{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:14:58Z","timestamp":1791166498303,"version":"4.1.0"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2008,2,18]],"date-time":"2008-02-18T00:00:00Z","timestamp":1203292800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bull. Entomol. Res."],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper describes the development of rapid immunodiagnostic tests for the detection of storage mite infestations in cereals and cereal products. The study's first phase (proof of concept) involved the production of a species-specific enzyme-linked immunoassay (ELISA) for the flour mite, <jats:italic>Acarus siro<\/jats:italic> (L.), a major pest of stored commodities. The specificity of this new assay was assessed against key stored product contaminants (13 species of mites of which three were predatory, five species of insects and five species of fungi) in the presence and absence of grain. The assay was species-specific (no cross-reactivity to other storage contaminants) and was unaffected by the presence of cereal antigens in the extract. In the study's second phase, species- and genera-specific ELISAs were developed for a range of key storage mite pests: the cosmopolitan food mite (<jats:italic>Lepidoglyphus destructor<\/jats:italic>), the grocers' itch mite (<jats:italic>Glycyphagus domesticus<\/jats:italic>), the grainstack mite (<jats:italic>Tyrophagus longior<\/jats:italic>), mites of the <jats:italic>Tyrophagus<\/jats:italic> and <jats:italic>Glycyphagus<\/jats:italic> generas, and all storage mites. All tests were demonstrably specific to target species or genera, with no cross-reactions observed to other storage pest contaminants or cereals. The final, validation phase, involved a comparative assessment of the species-specific <jats:italic>A. siro<\/jats:italic> and the genus-specific <jats:italic>Tyrophagus<\/jats:italic> ELISAs with the flotation technique using laboratory and field samples. Both ELISAs were quantitative (0\u201330 mites per 10 g wheat) and produced good comparative data with the flotation technique (<jats:italic>A. siro<\/jats:italic> r<jats:sup>2<\/jats:sup>=0.91, <jats:italic>Tyrophagus spp<\/jats:italic>. r<jats:sup>2<\/jats:sup>=0.99).<\/jats:p>","DOI":"10.1017\/s0007485308005634","type":"journal-article","created":{"date-parts":[[2008,2,18]],"date-time":"2008-02-18T06:19:44Z","timestamp":1203315584000},"page":"207-213","source":"Crossref","is-referenced-by-count":10,"title":["Rapid method for the detection of storage mites in cereals: feasibility of an ELISA based approach"],"prefix":"10.1017","volume":"98","author":[{"given":"J.A.","family":"Dunn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.B.","family":"Thind","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Danks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Chambers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2008,2,18]]},"reference":[{"key":"S0007485308005634_ref029","first-page":"366","volume-title":"A Biologist's Guide to Principlies and Techniques of Practical Biochemistry","author":"Wilson","year":"1986"},{"key":"S0007485308005634_ref028","first-page":"555","article-title":"Storage mite allergy is common in a farming population","volume":"15","author":"Van Hage-Hamsten","year":"1985","journal-title":"Clinical Immunology"},{"key":"S0007485308005634_ref025","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-004-2950-z"},{"key":"S0007485308005634_ref024","first-page":"113","article-title":"Determination of low levels of mite and insect contaminants on food and feed stuffs by a modified flotation method","volume":"83","author":"Thind","year":"2000","journal-title":"Journal of Association of Official Analytical Chemists"},{"key":"S0007485308005634_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/0022-474X(94)90267-4"},{"key":"S0007485308005634_ref022","unstructured":"Stengard Hansen L. , Herling C. & Danielson C. (1996) Densities of Lepidoglyphus destructor and levels of its major allergen Lep d 1 in grain flour. p. 569 in Proceedings of the XX International Congress of Entomology. August 25\u201331, Firenze, Italy."},{"key":"S0007485308005634_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90138-E"},{"key":"S0007485308005634_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/0022-474X(85)90058-X"},{"key":"S0007485308005634_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(89)90010-0"},{"key":"S0007485308005634_ref016","first-page":"711","article-title":"Polyclonal antibodies for detection of flour mite Acarus siro (Acari: Acaridida)","volume":"14","author":"Kudl\u00edkov\u00e1","year":"2004","journal-title":"Phytophaga"},{"key":"S0007485308005634_ref015","first-page":"711","volume-title":"Antibodies: A Laboratory Manual","author":"Harlow","year":"1988"},{"key":"S0007485308005634_ref013","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb02396.x"},{"key":"S0007485308005634_ref012","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.00922.x"},{"key":"S0007485308005634_ref011","doi-asserted-by":"publisher","DOI":"10.1080\/0954010021000096337"},{"key":"S0007485308005634_ref009","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1979.tb01547.x"},{"key":"S0007485308005634_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)80005-X"},{"key":"S0007485308005634_ref008","doi-asserted-by":"publisher","DOI":"10.1080\/09540109309354795"},{"key":"S0007485308005634_ref007","unstructured":"Chambers J. , Thind B.B. , Dunn J.A. & Pearson D.L. (1999b) The importance of storage mite allergens in occupational and domestic environments. pp. 559\u2013560. in Third International Conference on Urban Pests. Czech University of Agriculture, 19\u201322nd July, 1999, Prague."},{"key":"S0007485308005634_ref006","unstructured":"Chambers J. , Dunn J.A. & Thind B.B. (1999a) A rapid, sensitive, user-friendly method for detecting storage mites. HGCA Project Report No. 208, 15 pp."},{"key":"S0007485308005634_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0007485308005634_ref003","first-page":"363","volume-title":"Present and Future of Indoor Air Quality","author":"Bischoff","year":"1989"},{"key":"S0007485308005634_ref026","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010647519044"},{"key":"S0007485308005634_ref014","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1996.tb04602.x"},{"key":"S0007485308005634_ref010","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2338.2000.tb00922.x"},{"key":"S0007485308005634_ref027","first-page":"733","article-title":"Management of storage mites in production premises through early detection and monitoring using B T Mite Traps","volume":"14","author":"Thind","year":"2004","journal-title":"Phytophaga"},{"key":"S0007485308005634_ref001","volume-title":"Storage mites in foodstuffs. Food Surveillance Information Sheet 96, October 1996","year":"1996"},{"key":"S0007485308005634_ref019","doi-asserted-by":"publisher","DOI":"10.1159\/000237220"},{"key":"S0007485308005634_ref002","volume-title":"The Grain Storage Guide, second edition, 2003","year":"2003"},{"key":"S0007485308005634_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-474X(01)00006-6"}],"container-title":["Bulletin of Entomological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007485308005634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T20:06:12Z","timestamp":1556654772000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007485308005634\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2,18]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["S0007485308005634"],"URL":"https:\/\/doi.org\/10.1017\/s0007485308005634","relation":{},"ISSN":["0007-4853","1475-2670"],"issn-type":[{"value":"0007-4853","type":"print"},{"value":"1475-2670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2,18]]}}}