{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:37:57Z","timestamp":1789486677344,"version":"build-2803163510"},"reference-count":77,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Bull. Entomol. Res."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The diamondback moth,\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    (L.) (Lepidoptera: Plutellidae), is a major pest of cruciferous crops and has developed high levels of resistance to many widely used insecticides. Neuropeptides are involved in regulating essential insect biological processes, which could be considered potential target genes for pest control. In this study, a total of 45 neuropeptide precursor genes were identified in the genome of\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , including two adipokinetic hormone genes (\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    and\n                    <jats:italic>PxAKH2<\/jats:italic>\n                    ). The qPCR analysis showed that\n                    <jats:italic>PxAKH<\/jats:italic>\n                    genes were highly expressed in third- and fourth-instar larvae and exhibited high expression levels in the fat body, head, and gut of\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    larvae. Additionally, short-term starvation stimulated food consumption in\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , which was consistent with the significant induction of\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    within 6\u00a0h. Subsequently, RNA interference (RNAi) was utilised to investigate the functions of\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    in\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , and the injection of ds\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    significantly suppressed target gene expression, achieving a maximum silencing efficiency of 73.03%. Notably, knockdown of\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    increased triacylglycerol levels from 23.67\u00a0\u00b1\u00a00.56\u00a0mg\/g in the ds\n                    <jats:italic>GFP<\/jats:italic>\n                    control to 25.84\u00a0\u00b1\u00a00.64\u00a0mg\/g and enhanced lipid storage, while markedly reducing food intake from 221.33\u00a0\u00b1\u00a09.10\u00a0mm\n                    <jats:sup>2<\/jats:sup>\n                    to 123.33\u00a0\u00b1\u00a018.80\u00a0mm\n                    <jats:sup>2<\/jats:sup>\n                    in\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    larvae. In summary, these findings demonstrate that\n                    <jats:italic>PxAKH1<\/jats:italic>\n                    plays a critical role in regulating lipid mobilisation and food intake in\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , indicating its potential as an RNAi-based target for pest control.\n                  <\/jats:p>","DOI":"10.1017\/s0007485326101254","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:11:51Z","timestamp":1789477911000},"page":"1-14","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification of neuropeptides from\n                    <i>Plutella xylostella<\/i>\n                    (Lepidoptera: Plutellidae) and elucidating the essential roles of adipokinetic hormone in lipid mobilisation and food intake"],"prefix":"10.1017","author":[{"given":"Mei","family":"Fang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hai","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Ni","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Xuan","family":"Xu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Xian","family":"Gan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Guo","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02z2d6373","id-type":"ROR","asserted-by":"publisher"}],"name":"Yunnan Academy of Agricultural Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-9142","authenticated-orcid":false,"given":"Qiu-Li","family":"Hou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Yangzhou University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"S0007485326101254_ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125411"},{"key":"S0007485326101254_ref74","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8459"},{"key":"S0007485326101254_ref55","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-023-29271-5"},{"key":"S0007485326101254_ref71","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.05.001"},{"key":"S0007485326101254_ref13","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.115.178897"},{"key":"S0007485326101254_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.141929"},{"key":"S0007485326101254_ref31","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2015.00151"},{"key":"S0007485326101254_ref5","first-page":"142","article-title":"Adipokinetic hormone exerts its anti-oxidative effects using a conserved signal-transduction mechanism involving both PKC and cAMP by mobilizing extra- and intracellular Ca2+ stores","volume":"158","author":"Bedn\u00e1\u0159ov\u00e1","year":"2013","journal-title":"Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology"},{"key":"S0007485326101254_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2023.101119"},{"key":"S0007485326101254_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.09.006"},{"key":"S0007485326101254_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jia.2024.03.019"},{"key":"S0007485326101254_ref35","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12751"},{"key":"S0007485326101254_ref56","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8070"},{"key":"S0007485326101254_ref76","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23169019"},{"key":"S0007485326101254_ref57","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0007485326101254_ref11","doi-asserted-by":"publisher","DOI":"10.1002\/ps.755"},{"key":"S0007485326101254_ref33","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07822-4"},{"key":"S0007485326101254_ref3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085356"},{"key":"S0007485326101254_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.05.014"},{"key":"S0007485326101254_ref12","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21611"},{"key":"S0007485326101254_ref44","doi-asserted-by":"publisher","DOI":"10.3390\/insects16040407"},{"key":"S0007485326101254_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.11.010"},{"key":"S0007485326101254_ref32","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.167.1.311"},{"key":"S0007485326101254_ref9","first-page":"3217","article-title":"Role of dsRNA-based insecticides in agriculture: Current scenario and future prospects","volume":"93","author":"Das","year":"2024","journal-title":"Phyton-International Journal of Experimental Botany"},{"key":"S0007485326101254_ref4","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-13-71"},{"key":"S0007485326101254_ref41","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"S0007485326101254_ref27","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124128"},{"key":"S0007485326101254_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2021.104339"},{"key":"S0007485326101254_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2025.104754"},{"key":"S0007485326101254_ref60","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7144"},{"key":"S0007485326101254_ref68","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10340-022-01511-6","article-title":"Transcriptome analysis of neuropeptides in the beneficial insect lacewing (Chrysoperla carnea) identifies kinins as a selective pesticide target: A biostable kinin analogue with activity against the peach potato aphid Myzus persicae","volume":"96","author":"Yan","year":"2023","journal-title":"Journal of Pest Science"},{"key":"S0007485326101254_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2024.101300"},{"key":"S0007485326101254_ref72","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.15693"},{"key":"S0007485326101254_ref59","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2010.01.015"},{"key":"S0007485326101254_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2019.103257"},{"key":"S0007485326101254_ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2024.1464989"},{"key":"S0007485326101254_ref50","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.11.008"},{"key":"S0007485326101254_ref29","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12861"},{"key":"S0007485326101254_ref58","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2013.07.027"},{"key":"S0007485326101254_ref53","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-031616-035500"},{"key":"S0007485326101254_ref54","doi-asserted-by":"publisher","DOI":"10.3390\/cells9122667"},{"key":"S0007485326101254_ref67","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21625"},{"key":"S0007485326101254_ref34","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6714008"},{"key":"S0007485326101254_ref73","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2016.06.010"},{"key":"S0007485326101254_ref75","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/imb.12920","article-title":"Rhythmic change of adipokinetic hormones diurnally regulates locust vitellogenesis and egg development","volume":"33","author":"Zheng","year":"2024","journal-title":"Insect Molecular Biology"},{"key":"S0007485326101254_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00175-1"},{"key":"S0007485326101254_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/1748-5967.12270"},{"key":"S0007485326101254_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105536"},{"key":"S0007485326101254_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2017.10.009"},{"key":"S0007485326101254_ref77","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5569"},{"key":"S0007485326101254_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.10.156"},{"key":"S0007485326101254_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.01.011"},{"key":"S0007485326101254_ref61","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8598"},{"key":"S0007485326101254_ref42","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12535"},{"key":"S0007485326101254_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2008.04.009"},{"key":"S0007485326101254_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2022.03.034"},{"key":"S0007485326101254_ref45","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13264"},{"key":"S0007485326101254_ref24","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53103"},{"key":"S0007485326101254_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(69)90259-5"},{"key":"S0007485326101254_ref69","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0130905"},{"key":"S0007485326101254_ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050137"},{"key":"S0007485326101254_ref47","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.04.010"},{"key":"S0007485326101254_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.06.013"},{"key":"S0007485326101254_ref38","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010715-023622"},{"key":"S0007485326101254_ref43","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01730"},{"key":"S0007485326101254_ref64","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c01873"},{"key":"S0007485326101254_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108179"},{"key":"S0007485326101254_ref30","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12978"},{"key":"S0007485326101254_ref52","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-97851-x"},{"key":"S0007485326101254_ref6","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12314"},{"key":"S0007485326101254_ref39","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c09458"},{"key":"S0007485326101254_ref63","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c03182"},{"key":"S0007485326101254_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485322000141"},{"key":"S0007485326101254_ref22","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c09279"},{"key":"S0007485326101254_ref65","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2025.106303"},{"key":"S0007485326101254_ref66","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2023.102070"},{"key":"S0007485326101254_ref70","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c01465"}],"container-title":["Bulletin of Entomological Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007485326101254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:11:53Z","timestamp":1789477913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007485326101254\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":77,"alternative-id":["S0007485326101254"],"URL":"https:\/\/doi.org\/10.1017\/s0007485326101254","relation":{},"ISSN":["0007-4853","1475-2670"],"issn-type":[{"value":"0007-4853","type":"print"},{"value":"1475-2670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}