{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T21:01:16Z","timestamp":1773435676390,"version":"3.50.1"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2014,1,6]],"date-time":"2014-01-06T00:00:00Z","timestamp":1388966400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2014,4,14]]},"abstract":"<jats:p>Hepcidin, a key regulator of Fe homeostasis, is an ideal drug target for treating patients with Fe disorders such as haemochromatosis, anaemia of chronic inflammation and Fe-deficiency anaemia. However, whether (and how) traditional Chinese black foods (e.g. black soyabeans) target hepcidin and improve Fe-deficiency anaemia remains unclear. Herein, we report that black soyabean seed coat extract (BSSCE) can potently inhibit the<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>expression of hepcidin. In the present study, in cells treated with 200\u00a0\u03bcg\/ml BSSCE, hepcidin expression was found to be reduced to only 6\u00a0% of the control levels (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b701). An AIN-76A diet containing 2\u00a0% BSSCE was fed to 8-week-old male C57BL\/6 mice for 0, 1, 7, 15 or 30\u00a0d; importantly, compared with the day 0 group, the day 7 group exhibited nearly a 50\u00a0% decrease in hepatic hepcidin expression (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b701), a 35\u00a0% decrease in splenic Fe concentrations (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b705) and a 135\u00a0% increase in serum Fe concentrations (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b705). Mechanistically, the effect of BSSCE on hepcidin expression was mediated via a reduction in the phosphorylation levels of mothers against decapentaplegic homolog proteins (Smad)1\/5\/8. Consequently, the mice in the day 30 group exhibited large increases in erythrocyte counts (111\u00a0%<jats:italic>v.<\/jats:italic>day 0,<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b701), Hb concentrations (109\u00a0%,<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b701) and haematocrit values (108\u00a0%,<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b701). In conclusion, these results indicate that black soyabean extract regulates Fe metabolism by inhibiting the expression of hepcidin. This finding can be used to optimise the intervention of patients with hepcidin-related diseases, including Fe-deficiency anaemia.<\/jats:p>","DOI":"10.1017\/s0007114513004005","type":"journal-article","created":{"date-parts":[[2014,1,6]],"date-time":"2014-01-06T11:48:45Z","timestamp":1389008925000},"page":"1181-1189","source":"Crossref","is-referenced-by-count":17,"title":["Black soyabean seed coat extract regulates iron metabolism by inhibiting the expression of hepcidin"],"prefix":"10.1017","volume":"111","author":[{"given":"Mingdao","family":"Mu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aimin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fudi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2014,1,6]]},"reference":[{"key":"S0007114513004005_ref10","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.54"},{"key":"S0007114513004005_ref21","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-160184"},{"key":"S0007114513004005_ref27","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-143354"},{"key":"S0007114513004005_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.06.028"},{"key":"S0007114513004005_ref14","doi-asserted-by":"publisher","DOI":"10.1124\/mol.112.083428"},{"key":"S0007114513004005_ref24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038845"},{"key":"S0007114513004005_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.01.014"},{"key":"S0007114513004005_ref8","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23110"},{"key":"S0007114513004005_ref19","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-06-289082"},{"key":"S0007114513004005_ref15","doi-asserted-by":"publisher","DOI":"10.6090\/jarq.38.213"},{"key":"S0007114513004005_ref1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.69.031905.164337"},{"key":"S0007114513004005_ref17","doi-asserted-by":"publisher","DOI":"10.2174\/157016310791162767"},{"key":"S0007114513004005_ref13","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21675"},{"key":"S0007114513004005_ref30","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-200111000-00008"},{"key":"S0007114513004005_ref28","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2012.16.4.249"},{"key":"S0007114513004005_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/0277-9536(84)90102-3"},{"key":"S0007114513004005_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.10.080"},{"key":"S0007114513004005_ref11","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-345066"},{"key":"S0007114513004005_ref32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151179498"},{"key":"S0007114513004005_ref9","doi-asserted-by":"publisher","DOI":"10.3945\/jn.113.174201"},{"key":"S0007114513004005_ref18","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1182\/blood.V33.3.430.430","article-title":"Food iron absorption: a comparison of vegetable and animal foods","volume":"33","author":"Layrisse","year":"1969","journal-title":"Blood"},{"key":"S0007114513004005_ref23","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1093\/jn\/107.7.1340","article-title":"American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc Committee on Standards for Nutritional Studies","volume":"107","journal-title":"J Nutr"},{"key":"S0007114513004005_ref31","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-02-411470"},{"key":"S0007114513004005_ref7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-08-106195"},{"key":"S0007114513004005_ref25","doi-asserted-by":"publisher","DOI":"10.1038\/ng.335"},{"key":"S0007114513004005_ref3","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-330324"},{"key":"S0007114513004005_ref22","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20121466"},{"key":"S0007114513004005_ref20","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-313064"},{"key":"S0007114513004005_ref5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-258467"},{"key":"S0007114513004005_ref12","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-271791"},{"key":"S0007114513004005_ref2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104742"},{"key":"S0007114513004005_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2004.03.005"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114513004005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T11:49:44Z","timestamp":1597319384000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114513004005\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,6]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,4,14]]}},"alternative-id":["S0007114513004005"],"URL":"https:\/\/doi.org\/10.1017\/s0007114513004005","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,6]]}}}