{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T12:22:43Z","timestamp":1793362963732,"version":"4.5.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2000,10,19]],"date-time":"2000-10-19T00:00:00Z","timestamp":971913600000},"content-version":"unspecified","delay-in-days":79,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>We have investigated the effects of adding a range of mineral salts and \ncalcium-chelating agents on the distribution of casein and minerals between the non-pelleted and pelleted phases of milk obtained upon centrifugation at 78000 <jats:bold><jats:italic>g<\/jats:italic><\/jats:bold> for \n90 min. Adding CaCl<jats:sub>2<\/jats:sub> or mixtures of NaH<jats:sub>2<\/jats:sub>PO% and Na<jats:sub>2<\/jats:sub>HPO% to reconstituted skim \nmilk (90 g milk solids\/kg) at pH 6\u00b765 increased both pelleted casein and pelleted \ncalcium phosphate. Opposite effects were obtained by adding citrate or EDTA. The \nchange in pelleted calcium phosphate was not simply related to casein release from \nthe micelle. Upon adding 5 mmol EDTA\/kg milk, 20% of the pelleted Ca, 22% of \nthe pelleted phosphate and 5% of the micellar casein were removed. Increasing the \nconcentration of EDTA to 10 mmol\/kg milk decreased the pelleted Ca by 44% and \nthe pelleted phosphate by 46%, and caused 30%of the micellar casein to be released. \nThe effects of adding phosphate, citrate or EDTA at pH 6\u00b765, followed by the \naddition of CaCl<jats:sub>2<\/jats:sub>, demonstrated the reversibility of the dissolution and formation of \nthe micellar calcium phosphate. There were limits to this reversibility that were \nrelated to the amount of colloidal calcium phosphate removed from the casein \nmicelles. Adding CaCl<jats:sub>2<\/jats:sub> to milk containing [ges     ] 20 mmol EDTA or [ges     ] 30 mmol \ncitrate\/kg milk did not result in complete reformation of casein micelles. Light-scattering experiments confirmed that the dissolution of moderate amounts of \ncolloidal calcium phosphate had little effect on micellar size and were reversible, \nwhile the dissolution of larger amounts of colloidal calcium phosphate resulted in \nlarge reductions in micellar size and was irreversible.<\/jats:p>","DOI":"10.1017\/s0022029900004271","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:45:41Z","timestamp":1027763141000},"page":"361-370","source":"Crossref","is-referenced-by-count":141,"title":["Mineral and casein equilibria in milk: effects of added salts and calcium-chelating agents"],"prefix":"10.1017","volume":"67","author":[{"given":"PUNSANDANI","family":"UDABAGE","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IAN R.","family":"McKINNON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARY-ANN","family":"AUGUSTIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2000,10,19]]},"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029900004271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T19:34:13Z","timestamp":1554492853000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029900004271\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["S0022029900004271"],"URL":"https:\/\/doi.org\/10.1017\/s0022029900004271","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,8]]}}}