{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T02:59:34Z","timestamp":1783825174243,"version":"3.55.0"},"reference-count":63,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2014,5,20]],"date-time":"2014-05-20T00:00:00Z","timestamp":1400544000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2014,8,14]]},"abstract":"<jats:p>The effect of high-grain (HG) feeding on caecal bacterial microbiota composition and fermentation and mucosa health is largely unknown. In the present study, ten male goats were randomly assigned to either a group fed a hay diet (0\u00a0% grain;<jats:italic>n<\/jats:italic>5) or a group fed a HG diet (65\u00a0% grain;<jats:italic>n<\/jats:italic>5) to characterise the changes in the composition of the bacterial community and mucosal morphology in the caecum. After 7 weeks of feeding, the HG diet decreased the caecal pH (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7001) and increased (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7001 to<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7004) the caecal digesta concentrations of total volatile fatty acids and lipopolysaccharide (LPS). Pyrosequencing of the<jats:italic>16S<\/jats:italic>ribosomal RNA gene revealed that HG feeding increased (<jats:italic>P<\/jats:italic>=\u00a00\u00b7001 to<jats:italic>P<\/jats:italic>=\u00a00\u00b7009) the abundance of predominant genera<jats:italic>Turicibacter<\/jats:italic>and<jats:italic>Clostridium<\/jats:italic>in the caecal lumen and in the caecal mucosa and decreased (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7001 to<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7009) the proportion of<jats:italic>Bacteroides<\/jats:italic>in the lumen and<jats:italic>Mucispirillum<\/jats:italic>in the mucosa compared with the hay diet. Furthermore, the HG diet-fed goats exhibited intense epithelial damage and up-regulation (<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7001 to<jats:italic>P<\/jats:italic>&lt;\u00a00\u00b7025) of the relative mRNA expression of<jats:italic>IL-1\u03b2<\/jats:italic>,<jats:italic>IL-6<\/jats:italic>,<jats:italic>IL-12<\/jats:italic>and interferon-\u03b3 (<jats:italic>IFN-\u03b3<\/jats:italic>) in the caecal mucosa. The correlation analysis revealed that alterations in caecal pH, LPS concentration and mucosa-associated microbiota abundance during HG feeding might partly contribute to local inflammation. Collectively, these results provide insight into the adaptive response of caecal bacterial populations to HG feeding in goats and reveal that the fermentable substrates that flow into the caecum may cause dramatic alterations in microbial compositions and play a significant role in caecal dysfunction.<\/jats:p>","DOI":"10.1017\/s0007114514000993","type":"journal-article","created":{"date-parts":[[2014,5,20]],"date-time":"2014-05-20T14:29:28Z","timestamp":1400596168000},"page":"416-427","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":109,"title":["High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats"],"prefix":"10.1017","volume":"112","author":[{"given":"Junhua","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyong","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,5,20]]},"reference":[{"key":"S0007114514000993_ref38","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02988-10"},{"key":"S0007114514000993_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm864"},{"key":"S0007114514000993_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2011.12.004"},{"key":"S0007114514000993_ref17","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-38"},{"key":"S0007114514000993_ref29","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.131"},{"key":"S0007114514000993_ref42","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1099\/00207713-52-4-1263","article-title":"Turicibacter sanguinis gen. nov., sp. nov., a novel anaerobic, Gram-positive bacterium","volume":"52","author":"Bosshard","year":"2002","journal-title":"Int J Syst Evol Microbiol"},{"key":"S0007114514000993_ref56","doi-asserted-by":"publisher","DOI":"10.1038\/nri2891"},{"key":"S0007114514000993_ref50","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(05)72807-1"},{"key":"S0007114514000993_ref7","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-4447"},{"key":"S0007114514000993_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/BF00301871"},{"key":"S0007114514000993_ref47","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63472-0"},{"key":"S0007114514000993_ref53","doi-asserted-by":"publisher","DOI":"10.3109\/00365529609094568"},{"key":"S0007114514000993_ref5","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1071\/BI9680177","article-title":"Volatile fatty acids in the caecum of the sheep","volume":"21","author":"Faichney","year":"1968","journal-title":"Aust J Biol Sci"},{"key":"S0007114514000993_ref27","doi-asserted-by":"publisher","DOI":"10.2527\/jas1986.6351634x"},{"key":"S0007114514000993_ref4","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2010-3460"},{"key":"S0007114514000993_ref44","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.2.4.16332"},{"key":"S0007114514000993_ref33","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01102"},{"key":"S0007114514000993_ref24","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(80)83021-9"},{"key":"S0007114514000993_ref60","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1389"},{"key":"S0007114514000993_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2007.04.001"},{"key":"S0007114514000993_ref45","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12092"},{"key":"S0007114514000993_ref10","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0257"},{"key":"S0007114514000993_ref9","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-51-39"},{"key":"S0007114514000993_ref28","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01289-09"},{"key":"S0007114514000993_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2013.08.003"},{"key":"S0007114514000993_ref11","doi-asserted-by":"publisher","DOI":"10.1139\/y06-056"},{"key":"S0007114514000993_ref61","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2013.05.026"},{"key":"S0007114514000993_ref34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504978102"},{"key":"S0007114514000993_ref39","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-012-2188-0"},{"key":"S0007114514000993_ref49","doi-asserted-by":"publisher","DOI":"10.2527\/jas1978.4761329x"},{"key":"S0007114514000993_ref14","first-page":"110","article-title":"Determination of rumen volatile fatty acids by means of gas chromatography","volume":"4","author":"Qin","year":"1982","journal-title":"J Nanjing Agric Coll"},{"key":"S0007114514000993_ref1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00068.2013"},{"key":"S0007114514000993_ref6","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19820038"},{"key":"S0007114514000993_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03864-13"},{"key":"S0007114514000993_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2013.02.005"},{"key":"S0007114514000993_ref30","doi-asserted-by":"crossref","first-page":"1124S","DOI":"10.1093\/ajcn\/73.6.1124S","article-title":"Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium","volume":"73","author":"Lu","year":"2001","journal-title":"Am J Clin Nutr"},{"key":"S0007114514000993_ref13","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-600"},{"key":"S0007114514000993_ref19","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"S0007114514000993_ref57","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.04.022"},{"key":"S0007114514000993_ref2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2012-6187"},{"key":"S0007114514000993_ref20","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8228-8235.2005"},{"key":"S0007114514000993_ref25","doi-asserted-by":"publisher","DOI":"10.2527\/1998.761275x"},{"key":"S0007114514000993_ref48","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.158"},{"key":"S0007114514000993_ref15","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0021-9258(18)51379-X","article-title":"The colorimetric determination of lactic acid in biological material","volume":"138","author":"Barker","year":"1941","journal-title":"J Biol Chem"},{"key":"S0007114514000993_ref43","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00238-13"},{"key":"S0007114514000993_ref46","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.6.2766-2774.2001"},{"key":"S0007114514000993_ref63","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2010-3348"},{"key":"S0007114514000993_ref35","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-013-0192-5"},{"key":"S0007114514000993_ref51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb09016.x"},{"key":"S0007114514000993_ref58","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-172205"},{"key":"S0007114514000993_ref59","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.08.002"},{"key":"S0007114514000993_ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.12.016"},{"key":"S0007114514000993_ref40","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.5"},{"key":"S0007114514000993_ref36","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00366-12"},{"key":"S0007114514000993_ref3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00120.2010"},{"key":"S0007114514000993_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"S0007114514000993_ref55","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21108"},{"key":"S0007114514000993_ref52","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X500643"},{"key":"S0007114514000993_ref23","doi-asserted-by":"publisher","DOI":"10.4236\/abb.2013.410A3002"},{"key":"S0007114514000993_ref41","doi-asserted-by":"publisher","DOI":"10.4141\/A06-065"},{"key":"S0007114514000993_ref54","doi-asserted-by":"crossref","first-page":"E18","DOI":"10.2527\/2005.8313_supplE18x","article-title":"Gastric ulcers in horses","volume":"83","author":"Andrews","year":"2005","journal-title":"J Anim Sci"},{"key":"S0007114514000993_ref26","doi-asserted-by":"publisher","DOI":"10.2527\/1997.753852x"},{"key":"S0007114514000993_ref32","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.112"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114514000993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T23:58:34Z","timestamp":1746230314000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114514000993\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,20]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,8,14]]}},"alternative-id":["S0007114514000993"],"URL":"https:\/\/doi.org\/10.1017\/s0007114514000993","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,20]]},"assertion":[{"value":"Copyright \u00a9 The Authors 2014\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}