{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:58:30Z","timestamp":1789232310937,"version":"build-2803163510"},"reference-count":59,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T00:00:00Z","timestamp":1409875200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2014,10,28]]},"abstract":"<jats:p>The present study investigated the effects of fructo-oligosaccharides (FOS) on the bioavailability of Fe from ferric pyrophosphate (FP), a water-insoluble compound, in Fe-deficient anaemic rats that were subjected to a Hb repletion assay. Male Wistar rats (<jats:italic>n<\/jats:italic>64) were fed adequate or low (8\u00a0mg\/kg) Fe diets for 15\u00a0d followed by 1 or 2 weeks of Fe repletion with diets providing 35\u00a0mg Fe\/kg as ferrous sulphate (FS), FP or FP that was mixed with 7\u00b75\u00a0% FOS in the form of yacon flour or Raftilose P95 (RAF), a purified source of FOS. The effects of FOS were observed within the 1st week of the repletion period. Fe bioavailability was improved by FOS supplementation, as measured by Hb regeneration efficiency and hepatic Fe stores, which were more pronounced in the RAF group. Moreover, RAF supplementation resulted in a higher biological value relative to that of the FP group. FOS supplementation resulted in caecal enlargement, in addition to acidification and Fe species redistribution in the caecal contents relative to the control rats. These effects occurred concomitantly with decreased ferroportin (FPN)-1 expression in the caecal mucosa, which was similar in magnitude to that observed in the FS group. Caecum mucosal morphometry was influenced by FOS supplementation, whereas crypt fission and cell proliferation were highest in the caecum of the RAF group. These results reinforce the effects of FOS as Fe bioavailability enhancers in anaemic rats that are sustained by early changes in their caecal environment (decreased mucosal FPN-1 expression and increased Fe absorbability, crypt fission and cellularity).<\/jats:p>","DOI":"10.1017\/s0007114514002165","type":"journal-article","created":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T14:07:58Z","timestamp":1409926078000},"page":"1286-1295","source":"Crossref","is-referenced-by-count":37,"title":["Fructo-oligosaccharides and iron bioavailability in anaemic rats: the effects on iron species distribution, ferroportin-1 expression, crypt bifurcation and crypt cell proliferation in the caecum"],"prefix":"10.1017","volume":"112","author":[{"given":"Alexandre R.","family":"Lobo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo H. S.","family":"Gaievski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"De Carli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana P.","family":"Alvares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia","family":"Colli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,9,5]]},"reference":[{"key":"S0007114514002165_ref24","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/jaoac\/71.5.1017","article-title":"Determination of insoluble, soluble, and total dietary fiber in foods and food products: interlaboratory study","volume":"71","author":"Prosky","year":"1988","journal-title":"J AOAC Int"},{"key":"S0007114514002165_ref50","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/jn\/127.1.130","article-title":"Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats","volume":"127","author":"Campbell","year":"1997","journal-title":"J Nutr"},{"key":"S0007114514002165_ref38","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114510002497"},{"key":"S0007114514002165_ref37","doi-asserted-by":"crossref","first-page":"2524S","DOI":"10.1093\/jn\/137.11.2524S","article-title":"Young adolescents who respond to an inulin-type fructan substantially increase total absorbed calcium and daily calcium accretion to the skeleton","volume":"137","author":"Abrams","year":"2007","journal-title":"J Nutr"},{"key":"S0007114514002165_ref28","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19920048"},{"key":"S0007114514002165_ref6","doi-asserted-by":"publisher","DOI":"10.1177\/153537020422900903"},{"key":"S0007114514002165_ref4","doi-asserted-by":"publisher","DOI":"10.1136\/gut.28.2.171"},{"key":"S0007114514002165_ref39","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511006301"},{"key":"S0007114514002165_ref33","first-page":"200","volume-title":"O ferro e a sa\u00fade das popula\u00e7\u00f5es","author":"Szarfarc","year":"2006"},{"key":"S0007114514002165_ref45","doi-asserted-by":"crossref","first-page":"3033","DOI":"10.1093\/jn\/136.12.3033","article-title":"Supplemental dietary inulin affects the bioavailability of iron in corn and soybean meal to young pigs","volume":"136","author":"Yasuda","year":"2006","journal-title":"J Nutr"},{"key":"S0007114514002165_ref36","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1093\/ajcn\/69.3.544","article-title":"Oligofructose stimulates calcium absorption in adolescents","volume":"69","author":"Van Der Heuvel","year":"1999","journal-title":"Am J Clin Nutr"},{"key":"S0007114514002165_ref26","doi-asserted-by":"publisher","DOI":"10.1159\/000175540"},{"key":"S0007114514002165_ref10","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1093\/jn\/121.11.1730","article-title":"High propionic acid fermentations and mineral accumulation in the cecum of rats adapted to different levels of inulin","volume":"121","author":"Levrat","year":"1991","journal-title":"J Nutr"},{"key":"S0007114514002165_ref57","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)06882-9"},{"key":"S0007114514002165_ref54","doi-asserted-by":"publisher","DOI":"10.1016\/0015-6264(74)90240-5"},{"key":"S0007114514002165_ref44","first-page":"45","volume-title":"O ferro e a sa\u00fade das popula\u00e7\u00f5es","author":"Colli","year":"2006"},{"key":"S0007114514002165_ref19","first-page":"80","volume-title":"Official Methods of Analysis of Association of Official Analytical Chemists, chapter 45","year":"2006"},{"key":"S0007114514002165_ref11","first-page":"G855","article-title":"Cecal fermentations in rats fed oligosaccharides (inulin) are modulated by dietary calcium level","volume":"264","author":"R\u00e9m\u00e9sy","year":"1993","journal-title":"Am J Physiol"},{"key":"S0007114514002165_ref40","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.110528"},{"key":"S0007114514002165_ref5","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1093\/jn\/135.4.837","article-title":"Dietary fructooligosaccharides increase intestinal permeability in rats","volume":"135","author":"Ten Bruggencate","year":"2005","journal-title":"J Nutr"},{"key":"S0007114514002165_ref51","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19870073"},{"key":"S0007114514002165_ref43","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0773.2002.910301.x"},{"key":"S0007114514002165_ref48","doi-asserted-by":"publisher","DOI":"10.1080\/00365520510011489"},{"key":"S0007114514002165_ref53","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3054.2002.1160403.x"},{"key":"S0007114514002165_ref1","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1093\/jn\/125.6.1401","article-title":"Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics","volume":"125","author":"Gibson","year":"2005","journal-title":"J Nutr"},{"key":"S0007114514002165_ref34","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1093\/ajcn\/32.9.1876","article-title":"Effect of fiber from fruits and vegetables on metabolic responses of human subjects. II. Calcium, magnesium, iron and silicon balances","volume":"32","author":"Kelsay","year":"1979","journal-title":"Am J Clin Nutr"},{"key":"S0007114514002165_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.09.006"},{"key":"S0007114514002165_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-7135(99)00113-9"},{"key":"S0007114514002165_ref35","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1093\/ajcn\/35.1.100","article-title":"Components of fiber bind iron in vitro","volume":"35","author":"Fernandez","year":"1982","journal-title":"Am J Clin Nutr"},{"key":"S0007114514002165_ref20","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1093\/jn\/123.11.1939","article-title":"AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on reformulation of the AIN-76A rodent diet","volume":"123","author":"Reeves","year":"1993","journal-title":"J Nutr"},{"key":"S0007114514002165_ref59","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.023499"},{"key":"S0007114514002165_ref56","doi-asserted-by":"publisher","DOI":"10.1007\/s007950000017"},{"key":"S0007114514002165_ref9","doi-asserted-by":"publisher","DOI":"10.3945\/an.111.000976"},{"key":"S0007114514002165_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2009.05.024"},{"key":"S0007114514002165_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2008.08.002"},{"key":"S0007114514002165_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2006.06.019"},{"key":"S0007114514002165_ref15","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507336805"},{"key":"S0007114514002165_ref55","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.6.799"},{"key":"S0007114514002165_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(96)00105-2"},{"key":"S0007114514002165_ref42","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.035717"},{"key":"S0007114514002165_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2009.10.012"},{"key":"S0007114514002165_ref52","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1093\/jn\/130.11.2718","article-title":"Biomarkers of human colonic cell growth are influenced differently by a history of colonic neoplasia and the consumption of acarbose","volume":"130","author":"Weaver","year":"2000","journal-title":"J Nutr"},{"key":"S0007114514002165_ref23","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1093\/jaoac\/87.5.1200","article-title":"Enzymatic, spectrophotometric determination of glucose, fructose, sucrose, and inulin\/oligofructose in foods","volume":"87","author":"Steegmans","year":"2004","journal-title":"J AOAC Int"},{"key":"S0007114514002165_ref3","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002827"},{"key":"S0007114514002165_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61235-5"},{"key":"S0007114514002165_ref25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0021-9258(18)74965-X","article-title":"Spectrophotometric studies II. Preparations from washed blood cells: nitric oxide hemoglobin and sulphemoglobin","volume":"112","author":"Drabkin","year":"1935","journal-title":"J Biol Chem"},{"key":"S0007114514002165_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.11.067"},{"key":"S0007114514002165_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507825128"},{"key":"S0007114514002165_ref46","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/jn\/128.2.175","article-title":"Increases in the concentrations of available iron in response to dietary iron supplementation are associated with changes in crypt cell proliferation in rat large intestine","volume":"128","author":"Lund","year":"1998","journal-title":"J Nutr"},{"key":"S0007114514002165_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3692-3700.2005"},{"key":"S0007114514002165_ref32","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19970164"},{"key":"S0007114514002165_ref14","doi-asserted-by":"crossref","first-page":"LB97","DOI":"10.1096\/fasebj.20.5.LB97","article-title":"Dietary inulin upregulates the expression of iron and zinc membrane transporters in intestinal enterocytes in the pig","volume":"20","author":"Tako","year":"2006","journal-title":"FASEB J"},{"key":"S0007114514002165_ref58","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114508901276"},{"key":"S0007114514002165_ref29","doi-asserted-by":"publisher","DOI":"10.1021\/ac50043a017"},{"key":"S0007114514002165_ref22","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1093\/jn\/130.8.1984","article-title":"Arabinoxylan fiber from a by-product of wheat flour processing behaves physiologically like a soluble, fermentable fiber in the large bowel of rats","volume":"130","author":"Lu","year":"2000","journal-title":"J Nutr"},{"key":"S0007114514002165_ref47","first-page":"G525","article-title":"Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract","volume":"293","author":"Frazer","year":"2007","journal-title":"Am J Physiol"},{"key":"S0007114514002165_ref41","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.130302"},{"key":"S0007114514002165_ref30","doi-asserted-by":"publisher","DOI":"10.1159\/000144877"},{"key":"S0007114514002165_ref21","first-page":"239","article-title":"Avalia\u00e7\u00e3o por m\u00e9todos in vitro e in vivo da biodisponibilidade de sulfato ferroso microencapsulado","volume":"20","author":"Cocato","year":"2007","journal-title":"Braz J Nutr"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114514002165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T11:17:12Z","timestamp":1650107832000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114514002165\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,5]]},"references-count":59,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,10,28]]}},"alternative-id":["S0007114514002165"],"URL":"https:\/\/doi.org\/10.1017\/s0007114514002165","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,5]]}}}