{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:49:02Z","timestamp":1780494542041,"version":"3.54.1"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Bull. Entomol. Res."],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The gene encoding juvenile hormone response (Kr\u00fcppel homolog1, <jats:italic>Kr-hl<\/jats:italic>) in <jats:italic>Coccinella septempunctata<\/jats:italic> was investigated by cloning and analysing expression profiles in different developmental stages and tissues by quantitative real-time polymerase chain reaction (PCR). <jats:italic>C. septempunctata Kr-hl<\/jats:italic> (<jats:italic>CsKr-hl<\/jats:italic>) encoded a 1338 bp open reading frame (ORF) with a predicted protein product of 445 amino acids; the latter showed high similarity to orthologs in other species and contained eight highly-conserved Zn-finger motifs for DNA-binding. <jats:italic>CsKr-hl<\/jats:italic> was expressed in different developmental stages of <jats:italic>C. septempunctata<\/jats:italic>. The expression levels of <jats:italic>CsKr-hl<\/jats:italic> in eggs, 2nd, 3rd, 4th instar larvae, and pupa were 3.31, 2.30, 7.09, 0.58, and 7.48 times the number of 1st instar larvae, respectively. <jats:italic>CsKr-hl<\/jats:italic> expression levels in female adults gradually increased at 25\u201330 days and were significantly higher than expression at 1\u201320 days. <jats:italic>CsKr-hl<\/jats:italic> expression in 20\u201330 days-old male adults was significantly higher than males aged 1\u201315 days. <jats:italic>CsKr-hl<\/jats:italic> expression levels in heads of male and female adults were significantly higher than expression levels in the thorax, adipose, and reproductive system. Interestingly, <jats:italic>CsKr-hl<\/jats:italic> expression levels in the adipose and reproductive system of female adults were significantly higher than in adult male corresponding organs, which suggest that <jats:italic>CsKr-hl<\/jats:italic> plays an important role in regulating reproductive development in <jats:italic>C. septempunctata<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0007485323000652","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T08:31:01Z","timestamp":1704443461000},"page":"99-106","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Cloning and spatio-temporal expression of <i>CsKr-h1<\/i> encoding the juvenile hormone response gene in <i>Coccinella septempunctata<\/i> 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