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Six diets (iso-nitrogenous) differing in Met concentrations (2\u00b754, 4\u00b785, 7\u00b743, 10\u00b712, 12\u00b740 and 15\u00b711 g\/kg diets) were fed to 540 grass carp (178\u00b747 (<jats:sc>SD<\/jats:sc>0\u00b736) g). Results showed (<jats:italic>P &lt;<\/jats:italic>0\u00b705) that compared with Met deficiency, optimal level of dietary Met (1) increased feed intake, feed efficiency, specific growth rate and percentage weight gain (PWG); (2) increased fish muscle protein, lipid and free amino acid contents and improved fish muscle fatty acid profile as well as increased protein content in part associated with the target of rapamycin complex 1 (TORC1)\/S6K1 signalling pathway; (3) increased the frequency distribution of muscle fibre with &gt;50 \u00b5m of diameter; (4) increased type I collagen synthesis partly related to the transforming growth factor-\u03b21\/Smads and CK2\/TORC1 signalling pathways. In conclusion, dietary Met improved muscle growth, which might be due to the regulation of muscle nutritive deposition, muscle fibre growth and type I collagen synthesis-related signal molecules. Finally, according to PWG and muscle collagen content, the Met requirements for on-growing grass carp (178\u2013626 g) were estimated to be 9\u00b756 g\/kg diet (33\u00b726 g\/kg protein of diet) and 9\u00b728 g\/kg diet (32\u00b729 g\/kg of dietary protein), respectively.<\/jats:p>","DOI":"10.1017\/s0007114520002998","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T03:48:54Z","timestamp":1595908134000},"page":"321-336","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":58,"title":["Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (<i>Ctenopharyngodon idella<\/i>)"],"prefix":"10.1017","volume":"126","author":[{"given":"Chuang-Chuang","family":"Fang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Dan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Yao","family":"Kuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-An","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Qiu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"S0007114520002998_ref58","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513002419"},{"key":"S0007114520002998_ref18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401238200"},{"key":"S0007114520002998_ref19","doi-asserted-by":"publisher","DOI":"10.1111\/are.13230"},{"key":"S0007114520002998_ref27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2095.2008.00613.x"},{"key":"S0007114520002998_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(05)80072-2"},{"key":"S0007114520002998_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.12.003"},{"key":"S0007114520002998_ref88","doi-asserted-by":"publisher","DOI":"10.1360\/N052018-00132"},{"key":"S0007114520002998_ref62","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/864757"},{"key":"S0007114520002998_ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115129"},{"key":"S0007114520002998_ref17","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114514001226"},{"key":"S0007114520002998_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.10.074"},{"key":"S0007114520002998_ref20","first-page":"2263","article-title":"Methionine requirement of grass carp (Ctenopharyngodon idell) during middle growth stage","volume":"24","author":"Tang","year":"2012","journal-title":"Chin J Anim Nutr"},{"key":"S0007114520002998_ref6","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.1837"},{"key":"S0007114520002998_ref90","unstructured":"90. 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