{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:09:44Z","timestamp":1790327384023,"version":"4.1.0"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371883"],"award-info":[{"award-number":["32371883"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Bull. Entomol. Res."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Micromelalopha sieversi<\/jats:italic>\n                    (Staudinger) is a major forest pest causing severe damage to poplar plantations in China. However, the molecular mechanisms underlying its adaptation to plant secondary metabolites remain largely unclear. In this study, a P450 gene designated\n                    <jats:italic>MsCYP9A319<\/jats:italic>\n                    was screened from tannic acid-induced transcriptomes of\n                    <jats:italic>M. sieversi<\/jats:italic>\n                    and cloned. Sequence analysis revealed that\n                    <jats:italic>MsCYP9A319<\/jats:italic>\n                    belongs to the CYP9 family and contains typical conserved domains of cytochrome P450 proteins. Real-time quantitative polymerase chain reaction (RT-qPCR) assays showed that\n                    <jats:italic>MsCYP9A319<\/jats:italic>\n                    was highly expressed in the midgut and head of larvae, with the highest expression level in the 3rd instar, and was significantly induced by 0.1\u00a0mg\/mL tannic acid. In addition, we performed molecular docking experiments, and the results indicated that MsCYP9A319 exhibited strong binding affinity to tannic acid, providing further evidence that this protein can specifically recognise tannic acid as its substrate. Furthermore, RNAi-mediated silencing of\n                    <jats:italic>MsCYP9A319<\/jats:italic>\n                    significantly decreased larval tolerance to tannic acid. These results indicate that\n                    <jats:italic>MsCYP9A319<\/jats:italic>\n                    participates in the response of\n                    <jats:italic>M. sieversi<\/jats:italic>\n                    to tannic acid stress, and provide a theoretical basis for elucidating host adaptation mechanisms and screening target genes for green pest control.\n                  <\/jats:p>","DOI":"10.1017\/s0007485326101163","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:31:07Z","timestamp":1790325067000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Functional study of the\n                    <i>MsCYP9A319<\/i>\n                    in\n                    <i>Micromelalopha sieversi<\/i>\n                    in response to tannic acid stress"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7859-8917","authenticated-orcid":false,"given":"Fenglin","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03m96p165","id-type":"ROR","asserted-by":"publisher"}],"name":"Nanjing Forestry University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Ren","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03m96p165","id-type":"ROR","asserted-by":"publisher"}],"name":"Nanjing Forestry University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuoai","family":"Zeng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03m96p165","id-type":"ROR","asserted-by":"publisher"}],"name":"Nanjing Forestry University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3162-6084","authenticated-orcid":false,"given":"Fang","family":"Tang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03m96p165","id-type":"ROR","asserted-by":"publisher"}],"name":"Nanjing Forestry University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"S0007485326101163_ref8","doi-asserted-by":"publisher","DOI":"10.18474\/JES20-48"},{"key":"S0007485326101163_ref22","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-070621-061328"},{"key":"S0007485326101163_ref23","doi-asserted-by":"publisher","DOI":"10.18474\/JES18-71"},{"key":"S0007485326101163_ref29","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160922606"},{"key":"S0007485326101163_ref27","first-page":"41","article-title":"Main poplar pests and their control in Northern Jiangsu","volume":"3","author":"Wang","year":"2001","journal-title":"Journal of Shandong Forestry Science and Technology"},{"key":"S0007485326101163_ref28","unstructured":"Wang, M (2020) Adaptation of Hyphantria cunea to plant secondary metabolites [Master\u2019s dissertation]. Northeast Forestry University."},{"key":"S0007485326101163_ref25","doi-asserted-by":"publisher","DOI":"10.3390\/insects11060339"},{"key":"S0007485326101163_ref34","first-page":"10","article-title":"Studies on the natural population dynamics of Micromelalopha sieversi","volume":"1999","author":"Zhang","year":"1999","journal-title":"Shaanxi Forest Science and Technology"},{"key":"S0007485326101163_ref11","first-page":"115","article-title":"A study on the occurrence rule of Micromelalopha troglodyta","volume":"30","author":"Guo","year":"2006","journal-title":"Journal of Nanjing Forestry University (Natural Sciences Edition)"},{"key":"S0007485326101163_ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.44.1.507"},{"key":"S0007485326101163_ref21","first-page":"54","article-title":"Control effect of Micromelalopha sieversi in Laoting County achieved results","volume":"2021","author":"Mu","year":"2021","journal-title":"Land Greening"},{"key":"S0007485326101163_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.08.034"},{"key":"S0007485326101163_ref7","unstructured":"Fan, LP (2014) Study on the reproductive behaviour and host selection mechanism of Micromelalopha sieversi [Doctoral dissertation]. Chinese Academy of Forestry."},{"key":"S0007485326101163_ref17","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"S0007485326101163_ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s12600-016-0550-1"},{"key":"S0007485326101163_ref37","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/iev100"},{"key":"S0007485326101163_ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2012.05548.x"},{"key":"S0007485326101163_ref32","first-page":"20","article-title":"Study on biological characteristics of Micromelalopha sieversi","volume":"1999","author":"Yang","year":"1999","journal-title":"Shaanxi Forest Science and Technology"},{"key":"S0007485326101163_ref16","first-page":"32","article-title":"Inhibitory effect of tannic acid on growth, development and phenoloxidase activity of Spodoptera exigua larvae","volume":"19","author":"Liu","year":"2010","journal-title":"Journal of Plant Resources and Environment"},{"key":"S0007485326101163_ref4","first-page":"32","article-title":"Relationship between trunk tannin content of poplar cultivars in Heilongjiang Province and infestation by Xylotrechus rusticus (Coleoptera: Cerambycidae)","volume":"34","author":"Cheng","year":"2006","journal-title":"Journal of Northeast Forestry University"},{"key":"S0007485326101163_ref18","first-page":"48","article-title":"Damage characteristics and control methods of Clostera anachoreta and Micromelalopha sieversi","volume":"25","author":"Lu","year":"2022","journal-title":"Special Economic Animals and Plants"},{"key":"S0007485326101163_ref24","doi-asserted-by":"publisher","DOI":"10.3390\/insects13100872"},{"key":"S0007485326101163_ref1","volume-title":"Economic Insect Fauna of China (Fasc. 16) (Lepidoptera: Notodontidae)","author":"Cai","year":"1981"},{"key":"S0007485326101163_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485315000760"},{"key":"S0007485326101163_ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0221646"},{"key":"S0007485326101163_ref35","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485323000718"},{"key":"S0007485326101163_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101515"},{"key":"S0007485326101163_ref36","first-page":"130","article-title":"Cloning and detoxification function of the CYP9A134 gene in the Mythimna separata","volume":"39","author":"Zhang","year":"2023","journal-title":"Chinese Journal of Biological Controlled"},{"key":"S0007485326101163_ref12","first-page":"109","article-title":"Occurrence and integrated control techniques of Micromelalopha sieversi on poplar","volume":"2014","author":"Li","year":"2014","journal-title":"Modern Horticulture"},{"key":"S0007485326101163_ref10","first-page":"142","article-title":"Occurrence causes and control measures of Micromelalopha sieversi on poplar","volume":"30","author":"Gu","year":"2014","journal-title":"Chinese Horticulture Abstracts"},{"key":"S0007485326101163_ref31","first-page":"74","article-title":"Study on biological characteristics and control of Micromelalopha sieversi","volume":"2010","author":"Wu","year":"2010","journal-title":"Anhui Forestry Science and Technology"},{"key":"S0007485326101163_ref2","first-page":"1126","article-title":"Metabolic adaptation mechanisms of insects to plant secondary substances and their significance for insecticide resistance","volume":"58","author":"Chen","year":"2015","journal-title":"Acta Entomologica Sinica"},{"key":"S0007485326101163_ref19","first-page":"708","article-title":"Expression patterns of P450 genes in insecticide resistant\/detoxified populations of two Trichogramma species","volume":"62","author":"Lu","year":"2025","journal-title":"Chinese Journal of Applied Entomology"},{"key":"S0007485326101163_ref14","first-page":"9","article-title":"Effects of oviposition behaviour of Micromelalopha sieversi on nutrient and resistances in leaves of two clones of Populus Section Aigeiros","volume":"32","author":"Liu","year":"2019","journal-title":"Forest Research"},{"key":"S0007485326101163_ref30","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c05423"},{"key":"S0007485326101163_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2011.04.009"},{"key":"S0007485326101163_ref3","first-page":"941","article-title":"Cloning and expression analysis of cytochrome P450 genes CYP332A19 and CYP337B19 in the codling moth, Cydia pomonella","volume":"63","author":"Chen","year":"2020","journal-title":"Acta Entomologica Sinica"},{"key":"S0007485326101163_ref15","unstructured":"Liu, M (2023) Screening and Functional Analysis of P450 Genes in Response to Abamectin and Chlorantraniliprole Complex Stress in Tuta absoluta [Master\u2019s dissertation]. Guizhou University."}],"container-title":["Bulletin of Entomological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007485326101163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:31:11Z","timestamp":1790325071000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007485326101163\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":38,"alternative-id":["S0007485326101163"],"URL":"https:\/\/doi.org\/10.1017\/s0007485326101163","relation":{},"ISSN":["0007-4853","1475-2670"],"issn-type":[{"value":"0007-4853","type":"print"},{"value":"1475-2670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. 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