{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T22:36:19Z","timestamp":1787610979967,"version":"build-2736575974"},"reference-count":62,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2023,2,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adipocyte expansion through adipogenesis can offset the adverse metabolic effects of obesity.<jats:italic>Nigella sativa<\/jats:italic>(NS) (black seed) oil is shown to have therapeutic features in the management of obesity. NS oil might have beneficial changes in obese populations through mediating serum levels of adipogenesis-related parameters and relative transcriptional gene\u2013diet interactions (nutrigenomics), though no previous studies assessed this mechanism in overweight\/obese participants. This study assessed the effects of NS oil supplements on blood concentration and mRNA expression levels of TNF-<jats:italic>\u03b1<\/jats:italic>, PPAR-<jats:italic>\u03b3<\/jats:italic>and serum adiponectin and expression of<jats:italic>AdipoR1<\/jats:italic>, as major adipogenesis and obesity-related parameters, in overweight\/obese women using a cross-over design. Eligible women were randomised to receive either NS oil supplements (2000 mg\/d) or placebo. Two periods of interventions (8 weeks in each) were cross-changed by a 4-week washout period. An individualised diet plan without calorie deficits was given to participants to match their energy\/macronutrient intakes. The Pkcross procedure and intention-to-treat analysis were performed using Stata.<jats:italic>Cohen\u2019s d(d)<\/jats:italic>was estimated to measure the magnitude of the effects. Forty-six participants were included. NS oil capsules reduced transcription levels ((<jats:italic>d<\/jats:italic>= \u20132\u00b731),<jats:italic>P<\/jats:italic>&lt; 0\u00b7001) and blood concentrations of TNF-<jats:italic>\u03b1<\/jats:italic>((<jats:italic>d<\/jats:italic>= \u20130\u00b729),<jats:italic>P<\/jats:italic>&lt; 0\u00b7001). AdipoR1 expression (<jats:italic>d<\/jats:italic>= 2\u00b724,<jats:italic>P<\/jats:italic>&lt; 0\u00b7001) and serum adiponectin (<jats:italic>d<\/jats:italic>= 0\u00b788,<jats:italic>P<\/jats:italic>&lt; 0\u00b7001) showed a significant augmentation with a medium-high effect size, as did gene expression (<jats:italic>d<\/jats:italic>= 0\u00b769,<jats:italic>P<\/jats:italic>&lt; 0\u00b7001) and serum levels of PPAR-<jats:italic>\u03b3<\/jats:italic>(<jats:italic>d<\/jats:italic>= 0\u00b797,<jats:italic>P<\/jats:italic>&lt; 0\u00b7001). There was a moderate but significant decrease in body weight (<jats:italic>d<\/jats:italic>= 0\u00b76,<jats:italic>P<\/jats:italic>&lt; 0\u00b7001). The present beneficial findings would provide strong information for future nutrigenomics\/clinical trial studies assessing the role of NS in the management of obesity and other comorbidities.<\/jats:p>","DOI":"10.1017\/s0007114522001428","type":"journal-article","created":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T04:47:15Z","timestamp":1652244435000},"page":"627-636","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Effects of<i>Nigella sativa<\/i>supplementation on blood concentration and mRNA expression of TNF-<i>\u03b1<\/i>, PPAR-<i>\u03b3<\/i>and adiponectin, as major adipogenesis-related markers, in obese and overweight women: a crossover, randomised-controlled trial"],"prefix":"10.1017","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5476-2973","authenticated-orcid":false,"given":"Elham","family":"Razmpoosh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Safi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azadeh","family":"Nadjarzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Salehi-Abargouei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahta","family":"Mazaheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2391-4924","authenticated-orcid":false,"given":"Parvin","family":"Mirmiran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Meyre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"S0007114522001428_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2020.102653"},{"key":"S0007114522001428_ref14","doi-asserted-by":"publisher","DOI":"10.1186\/s13063-019-3568-0"},{"key":"S0007114522001428_ref59","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104367"},{"key":"S0007114522001428_ref2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0093-z"},{"key":"S0007114522001428_ref27","unstructured":"27. 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