":"<jats:p>The present study aimed to explore the role(s) of the soya isoflavone genistein (GEN) in preventing the development of colon pre-neoplasia, using Wingless\/int (WNT)\/\u03b2-catenin as a molecular marker of colon abnormality. Specifically, the effects on the WNT\/\u03b2-catenin signalling pathway from GEN were examined by using an azoxymethane (AOM)-induced rat colon cancer model. Male Sprague\u2013Dawley rats were fed a control (CTL), a soya protein isolate (SPI) or a GEN diet from gestation to 13 weeks of age. The first sampling was conducted at 7 weeks of age for pre-AOM analysis. The remaining rats were injected with AOM at 7 weeks of age. The descending colon was collected 6 weeks later for the evaluation of aberrant crypt foci (ACF), gene expression and nuclear protein accumulation. AOM injection induced aberrant nuclear accumulation of \u03b2-catenin in the CTL group but not in the SPI or GEN group. Moreover, the WNT target genes<jats:italic>Cyclin D1<\/jats:italic>and<jats:italic>c-Myc<\/jats:italic>were repressed by SPI and GEN. Meanwhile, SPI and GEN suppressed the expression of WNT signalling genes including<jats:italic>Wnt5a<\/jats:italic>,<jats:italic>Sfrp1<\/jats:italic>,<jats:italic>Sfrp2<\/jats:italic>and<jats:italic>Sfrp5<\/jats:italic>to the similar level to that of the pre-AOM period. Rats fed SPI and GEN had a decreased number of total aberrant crypts. GEN feeding also resulted in a reduced number of ACF with<jats:italic>N<\/jats:italic>\u00a0=\u00a03 per foci. The reduction of WNT\/\u03b2-catenin signalling was correlated with the decrease in total aberrant crypts. By testing WNT\/\u03b2-catenin signalling as a biomarker of colon carcinogenic potential, we showed the novel role of GEN as a suppressor of carcinogen-induced WNT\/\u03b2-catenin signalling in preventing the development of early colon neoplasia.<\/jats:p>","DOI":"10.1017\/s0007114512000876","type":"journal-article","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T14:58:49Z","timestamp":1333465129000},"page":"33-42","source":"Crossref","is-referenced-by-count":78,"title":["Genistein, a soya isoflavone, prevents azoxymethane-induced up-regulation of WNT\/\u03b2-catenin signalling and reduces colon pre-neoplasia in 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