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Eucalyptus oil nanoemulsion was formulated in various ratios comprising of eucalyptus oil, tween 80 and water by ultrasonication. The stability of nanoemulsion was observed over a period of time and 1:2 ratios of eucalyptus oil (6%) and surfactant (12%) was found to be stable. The formulated eucalyptus oil nanoemulsion was characterized by transmission electron microscopy and dynamic light scattering. The nanoemulsion droplets were found to have a <jats:italic>Z<\/jats:italic>-average diameter of 9.4\u00a0nm and were spherical in shape. The larvicidal activity of eucalyptus oil nanoemulsion and bulk emulsion was tested and compared. Our nanoemulsion showed higher activity when compared to bulk emulsion. The histopathology of larvae-treated and untreated nanoemulsion was analyzed. Furthermore, biochemical assays were carried out to examine the effect of nanoemulsion on biochemical characteristics of larvae. The treated larval homogenate showed decrease in total protein content and a significant reduction in the levels of acetylcholinesterase. The levels of acid and alkaline phosphatase also showed reduction as compared to control larval homogenate.<\/jats:p>","DOI":"10.1017\/s0007485313000710","type":"journal-article","created":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T08:26:03Z","timestamp":1389255963000},"page":"393-402","source":"Crossref","is-referenced-by-count":223,"title":["Nanoemulsion of eucalyptus oil and its larvicidal activity against <i>Culex quinquefasciatus<\/i>"],"prefix":"10.1017","volume":"104","author":[{"given":"S.","family":"Sugumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.K.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.J.","family":"Nirmala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.K.","family":"Tyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mukherjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Chandrasekaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,1,9]]},"reference":[{"key":"S0007485313000710_ref38a","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.11.091"},{"key":"S0007485313000710_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2008.01.002"},{"key":"S0007485313000710_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9797(69)80012-3"},{"key":"S0007485313000710_ref37","first-page":"1342","article-title":"Potential larvicidal and pupacidal activities of herbal essential oils against Culex quinquefasciatus Say and Anopheles minimus (Theobald)","volume":"41","author":"Phasomkusolsil","year":"2010","journal-title":"The Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0007485313000710_ref12","doi-asserted-by":"publisher","DOI":"10.3201\/eid0404.980410"},{"key":"S0007485313000710_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.01.015"},{"key":"S0007485313000710_ref54","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2007.04.079"},{"key":"S0007485313000710_ref52","first-page":"243","article-title":"Evaluation of eucalyptus-based repellent against Anopheles spp. in Tanzania","volume":"12","author":"Trigg","year":"1996","journal-title":"Journal of the American Mosquito Control Association"},{"key":"S0007485313000710_ref7","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.4.726"},{"key":"S0007485313000710_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(81)71086-3"},{"key":"S0007485313000710_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0022-0981(78)90067-9"},{"key":"S0007485313000710_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2011.07.014"},{"key":"S0007485313000710_ref13","volume-title":"Field Guide to the Eucalyptus","volume":"1","author":"Brooker","year":"1990"},{"key":"S0007485313000710_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.04.066"},{"key":"S0007485313000710_ref47","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-012-1161-0"},{"key":"S0007485313000710_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0007485313000710_ref17","unstructured":"Global Health (2010). 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