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DHA, a long-chain PUFA, has been shown to be helpful in treating inflammatory bowel disease in experimental models by inhibiting the NF-\u03baB pathway. The present study aimed at investigating the specific effect of DHA on the intestinal barrier function in IL-10-deficient mice. IL-10-deficient mice (IL-10<jats:sup>\u2212\u00a0\/\u00a0\u2212<\/jats:sup>) at 16 weeks of age with established colitis were treated with DHA (i.g. 35\u00b75\u00a0mg\/kg per d) for 2 weeks. The severity of their colitis, levels of pro-inflammatory cytokines, epithelial gene expression, the distributions of TJ proteins (occludin and zona occludens (ZO)-1), and epithelial apoptosis in the proximal colon were measured at the end of the experiment. DHA treatment attenuated the established colitis and was associated with reduced infiltration of inflammatory cells in the colonic mucosa, lower mean histological scores and decreased levels of pro-inflammatory cytokines (IL-17, TNF-\u03b1 and interferon-\u03b3). Moreover, enhanced barrier function was observed in the DHA-treated mice that resulted from attenuated colonic permeability, rescued expression and corrected distributions of occludin and ZO-1. The results of the present study indicate that DHA therapy may ameliorate experimental colitis in IL-10<jats:sup>\u2212\u00a0\/\u00a0\u2212<\/jats:sup> mice by improving the intestinal epithelial barrier function.<\/jats:p>","DOI":"10.1017\/s0007114515001294","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T01:23:03Z","timestamp":1435108983000},"page":"181-188","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":50,"title":["DHA protects against experimental colitis in IL-10-deficient mice associated with the modulation of intestinal epithelial barrier 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