{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:18:37Z","timestamp":1778901517634,"version":"3.51.4"},"reference-count":51,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T00:00:00Z","timestamp":1566518400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2019,11,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t  <jats:p>The present study explored the mechanism of Zn-methionine (Zn-Met) influencing eggshell quality of laying hens and investigated whether the mechanism was related to Ca deposition. Hyline grey layers (<jats:italic>n<\/jats:italic> 384, 38 weeks old) were divided into four groups: 0 mg Zn\/kg, 40, 80 mg Zn\/kg as Zn-Met, and 80 mg Zn\/kg as zinc sulphate (ZnSO<jats:sub>4<\/jats:sub>). Eggshell quality, Zn contents, Zn-containing enzyme activities and expressions of shell matrix proteins in eggshell gland (ESG) were analysed. Zn-Met treatment at 80 mg\/kg increased (<jats:italic>P<\/jats:italic> &lt; 0\u00b705) egg weight and eggshell strength throughout the experiments. The 80 mg\/kg Zn-Met group (<jats:italic>P<\/jats:italic> &lt; 0\u00b705) had decreased mammillary knob width and larger relative atomic weight percentage of Ca, stronger signal intensity of Ca in linear distribution and the Ca was more evenly distributed in the transversal surface of eggshell. Zn contents (<jats:italic>P<\/jats:italic> &lt; 0\u00b7001) in yolk and serum, Ca, albumin (Alb) levels in ESG as well as carbonic anhydrase (CA) activity in serum (<jats:italic>P<\/jats:italic> &lt; 0\u00b705) and mRNA levels of CA and Ca-binding protein-d<jats:sub>28k<\/jats:sub> (CaBP-D<jats:sub>28k<\/jats:sub>) (<jats:italic>P<\/jats:italic> &lt; 0\u00b7001) in the 80 mg\/kg Zn-Met group were the highest among all treatments. In conclusion, shell strength as one of eggshell qualities was mostly related to mammillary cone width in ultrastructure caused by the pattern of Ca deposition in eggshell formation. Also, the increase in Zn-Met-induced Ca deposition may be due to the increased Zn contents in serum and tissues, which were attributable to the increased CA concentrations in serum, Ca, Alb levels and up-regulated CA and CaBP-D<jats:sub>28k<\/jats:sub> mRNA levels in ESG.<\/jats:p>","DOI":"10.1017\/s000711451900206x","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T01:34:18Z","timestamp":1566524058000},"page":"961-973","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":23,"title":["Dietary addition of zinc-methionine influenced eggshell quality by affecting calcium deposition in eggshell formation of laying hens"],"prefix":"10.1017","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0728-2333","authenticated-orcid":false,"given":"Lanlan","family":"Li","sequence":"first","affiliation":[{"name":"Zhejiang University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Miao","sequence":"additional","affiliation":[{"name":"Zhejiang University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingkun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Zhejiang University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liansong","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Weifeng Biological Technology Co., Ltd"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoting","family":"Zou","sequence":"additional","affiliation":[{"name":"Zhejiang University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2019,8,23]]},"reference":[{"key":"S000711451900206X_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2007.10.003"},{"key":"S000711451900206X_ref40","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.009122"},{"key":"S000711451900206X_ref33","volume-title":"Egg and Eggshell Quality","author":"Solomon","year":"1991"},{"key":"S000711451900206X_ref38","first-page":"59","article-title":"Effect of manganese and zinc chelates on the quality of eggs","volume":"50","author":"Klecker","year":"2002","journal-title":"Acta Univ Agric Silvic Mendel Brun"},{"key":"S000711451900206X_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-8401(02)00051-2"},{"key":"S000711451900206X_ref29","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114517002446"},{"key":"S000711451900206X_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpv.2004.08.002"},{"key":"S000711451900206X_ref4","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pew490"},{"key":"S000711451900206X_ref44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406033200"},{"key":"S000711451900206X_ref42","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03391"},{"key":"S000711451900206X_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(00)00095-0"},{"key":"S000711451900206X_ref41","doi-asserted-by":"publisher","DOI":"10.1159\/000151374"},{"key":"S000711451900206X_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.4.650"},{"key":"S000711451900206X_ref32","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0750656"},{"key":"S000711451900206X_ref30","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"S000711451900206X_ref13","unstructured":"13. Martin, KM (2016) The effects of zinc supplementation from two sources on egg quality and bone health in laying hens. Theses and dissertations in Animal Science, University of Nebraska-Lincoln, Animal Science Department."},{"key":"S000711451900206X_ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2011.02280.x"},{"key":"S000711451900206X_ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-015-0252-4"},{"key":"S000711451900206X_ref5","doi-asserted-by":"publisher","DOI":"10.3382\/japr\/pfv029"},{"key":"S000711451900206X_ref20","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03190"},{"key":"S000711451900206X_ref23","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03354"},{"key":"S000711451900206X_ref37","doi-asserted-by":"publisher","DOI":"10.18174\/194063"},{"key":"S000711451900206X_ref31","doi-asserted-by":"publisher","DOI":"10.17221\/1966-VETMED"},{"key":"S000711451900206X_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(01)00812-7"},{"key":"S000711451900206X_ref28","unstructured":"28. Rubio Zapata, NK (2016) Effect of increasing levels of dietary zinc (Zn), manganese (Mn), and copper (Cu) from organic and inorganic sources on egg quality and egg Zn, Mn, and Cu content in laying hens. Doctoral dissertation, Louisiana State University."},{"key":"S000711451900206X_ref21","volume-title":"Nutrient Requirements of Poultry","year":"1994"},{"key":"S000711451900206X_ref48","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0046-y"},{"key":"S000711451900206X_ref24","first-page":"155","article-title":"Effects of supplementary mineral amino acid chelate (Zn AA-Mn AA) on the laying performance, egg quality and some blood parameters of late laying period layer hens","volume":"21","author":"Yilmaz Dikmen","year":"2015","journal-title":"Kafkas Univ Vet Fakult Dergisi"},{"key":"S000711451900206X_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01794.x"},{"key":"S000711451900206X_ref7","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex042"},{"key":"S000711451900206X_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-010-8779-x"},{"key":"S000711451900206X_ref35","doi-asserted-by":"publisher","DOI":"10.1080\/00071669208417469"},{"key":"S000711451900206X_ref51","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex187"},{"key":"S000711451900206X_ref47","doi-asserted-by":"publisher","DOI":"10.2741\/3985"},{"key":"S000711451900206X_ref8","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pew495"},{"key":"S000711451900206X_ref34","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-635X2005000200001"},{"key":"S000711451900206X_ref39","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.46.267"},{"key":"S000711451900206X_ref11","doi-asserted-by":"publisher","DOI":"10.1385\/BTER:101:2:147"},{"key":"S000711451900206X_ref19","first-page":"630","article-title":"Effects of organic chelates of zinc, manganese and copper in comparison to their inorganic sources on performance of broiler chickens","volume":"6","author":"Gheisari","year":"2010","journal-title":"J Anim Plant Sci"},{"key":"S000711451900206X_ref46","doi-asserted-by":"publisher","DOI":"10.1533\/9780857093912.2.151"},{"key":"S000711451900206X_ref22","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114518001368"},{"key":"S000711451900206X_ref25","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex301"},{"key":"S000711451900206X_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2008.04.008"},{"key":"S000711451900206X_ref12","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey203"},{"key":"S000711451900206X_ref17","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey609"},{"key":"S000711451900206X_ref36","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2007.236.241"},{"key":"S000711451900206X_ref50","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(05)80019-8"},{"key":"S000711451900206X_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-7037(02)00971-7"},{"key":"S000711451900206X_ref2","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey440"},{"key":"S000711451900206X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2016.01.009"},{"key":"S000711451900206X_ref1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex456"}],"updated-by":[{"DOI":"10.1017\/s0007114519003209","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T00:00:00Z","timestamp":1566518400000}}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S000711451900206X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T12:59:26Z","timestamp":1759841966000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S000711451900206X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,23]]},"references-count":51,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["S000711451900206X"],"URL":"https:\/\/doi.org\/10.1017\/s000711451900206x","relation":{"correction":[{"id-type":"doi","id":"10.1017\/S0007114519003209","asserted-by":"object"}]},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,23]]},"assertion":[{"value":"\u00a9 The Authors 2019\u00a0","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}