{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:38:18Z","timestamp":1790041098145,"version":"4.0.1"},"reference-count":53,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T00:00:00Z","timestamp":1437436800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Modulating the gut microbiota via dietary interventions is a common strategy to enhance the natural defence mechanisms of the host. Several\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies have highlighted the probiotic potential of\n                    <jats:italic>Bifidobacterium thermophilum<\/jats:italic>\n                    RBL67 (RBL67) selected for its anti-\n                    <jats:italic>Salmonella<\/jats:italic>\n                    effects. The present study aimed to investigate the impact of RBL67 alone and combined with fructo-oligosaccharides (FOS) on the gut microbiota of G\u00f6ttingen minipigs. Minipigs were fed a basal diet supplemented with 8 g\/d probiotic powder (1\u00d710\n                    <jats:sup>9<\/jats:sup>\n                    CFU\/g in skim milk matrix) (probiotic diet (PRO)), 8 g\/d probiotic powder plus 8 g\/d FOS (synbiotic diet (SYN)) or 8 g\/d skim milk powder (control), following a cross-sectional study design. Faecal and caecal microbiota compositions were analysed with pyrosequencing of 16S rRNA genes and quantitative PCR. Metabolic activity in the caecum and colon was measured by HPLC. 16S rRNA gene amplicon sequencing revealed that minipig faeces show close similarity to pig microbiota. During the treatments and at the time of killing of animals, RBL67 was consistently detected in faeces, caecum and colon at numbers of 10\n                    <jats:sup>5<\/jats:sup>\n                    \u201310\n                    <jats:sup>6<\/jats:sup>\n                    16S rRNA copies\/g content after feeding PRO and SYN diets. At the time of killing of animals, significantly higher\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    numbers in the caecum and colon of SYN-fed minipigs were measured compared with PRO. Our data indicate that the G\u00f6ttingen minipig may be a suitable model for gut microbiota research in pigs. Data from this first\n                    <jats:italic>in vivo<\/jats:italic>\n                    study of RBL67 colonisation suggest that the combination with FOS may represent a valuable symbiotic strategy to increase probiotic bacteria levels and survival in gastrointestinal tracts for feed and food applications.\n                  <\/jats:p>","DOI":"10.1017\/s0007114515002263","type":"journal-article","created":{"date-parts":[[2015,7,26]],"date-time":"2015-07-26T23:03:09Z","timestamp":1437951789000},"page":"746-755","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":17,"title":["Effect of\n                    <i>Bifidobacterium thermophilum<\/i>\n                    RBL67 and fructo-oligosaccharides on the gut microbiota in G\u00f6ttingen minipigs"],"prefix":"10.1017","volume":"114","author":[{"given":"Sabine A.","family":"Tanner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Lacroix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Del\u2019Homme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Jans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annina","family":"Zihler Berner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annick","family":"Bernalier-Donadille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Chassard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2015,7,21]]},"reference":[{"key":"S0007114515002263_ref19","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-014-0044-y"},{"key":"S0007114515002263_ref30","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"S0007114515002263_ref33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056612"},{"key":"S0007114515002263_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2009.00645.x"},{"key":"S0007114515002263_ref47","first-page":"107","volume-title":"Swine Nutrition","author":"Grieshop","year":"2000"},{"key":"S0007114515002263_ref12","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00191-13"},{"key":"S0007114515002263_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.05.021"},{"key":"S0007114515002263_ref3","first-page":"171","volume-title":"Bergey\u2019s Manual of Systematic Bacteriology: The Actinobacteria","author":"Biavati","year":"2009"},{"key":"S0007114515002263_ref35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012459"},{"key":"S0007114515002263_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2010.05.009"},{"key":"S0007114515002263_ref15","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12186"},{"key":"S0007114515002263_ref7","unstructured":"von Ah U (2006) Identification of Bifidobacterium thermophilum RBL67 isolated from baby feces and partial purification of its bacteriocin. 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