{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:45:21Z","timestamp":1793745921219,"version":"4.6.1"},"reference-count":53,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T00:00:00Z","timestamp":1636675200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2022,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diarrhoea caused by pathogens such as enterotoxigenic <jats:italic>E. coli<\/jats:italic> (ETEC) is a serious threat to the health of young animals and human infants. Here, we investigated the protective effect of fructo-oligosaccharides (FOS) on the intestinal epithelium with ETEC challenge in a weaned piglet model. Twenty-four weaned piglets were randomly divided into three groups: (1) non-ETEC-challenged control (CON); (2) ETEC-challenged control (ECON); and (3) ETEC challenge + 2\u00b75 g\/kg FOS (EFOS). On day 19, the CON pigs were orally infused with sterile culture, while the ECON and EFOS pigs were orally infused with active ETEC (2\u00b75 \u00d7 109 colony-forming units). On day 21, pigs were slaughtered to collect venous blood and small intestine. Result showed that the pre-treatment of FOS improved the antioxidant capacity and the integrity of intestinal barrier in the ETEC-challenged pigs without affecting their growth performance. Specifically, compared with ECON pigs, the level of GSH peroxidase and catalase in the plasma and intestinal mucosa of EFOS pigs was increased (<jats:italic>P<\/jats:italic> &lt; 0\u00b705), and the intestinal barrier marked by zonula occluden-1 and plasmatic diamine oxidase was also improved in EFOS pigs. A lower level (<jats:italic>P<\/jats:italic> &lt; 0\u00b705) of inflammatory cytokines in the intestinal mucosa of EFOS pigs might be involved in the inhibition of TLR4\/MYD88\/NF-<jats:italic>\u03ba<\/jats:italic>B pathway. The apoptosis of jejunal cells in EFOS pigs was also lower than that in ECON pigs (<jats:italic>P<\/jats:italic> &lt; 0\u00b705). Our findings provide convincing evidence of possible prebiotic and protective effect of FOS on the maintenance of intestinal epithelial function under the attack of pathogens.<\/jats:p>","DOI":"10.1017\/s0007114521004451","type":"journal-article","created":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T06:53:00Z","timestamp":1636699980000},"page":"1526-1534","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":13,"title":["Dietary supplementation of fructo-oligosaccharides alleviates enterotoxigenic <i>E. coli<\/i>-induced disruption of intestinal epithelium in a weaned piglet 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