{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:31:14Z","timestamp":1790645474418,"version":"4.1.0"},"reference-count":55,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T00:00:00Z","timestamp":1511740800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2017,12,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cysteine (Cys), a conditionally indispensable amino acid, is required for the detoxification of paracetamol (acetaminophen,<jats:italic>N<\/jats:italic>-acetyl-<jats:italic>para<\/jats:italic>-aminophenol, 4-hydroxy-acetanilide, APAP), a drug of widespread use in older persons. We recently reported that repeated APAP cures could worsen sarcopenia in old rats, likely to be due to the impairment of Cys\/GSH homoeostasis. The aim of the study was to evaluate whether a dietary Cys supplementation during APAP cures could improve Cys\/GSH homoeostasis and thus preserve skeletal muscle. Male 21\u00b75-month-old Wistar rats received three 2-week-long cures of APAP (1 % of diet) alone or with extra Cys (0\u00b75 % of diet), intercalated with washout periods of 2 weeks (APAP and APAP\u2013Cys groups, respectively). They were compared with untreated control rats (CT group). CT and APAP\u2013Cys groups were pair-fed to the APAP group. Dietary Cys supplementation was efficient to prevent increase in liver mass (<jats:italic>P<\/jats:italic>&lt;0\u00b70001), decrease in liver GSH (<jats:italic>P<\/jats:italic>&lt;0\u00b70001), increase in blood GSH concentration (<jats:italic>P<\/jats:italic>&lt;0\u00b70001), and to some extent, decrease in plasma free Cys concentration (<jats:italic>P<\/jats:italic>&lt;0\u00b705), all induced by repeated APAP cures. The addition of Cys to APAP cures decreased plasma alanine transaminase (<jats:italic>P<\/jats:italic>&lt;0\u00b705), the fractional synthesis rate of liver proteins (<jats:italic>P<\/jats:italic>&lt;0\u00b701), and increased masses of extensor digitorum longus (<jats:italic>P<\/jats:italic>&lt;0\u00b701), and soleus (<jats:italic>P<\/jats:italic>&lt;0\u00b705), compared with the APAP group. Cys supplementation prevented alteration in Cys\/GSH homoeostasis and increased some muscle masses in old rats under repeated cures with a non-toxic dose of APAP.<\/jats:p>","DOI":"10.1017\/s0007114517002847","type":"journal-article","created":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T03:32:06Z","timestamp":1511753526000},"page":"889-896","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Dietary supplementation with cysteine prevents adverse metabolic outcomes of repeated cures with paracetamol in old rats"],"prefix":"10.1017","volume":"118","author":[{"given":"Carole","family":"Mast","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charl\u00e8ne","family":"Pourpe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Voyard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"R\u00e9mond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Mign\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Centeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Dardevet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Savary-Auzeloux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Papet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,11,27]]},"reference":[{"key":"S0007114517002847_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2015.02.009"},{"key":"S0007114517002847_ref37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081495"},{"key":"S0007114517002847_ref54","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/ajcn\/83.2.291","article-title":"Methionine kinetics are altered in the elderly both in the basal state and after vaccination","volume":"83","author":"Mercier","year":"2006","journal-title":"Am J Clin Nutr"},{"key":"S0007114517002847_ref52","first-page":"38","article-title":"Current nutritional recommendations and novel dietary strategies to manage sarcopenia","volume":"2","author":"Calvani","year":"2013","journal-title":"J Frailty Aging"},{"key":"S0007114517002847_ref16","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2009.240"},{"key":"S0007114517002847_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-5614(98)80292-6"},{"key":"S0007114517002847_ref14","doi-asserted-by":"publisher","DOI":"10.1517\/14740338.6.4.341"},{"key":"S0007114517002847_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2012.07.006"},{"key":"S0007114517002847_ref15","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2010.151"},{"key":"S0007114517002847_ref38","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/jn\/128.1.97","article-title":"Metabolism of cysteine is modified during the acute phase of sepsis in rats","volume":"128","author":"Malmezat","year":"1998","journal-title":"J Nutr"},{"key":"S0007114517002847_ref53","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1093\/ajcn\/16.2.229","article-title":"Further observations on the amino acid requirements of older men. 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