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There is limited but suggestive evidence that myo-inositol (MI) and probiotics (PB) could improve glucose tolerance during pregnancy. The present study tested the hypothesis that MI and\/or PB supplementation would reduce the risk of glucose intolerance during pregnancy. Female C57BL\/6 mice were randomised to receive either no treatment, MI, PB (<jats:italic>Lactobacillus rhamnosus<\/jats:italic> and <jats:italic>Bifidobacterium lactis<\/jats:italic>) or both (MIPB) for 5 weeks. They were then provided with a high-fat diet for 1 week before mating commenced and throughout mating\/gestation, while remaining on their respective treatments. An oral glucose tolerance test occurred at gestational day (GD) 16\u00b75 and tissue collection at GD 18\u00b75. Neither MI nor PB, separately or combined, improved glucose tolerance. However, MI and PB both independently increased adipose tissue expression of <jats:italic>Ir<\/jats:italic>, <jats:italic>Irs1<\/jats:italic>, <jats:italic>Akt2<\/jats:italic> and <jats:italic>Pck1<\/jats:italic>, and PB also increased <jats:italic>Ppar\u03b3<\/jats:italic>. MI was associated with reduced gestational weight gain, whilst PB was associated with increased maternal fasting glucose, total cholesterol and pancreas weight. These results suggest that MI and PB may improve insulin intracellular signalling in adipose tissue but this did not translate to meaningful differences in glucose tolerance. The absence of fasting hyperglycaemia or insulin resistance suggests this is a very mild model of GDM, which may have affected our ability to assess the impact of these nutrients.<\/jats:p>","DOI":"10.1017\/s0007114519003039","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T03:26:40Z","timestamp":1574825200000},"page":"516-528","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":14,"title":["The effects of myo-inositol and probiotic supplementation in a high-fat-fed preclinical model of glucose intolerance in pregnancy"],"prefix":"10.1017","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1421-3387","authenticated-orcid":false,"given":"J. F.","family":"Plows","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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