{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:59:16Z","timestamp":1790981956701,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genet. Res."],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p>\n                    Polymerase chain reaction (PCR)-based genotyping of oocysts dissected from mosquito midguts \nhas previously been used to investigate overall levels of inbreeding within malaria parasite \npopulations. We present a re-analysis of the population structure of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    malaria using diploid genotypes at three antigen-encoding loci in 118 oocysts dissected from 34 \nmosquitoes. We use these data to ask whether mating is occurring at random within the mosquito \nmidgut, as is generally assumed. We observe a highly significant deficit of heterozygous oocysts\n                    <jats:italic>within mosquitoes<\/jats:italic>\n                    at all three loci, suggesting that fusion of gametes occurs non-randomly in the \nmosquito gut. A variety of biological explanations, such as interrupted feeding of mosquitoes, \npositive assortative mating and outcrossing depression, could account for this observation. \nHowever, an alternative artefactual explanation \u2013 the presence of non-amplifying or null alleles \u2013 \ncan account for the observed data equally well, without the need to invoke non-random mating. \nTo evaluate this explanation further, we estimate the frequencies of null alleles within the oocyst \npopulation using maximum likelihood, by making the assumption that non-amplifying oocysts at \nany of the three loci are homozygous for null alleles. Observed levels of visible heterozygotes fit \nclosely with those expected under random mating when non-amplifying oocysts are accounted for. \nOther lines of evidence also support the artefactual explanation. Overall inbreeding coefficients \nhave been recalculated in the light of this analysis, and may be considerably lower than those \nestimated previously. In conclusion, we suggest that the deficit of heterozygotes observed is \nunlikely to indicate non-random mating within the mosquito gut and is better explained by \nmisscoring of heterozygotes as homozygotes.\n                  <\/jats:p>","DOI":"10.1017\/s0016672300004481","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:23:20Z","timestamp":1027761800000},"page":"285-296","source":"Crossref","is-referenced-by-count":36,"title":["Do malaria parasites mate non-randomly in the mosquito midgut?"],"prefix":"10.1155","volume":"75","author":[{"given":"T. J. C.","family":"ANDERSON","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. E. L.","family":"PAUL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"DONNELLY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. P.","family":"DAY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2000,6,1]]},"container-title":["Genetical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0016672300004481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T17:45:50Z","timestamp":1609436750000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0016672300004481\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["S0016672300004481"],"URL":"https:\/\/doi.org\/10.1017\/s0016672300004481","relation":{},"ISSN":["0016-6723","1469-5073"],"issn-type":[{"value":"0016-6723","type":"print"},{"value":"1469-5073","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6]]}}}