{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T07:54:13Z","timestamp":1784447653434,"version":"3.55.0"},"reference-count":69,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T00:00:00Z","timestamp":1643932800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2022,12,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to document the method standardisation and assessment of micronutrient and inflammatory markers in women from indigenous tribal communities of Jharkhand using a low-volume, high-throughput assay. This cross-sectional study was done among women of the reproductive age group from Sauria Paharia and Santhal tribal households (HH) in selected villages. Capillary blood samples were collected from the women during a HH survey to estimate ferritin, soluble transferrin receptor, retinol binding protein 4 and inflammatory biomarkers, C-reactive protein (CRP) and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -1-acid glycoprotein (AGP) using a multiplex assay. Vitamin D and Hb were estimated using an LC-MS technique and cyanmethaemoglobin method, respectively. A multiplex Luminex-based method was developed and standardised. The assay was used to estimate biomarkers in samples from 413 women (178 and 235 from Sauria Paharia and Santhal tribes, respectively). Over 51 % of women had raised CRP or AGP levels. Fe status was significantly better in Sauria Paharia compared with the Santhal women. Anaemia prevalence was 72 % among Santhal women. The proportion of women with Fe deficiency increased after adjusting for inflammation. The overall prevalence of vitamin A deficiency and insufficiency was 25 and 34 %, respectively, with similar prevalence in both tribes. All Santhal women had sufficient vitamin D levels, while 25 and 20 % of Sauria Paharia women had insufficient and deficient vitamin D levels, respectively. Our low-volume, high-throughput multiplex assays may provide a feasible approach for assessing nutritional biomarkers in nutritionally vulnerable hard-to-reach communities.\n                  <\/jats:p>","DOI":"10.1017\/s0007114522000320","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T04:58:40Z","timestamp":1643950720000},"page":"2464-2479","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Standardisation and application of a novel multiplex assay for estimating micronutrient status and inflammatory markers in women of Sauria Paharia and Santhal tribes of Jharkhand"],"prefix":"10.1017","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6487-6632","authenticated-orcid":false,"given":"Archna","family":"Singh","sequence":"first","affiliation":[{"name":"All India Institute of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivasankar","family":"Baalasubramanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mani","family":"Kalaivani","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8627-4106","authenticated-orcid":false,"given":"Ridhima","family":"Kapoor","sequence":"additional","affiliation":[{"name":"Public Health Foundation of India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketaki","family":"Bhagwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2229-4455","authenticated-orcid":false,"given":"Suparna","family":"Ghosh-Jerath","sequence":"additional","affiliation":[{"name":"Public Health Foundation of India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,2,4]]},"reference":[{"key":"S0007114522000320_ref37","volume-title":"IBM SPSS Statistics for Windows, Version 24.0","year":"2016"},{"key":"S0007114522000320_ref6","volume-title":"United Nations Declaration on the Rights of Indigenous Peoples United Nations for Indigenous Peoples","year":"2007"},{"key":"S0007114522000320_ref10","doi-asserted-by":"crossref","first-page":"345","DOI":"10.4103\/2249-4863.123881","article-title":"Prevalence of anemia among tribal women of reproductive age in Udupi Taluk, Karnataka","volume":"2","author":"Kamath","year":"2013","journal-title":"J Family Med Prim Care"},{"key":"S0007114522000320_ref2","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-015-2058-x"},{"key":"S0007114522000320_ref22","unstructured":"22. World Health Organisation (2011) Serum Ferritin Concentrations for the Assessment of Iron Status and Iron Deficiency in Populations. Vitamin and Mineral Nutrition Information System. http:\/\/wwwwhoint\/vmnis\/indicators\/serum_ferritinw.pdf (accessed January 2021)."},{"key":"S0007114522000320_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/mcn.12558"},{"key":"S0007114522000320_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2010.02.012"},{"key":"S0007114522000320_ref31","unstructured":"31. World Health Organisation (2011) Haemoglobin Concentrations for the Diagnosis of Anaemia and assessment of severity. Vitamin and Mineral Nutrition Information System. https:\/\/www.who.int\/vmnis\/indicators\/haemoglobin.pdf (accessed January 2021)."},{"key":"S0007114522000320_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.worlddev.2016.08.011"},{"key":"S0007114522000320_ref16","doi-asserted-by":"publisher","DOI":"10.1080\/03670244.2015.1017758"},{"key":"S0007114522000320_ref63","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-019-6963-2"},{"key":"S0007114522000320_ref55","first-page":"52","article-title":"Development of Non-alcoholic Fatty Liver Disease (NAFLD) in young obese tribal subjects of Tripura: link between low 25 (OH) Vitamin-D levels and immune modulators","volume":"67","author":"Chakraborty","year":"2019","journal-title":"J Assoc Phys India"},{"key":"S0007114522000320_ref19","first-page":"1","article-title":"Agroforestry diversity, indigenous food consumption and nutritional outcomes in Sauria Paharia tribal women of Jharkhand, India","volume":"17","author":"Ghosh-Jerath","year":"2020","journal-title":"Matern Child Nutr"},{"key":"S0007114522000320_ref26","unstructured":"26. World Health Organisation (2002) World Health Survey 2002. https:\/\/www.who.int\/healthinfo\/survey\/whsshortindividuala.pdf?ua=1 (accessed January 2021)."},{"key":"S0007114522000320_ref67","unstructured":"67. MOHFW (2021) Malaria: National Vector Borne Disease Control Programme (NVBDCP). https:\/\/nvbdcp.gov.in\/index1.php?lang=1&level=1&sublinkid=5784&lid=3689 (accessed October 2021)."},{"key":"S0007114522000320_ref4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.16.070196.002221"},{"key":"S0007114522000320_ref54","first-page":"211","article-title":"Vitamin D status in Andhra Pradesh : a population based study","volume":"127","author":"Harinarayan","year":"2008","journal-title":"Indian J Med Res"},{"key":"S0007114522000320_ref46","volume-title":"District Level Household Survey on Reproductive and Child Health, India 2002\u20132004","year":"2006"},{"key":"S0007114522000320_ref41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185868"},{"key":"S0007114522000320_ref50","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.117.155960"},{"key":"S0007114522000320_ref61","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0278"},{"key":"S0007114522000320_ref49","doi-asserted-by":"publisher","DOI":"10.3945\/jn.112.167775"},{"key":"S0007114522000320_ref17","doi-asserted-by":"publisher","DOI":"10.1017\/S1368980016000318"},{"key":"S0007114522000320_ref42","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0215782"},{"key":"S0007114522000320_ref9","volume-title":"Jharkhand, Data Highlights: The Scheduled Tribes","year":"2001"},{"key":"S0007114522000320_ref29","doi-asserted-by":"publisher","DOI":"10.1080\/00365510500206542"},{"key":"S0007114522000320_ref65","volume-title":"Assessing the Iron Status of Populations: Including Literature Reviews","year":"2007"},{"key":"S0007114522000320_ref21","unstructured":"21. Directorate General of Health Services, Ministry of Health and Family Welfare (2021) Government of India National Vector Borne Disease Control Programme Annual Report 2014\u201315. https:\/\/nvbdcp.gov.in\/Doc\/Annual-report-NVBDCP-2014\u201315.pdf (accessed January 2021)."},{"key":"S0007114522000320_ref60","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.143073"},{"key":"S0007114522000320_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00345-7"},{"key":"S0007114522000320_ref34","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.145805"},{"key":"S0007114522000320_ref53","doi-asserted-by":"crossref","first-page":"729","DOI":"10.3390\/nu6020729","article-title":"Vitamin D deficiency in India: prevalence, causalities and interventions","volume":"6","author":"Gupta","year":"2014","journal-title":"Nutrients"},{"key":"S0007114522000320_ref33","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.142166"},{"key":"S0007114522000320_ref15","volume-title":"National Family Health Survey (NFHS-4), 2015\u20132016: India","year":"2017"},{"key":"S0007114522000320_ref59","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-020-1502-7"},{"key":"S0007114522000320_ref56","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1833-9"},{"key":"S0007114522000320_ref51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1993.tb03225.x"},{"key":"S0007114522000320_ref45","volume-title":"National Family Health Survey (NFHS-4), 2015\u20132016, State Fact Sheet Jharkhand","year":"2017"},{"key":"S0007114522000320_ref7","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2020-002442"},{"key":"S0007114522000320_ref39","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.11.3127"},{"key":"S0007114522000320_ref69","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.142372"},{"key":"S0007114522000320_ref66","unstructured":"66. MOHFW Anemia Mukt Bharat (2021) A Programme by Ministry of Health and UNICEF. Anemia Mukt Bharat Dashboard. https:\/\/anemiamuktbharat.info\/ (accessed October 2021)."},{"key":"S0007114522000320_ref3","doi-asserted-by":"publisher","DOI":"10.3402\/ijch.v70i5.17863"},{"key":"S0007114522000320_ref35","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.141762"},{"key":"S0007114522000320_ref57","first-page":"135","article-title":"Situation analysis of malaria in Godda district of Jharkhand during malaria epidemic","volume":"43","author":"Singh","year":"2011","journal-title":"J Commun Dis"},{"key":"S0007114522000320_ref32","unstructured":"32. WHO, UNICEF & UNU (2001) Iron Deficiency Anaemia. Assessment, Prevention, and Control. A Guide for Programme Managers. https:\/\/www.ihs.org.in\/SHG\/WHO-Anemia%20detection%20guidelines.pdf (accessed January 2021)."},{"key":"S0007114522000320_ref43","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_249_16"},{"key":"S0007114522000320_ref62","doi-asserted-by":"publisher","DOI":"10.4103\/0019-557X.123268"},{"key":"S0007114522000320_ref20","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2020.00061"},{"key":"S0007114522000320_ref14","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_2079_18"},{"key":"S0007114522000320_ref36","volume-title":"Stata Statistical Software: Release 15","year":"2017"},{"key":"S0007114522000320_ref44","doi-asserted-by":"publisher","DOI":"10.4103\/ijcm.IJCM_356_19"},{"key":"S0007114522000320_ref24","unstructured":"24. Census (2011) Census of India Website: Office of the Registrar General & Census Commissioner, India. http:\/\/censusindia.gov.in\/ (accessed September 2019)."},{"key":"S0007114522000320_ref48","first-page":"58","article-title":"A comparative study on prevalence of anaemia in women by cyanmethaemoglobin and HemoCue methods","volume":"27","author":"Mohanram","year":"2002","journal-title":"Indian J Community Med"},{"key":"S0007114522000320_ref25","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-019-6963-2"},{"key":"S0007114522000320_ref1","unstructured":"1. International Labour Organisation (2016) Who are the Indigenous and Tribal Peoples? http:\/\/www.ilo.org\/global\/topics\/indigenous-tribal\/WCMS_503321\/lang--en\/index.htm (accessed January 2021)."},{"key":"S0007114522000320_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/mcn.12557"},{"key":"S0007114522000320_ref23","doi-asserted-by":"publisher","DOI":"10.3945\/jn.114.194571"},{"key":"S0007114522000320_ref30","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.142273"},{"key":"S0007114522000320_ref5","doi-asserted-by":"publisher","DOI":"10.1017\/S1368980014002961"},{"key":"S0007114522000320_ref47","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-88425-y"},{"key":"S0007114522000320_ref40","doi-asserted-by":"publisher","DOI":"10.1017\/S136898001300222X"},{"key":"S0007114522000320_ref52","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13004"},{"key":"S0007114522000320_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2016.11.003"},{"key":"S0007114522000320_ref18","doi-asserted-by":"publisher","DOI":"10.1177\/0379572118805652"},{"key":"S0007114522000320_ref58","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19970192"},{"key":"S0007114522000320_ref64","first-page":"1","article-title":"Poor and state entitlements: a case study of selected tribes of Jharkhand","volume":"5","author":"Gupta","year":"2013","journal-title":"Jharkhand J Soc Dev"},{"key":"S0007114522000320_ref68","unstructured":"68. MOHFW (2021) National Vitamin A Prophylaxis Program. National Health Portal of India. https:\/\/www.nhp.gov.in\/national-vitamin-a-prophylaxis-program_pg (accessed October 2021)."},{"key":"S0007114522000320_ref38","doi-asserted-by":"publisher","DOI":"10.3945\/an.115.010215"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114522000320","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T09:17:56Z","timestamp":1783070276000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114522000320\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,4]]},"references-count":69,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12,28]]}},"alternative-id":["S0007114522000320"],"URL":"https:\/\/doi.org\/10.1017\/s0007114522000320","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,4]]},"assertion":[{"value":"\u00a9 The Author(s), 2022. Published by Cambridge University Press on behalf of The Nutrition Society","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}