{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:52:27Z","timestamp":1788519147479,"version":"build-2803163510"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T00:00:00Z","timestamp":1421971200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2015,2,28]]},"abstract":"<jats:p>The aim of the present study was to evaluate the effects of heat stress (HS) and methionine supplementation on the markers of stress and on the gene expression levels of uncoupling proteins (<jats:italic>UCP<\/jats:italic>), betaine\u2013homocysteine methyltransferase (<jats:italic>BHMT<\/jats:italic>), cystathionine \u03b2-synthase (<jats:italic>CBS<\/jats:italic>), glutathione synthetase (<jats:italic>GSS<\/jats:italic>) and glutathione peroxidase 7 (<jats:italic>GPx7<\/jats:italic>). Broilers from 1 to 21\u00a0d and from 22 to 42\u00a0d of age were divided into three treatment groups related to methionine supplementation: without methionine supplementation (MD); recommended level of methionine supplementation (DL1); excess methionine supplementation (DL2). The broilers were either kept at a comfortable thermal temperature or exposed to HS (38\u00b0C for 24\u00a0h). During the starter period, we observed the effects of the interaction between diet and environment on the gene expression levels of <jats:italic>UCP<\/jats:italic>, <jats:italic>BHMT<\/jats:italic> and <jats:italic>GSS<\/jats:italic>. Higher gene expression levels of <jats:italic>UCP<\/jats:italic> and <jats:italic>BHMT<\/jats:italic> were observed in broilers that were maintained at thermal comfort conditions and received the MD diet. HS broilers fed the DL1 and DL2 diets had the highest expression level of <jats:italic>GSS<\/jats:italic>. The expression levels of the <jats:italic>CBS<\/jats:italic> and <jats:italic>GPx7<\/jats:italic> genes were influenced by both the environment and methionine supplementation. During the grower period, the gene expression levels of <jats:italic>BHMT<\/jats:italic>, <jats:italic>CBS<\/jats:italic>, <jats:italic>GSS<\/jats:italic> and <jats:italic>GPx7<\/jats:italic> were affected by the diet\u00a0\u00d7\u00a0environment interaction. A higher expression level of <jats:italic>BHMT<\/jats:italic> was observed in broilers maintained at thermal comfort conditions and on the MD diet. HS induced higher expression levels of <jats:italic>CBS<\/jats:italic>, <jats:italic>GSS<\/jats:italic> and <jats:italic>GPx7<\/jats:italic> in broilers that received the DL1 and DL2 diets. The present results suggest that under HS conditions, methionine supplementation could mitigate the effects of stress, since methionine contributed to the increased expression levels of genes related to antioxidant activity.<\/jats:p>","DOI":"10.1017\/s0007114514003535","type":"journal-article","created":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T03:36:01Z","timestamp":1421984161000},"page":"549-559","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":64,"title":["Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers"],"prefix":"10.1017","volume":"113","author":[{"given":"Ana Paula","family":"Del Vesco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane","family":"Gasparino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane de Oliveira","family":"Grieser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittor","family":"Zancanela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Am\u00e9lia Menck","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adhemar Rodrigues","family":"de Oliveira Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2015,1,23]]},"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114514003535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,21]],"date-time":"2019-04-21T00:00:24Z","timestamp":1555804824000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114514003535\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,23]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,2,28]]}},"alternative-id":["S0007114514003535"],"URL":"https:\/\/doi.org\/10.1017\/s0007114514003535","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,23]]},"assertion":[{"value":"Copyright \u00a9 The Authors 2015\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}