{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:57:11Z","timestamp":1790913431386,"version":"4.1.0"},"reference-count":53,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2017,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dietary phospholipid (PL) supplementation has been shown to reduce lipid accumulation in the tissues of farmed fish; however, the mechanisms underlying this effect are largely unknown. Thus, the present study was conducted to evaluate the potential impacts of PL on hepatic lipid metabolism both<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>. For<jats:italic>in vivo<\/jats:italic>study, four experimental diets \u2013 low lipid and low PL diet, as control diet (LL-LP diet, containing 12 % lipid and 1\u00b75 % PL), low-lipid and high-PL diet (containing 12 % lipid and 8 % PL), high-lipid and low-PL diet (HL-LP diet, containing 20 % lipid and 1\u00b75 % PL) and high-lipid and high-PL diet (HL-HP diet, containing 20 % lipid and 8 % PL) \u2013 were randomly allocated to four groups of large yellow croaker (<jats:italic>Larimichthys crocea<\/jats:italic>) (three cages per group) with similar initial body weight (approximately 8 g). For<jats:italic>in vitro<\/jats:italic>study, primary hepatocytes isolated from large yellow croaker were incubated either with graded levels of phosphatidylcholine (PC) (0\u2013250 \u03bc<jats:sc>m<\/jats:sc>) or small interfering RNA (siRNA) for CTP: choline phosphate cytidylyltranferase<jats:italic>\u03b1<\/jats:italic>(<jats:italic>CCT\u03b1<\/jats:italic>) (siRNA-<jats:italic>CCT\u03b1<\/jats:italic>). Results showed that survival was independent of dietary treatments (<jats:italic>P<\/jats:italic>&gt;0\u00b705). Weight gain and feed efficiency in the HL-HP group were significantly higher than in the LL-LP and HL-LP groups (<jats:italic>P<\/jats:italic>&lt;0\u00b705). High level of dietary PL could markedly reduce abnormal hepatic lipid accumulation induced by the HL-LP diet (<jats:italic>P<\/jats:italic>&lt;0\u00b705). Similarly, compared with the corresponding controls, a significant decrease\/increase in lipid content was observed in primary hepatocytes incubated with PC\/siRNA-<jats:italic>CCT\u03b1<\/jats:italic>(<jats:italic>P<\/jats:italic>&lt;0\u00b705). High level of dietary PL reversed the HL-LP diet-induced increased levels of mRNA of fatty acid uptake and lipid synthesis related genes (<jats:italic>P<\/jats:italic>&lt;0\u00b705). In addition, High level of dietary PL markedly down-regulated the transcript levels of fatty acid oxidation-related genes and enhanced the transcript levels of VLDL assembly-related genes regardless of dietary lipid levels (<jats:italic>P<\/jats:italic>&lt;0\u00b705). Compared with corresponding controls, primary hepatocytes treated with PC showed significantly higher mRNA expression of lipid synthesis and VLDL assembly-related genes and lower mRNA expression of fatty acid oxidation-related genes, with hepatocytes treated with siRNA-<jats:italic>CCT\u03b1<\/jats:italic>exhibiting the opposite trend (<jats:italic>P<\/jats:italic>&lt;0\u00b705). In summary, these results demonstrated that high level of dietary PL might reverse the HL-LP diet-induced abnormal lipid accumulation in the liver through inhibiting fatty acid uptake and lipid synthesis, together with promoting the lipid export at the transcriptional level. Lipid export-promoting effect of PC was confirmed by<jats:italic>in vitro<\/jats:italic>studies. The present study showed for the first time that PL or PC could influence various metabolic pathways to regulate hepatic lipid deposition in fish at least at the transcriptional level.<\/jats:p>","DOI":"10.1017\/s000711451700294x","type":"journal-article","created":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T04:15:53Z","timestamp":1512360953000},"page":"999-1009","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":64,"title":["Regulation of hepatic lipid deposition by phospholipid in large yellow 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