{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T08:09:38Z","timestamp":1793347778234,"version":"4.5.0"},"reference-count":47,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T00:00:00Z","timestamp":1373241600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2014,1,14]]},"abstract":"<jats:p>The present study was carried out to determine whether<jats:italic>N<\/jats:italic>-acetylcysteine (NAC) could modulate liver injury in a lipopolysaccharide (LPS)-challenged piglet model. For this purpose, eighteen piglets were randomly assigned to the control, LPS or NAC group. Piglets in the control and LPS groups were fed a basal diet, whereas those in the NAC group were fed the basal diet supplemented with 500\u00a0mg\/kg NAC. On days 10, 13 and 20 of the trial, the LPS- and NAC-treated piglets were intraperitoneally administered LPS (100\u00a0\u03bcg\/kg body weight), while the control group was administered the same volume of saline. On day 20 of the trial, blood samples were obtained 3\u00a0h after LPS or saline injection. On day 21, the piglets were killed to collect liver samples. Dietary NAC supplementation attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, in the LPS-challenged piglets, the activities of alanine aminotransferase and aspartate aminotransferase and the concentrations of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, TNF-\u03b1, IL-6 and PGE<jats:sub>2<\/jats:sub>were dramatically increased in the plasma and the activity of superoxide dismutase in the plasma and that of glutathione peroxidase in the liver were significantly decreased. The LPS challenge also increased the concentration of AMP and the ratio of AMP:ATP, but decreased adenylate energy charges and the levels of ATP and ADP. These adverse effects of the LPS challenge were ameliorated by NAC supplementation. Moreover, NAC inhibited the LPS-induced increases in the abundance of liver heat shock protein 70 and NF-\u03baB proteins. In conclusion, these results suggest that dietary NAC supplementation alleviates LPS-induced liver injury by reducing the secretion of pro-inflammatory cytokines, increasing the antioxidative capacity and improving energy metabolism.<\/jats:p>","DOI":"10.1017\/s0007114513002171","type":"journal-article","created":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T07:43:56Z","timestamp":1373269436000},"page":"46-54","source":"Crossref","is-referenced-by-count":55,"title":["Dietary<i>N<\/i>-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets"],"prefix":"10.1017","volume":"111","author":[{"given":"Dan","family":"Yi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqing","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binying","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengguo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhui","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2013,7,8]]},"reference":[{"key":"S0007114513002171_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0473-y"},{"key":"S0007114513002171_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(11)80342-6"},{"key":"S0007114513002171_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2009.11.007"},{"key":"S0007114513002171_ref3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00550.2001"},{"key":"S0007114513002171_ref23","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000153407.14237.7F"},{"key":"S0007114513002171_ref39","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199910000-00007"},{"key":"S0007114513002171_ref11","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000107562.68920.92"},{"key":"S0007114513002171_ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-012-1221-2"},{"key":"S0007114513002171_ref47","doi-asserted-by":"crossref","first-page":"16330","DOI":"10.1016\/S0021-9258(17)46227-2","article-title":"The oxidative inactivation of mitochondrial electron transport chain components and ATPase","volume":"265","author":"Zhang","year":"1990","journal-title":"J Biol Chem"},{"key":"S0007114513002171_ref18","unstructured":"Henneberg W & Stohmann F (1864) Beitr\u00e4ge zur Begr\u00fcndung einer rationellen F\u00fctterung der Wiederk\u00e4uer, (Contributions to Establish a Rational Feeding of Ruminants), 2nd ed., pp. 342. Braunschweig: Schwetschtke u. Sohn."},{"key":"S0007114513002171_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80454-5"},{"key":"S0007114513002171_ref14","doi-asserted-by":"crossref","unstructured":"Hou YQ, Wang L, Yi D, et al. (2012) N-Acetylcysteine reduces inflammation in the small intestine by regulating redox, EGF and TLR4 signaling. Amino Acids (epublication ahead of print version 25 April 2012).","DOI":"10.1007\/s00726-012-1295-x"},{"key":"S0007114513002171_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2003.12.001"},{"key":"S0007114513002171_ref37","doi-asserted-by":"publisher","DOI":"10.1007\/s000180300001"},{"key":"S0007114513002171_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.01.013"},{"key":"S0007114513002171_ref8","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.4049\/jimmunol.143.4.1290","article-title":"Reactive oxygen species facilitate the in vitro and in vivo lipopolysaccharide-induced release of tumor necrosis factor","volume":"143","author":"Chaudhri","year":"1989","journal-title":"J Immunol"},{"key":"S0007114513002171_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90112-4"},{"key":"S0007114513002171_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(98)90075-0"},{"key":"S0007114513002171_ref25","doi-asserted-by":"publisher","DOI":"10.1177\/1753425912441955"},{"key":"S0007114513002171_ref6","first-page":"1304","article-title":"Cytokine gene expression by Kupffer cells in experimental alcoholic liver disease","volume":"22","author":"Kamimura","year":"1995","journal-title":"Hepatology"},{"key":"S0007114513002171_ref12","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1152\/physrev.1994.74.1.139","article-title":"Cellular defenses against damage from reactive oxygen species","volume":"74","author":"Yu","year":"1994","journal-title":"Physiol Rev"},{"key":"S0007114513002171_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0740-y"},{"key":"S0007114513002171_ref32","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840190229"},{"key":"S0007114513002171_ref2","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1093\/jn\/131.5.1560","article-title":"Green tea suppresses lipopolysaccharide-induced liver injury in d-galactosamine sensitized rats","volume":"131","author":"He","year":"2001","journal-title":"J Nutr"},{"key":"S0007114513002171_ref4","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20282"},{"key":"S0007114513002171_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-011-1191-9"},{"key":"S0007114513002171_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.01.005"},{"key":"S0007114513002171_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-009-0269-0"},{"key":"S0007114513002171_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-009-0243-x"},{"key":"S0007114513002171_ref41","doi-asserted-by":"publisher","DOI":"10.1126\/science.2188360"},{"key":"S0007114513002171_ref40","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-005-3443-z"},{"key":"S0007114513002171_ref45","first-page":"746","article-title":"The role of lipid peroxidation in endotoxin-induced hepatic damage and the protective effect of antioxidants","volume":"101","author":"Sugino","year":"1987","journal-title":"Surgery"},{"key":"S0007114513002171_ref9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/jn\/134.3.489","article-title":"Glutathione metabolism and its implications for health","volume":"134","author":"Wu","year":"2004","journal-title":"J Nutr"},{"key":"S0007114513002171_ref36","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2000.02207.x"},{"key":"S0007114513002171_ref1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840190525"},{"key":"S0007114513002171_ref31","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1093\/jn\/131.8.2139","article-title":"Inflammatory responses to lipopolysaccharide are suppressed in 40\u00a0% energy-restricted mice","volume":"131","author":"Matsuzaki","year":"2001","journal-title":"J Nutr"},{"key":"S0007114513002171_ref29","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-011-0924-0"},{"key":"S0007114513002171_ref42","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01267.2001"},{"key":"S0007114513002171_ref44","doi-asserted-by":"publisher","DOI":"10.1007\/BF01658282"},{"key":"S0007114513002171_ref27","first-page":"165","article-title":"Decreased sperm quality in mice fed a diet with fish oil","volume":"2","author":"Yi","year":"2011","journal-title":"J Anim Sci Biotechnol"},{"key":"S0007114513002171_ref33","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1007347"},{"key":"S0007114513002171_ref16","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511000249"},{"key":"S0007114513002171_ref5","doi-asserted-by":"publisher","DOI":"10.3109\/08977198908997998"},{"key":"S0007114513002171_ref28","doi-asserted-by":"publisher","DOI":"10.1021\/bi00851a033"},{"key":"S0007114513002171_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2007.02.002"},{"key":"S0007114513002171_ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03571.x"},{"key":"S0007114513002171_ref17","doi-asserted-by":"publisher","DOI":"10.2741\/3783"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114513002171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T23:17:46Z","timestamp":1688339866000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114513002171\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,8]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1,14]]}},"alternative-id":["S0007114513002171"],"URL":"https:\/\/doi.org\/10.1017\/s0007114513002171","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,8]]}}}