{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T23:23:00Z","timestamp":1793834580490,"version":"4.6.1"},"reference-count":43,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T00:00:00Z","timestamp":1527638400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2018,6,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Blueberry, rich in antioxidant and anti-inflammatory phytochemicals, has been demonstrated to lower inflammatory status in adipose induced by high-fat diet (HFD) and obesity. The effect of blueberry on systemic immune functions has not been examined. C57BL\/6 mice were randomised to one of three diets \u2013 low-fat diet (LFD), HFD and HFD plus 4 % (w\/w) blueberry (HFD+B) \u2013 for 8 or 12 weeks.<jats:italic>Ex vivo<\/jats:italic>T-cell mitogens (concanavalin A (Con A); phytohaemagglutinin), T-cell antibody (anti-CD3; anti-CD3\/CD28)-stimulated T-cell proliferation and cytokine production were assessed. After 8 weeks, both HFD groups weighed more (&gt;4 g) than the LFD group; after 12 weeks, HFD+B-fed mice weighed more (&gt;6 g) and had 41 % more adipose tissue than HFD-fed mice (<jats:italic>P<\/jats:italic>&lt;0\u00b705). After 12 weeks, T-cell proliferation was less in both HFD groups, compared with the LFD group. HFD-associated decrements in T-cell proliferation were partially (10\u201350 %) prevented by blueberry supplementation. At 12 weeks, splenocytes from HFD mice, but not from HFD+B mice, produced 51 % less IL-4 (CD3\/CD28) and 57 % less interferon-<jats:italic>\u03b3<\/jats:italic>(Con A) compared with splenocytes from LFD mice (<jats:italic>P<\/jats:italic>&lt;0\u00b705). In response to lipopolysaccharide challenge, splenocytes from both HFD groups produced 24\u201330 % less IL-6 and 27\u201333 % less TNF-<jats:italic>\u03b1<\/jats:italic>compared with splenocytes from LFD mice (<jats:italic>P<\/jats:italic>&lt;0\u00b705), indicating impaired acute innate immune response. By demonstrating deleterious impacts of HFD feeding on T-cell proliferation and splenocyte immune responses, our results provide insights into how HFD\/obesity can disrupt systemic immune function. The protective effects of blueberry suggest that dietary blueberry can buttress T-cell and systemic immune function against HFD-obesity-associated insults.<\/jats:p>","DOI":"10.1017\/s0007114518001034","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T11:38:24Z","timestamp":1527680304000},"page":"1393-1399","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":27,"title":["Dietary supplementation with blueberry partially restores T-cell-mediated function in high-fat-diet-induced obese mice"],"prefix":"10.1017","volume":"119","author":[{"given":"Erin 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