{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:05:55Z","timestamp":1791918355919,"version":"4.3.4"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"unspecified","delay-in-days":8521,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[1986,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Conditions necessary for the determination of crude extracellular lipase activity from<jats:italic>Pseudomonas fluorescens<\/jats:italic>B52 using \u03b2-naphthyl caprylate (\u03b2-NC), an 8-carbon ester, as the substrate were examined. Maximum enzyme synthesis occurred at 20 \u00b0C in pyruvate mineral salts medium containing 1 mM-CaCl<jats:sub>2<\/jats:sub>. Bile salts were necessary for enzyme activity; 6 mM-Na taurocholate or Na deoxycholate gave maximum activity but the latter compound was inhibitory at higher concentrations. Activity was optimal in N-Tris[hydroxymethyl]methyl-2-aminoethane sulphonic acid buffer pH 8\u00b70 at 40 \u00b0C. A comparison of \u03b2-NC with \u03b2-naphthyl butyrate (a 4-carbon ester) and \u03b2-naphthyl myristate (a 14-carbon ester) showed that \u03b2-NC was the best substrate;<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>values of 0\u00b70415, 0\u00b7141 and 0\u00b7200 mM and<jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub>values of 67\u00b72, 20\u00b71 and 5\u00b728 \u03bcmol ml<jats:sup>-1<\/jats:sup>h<jats:sup>-1<\/jats:sup>were obtained for those substrates respectively. The enzyme was inhibited 50% in its activity against \u03b2-NC by 0\u00b7009 mM-EDTA, 0\u20130007% (w\/v) mixed alkyltrimethylammonium bromide and 0\u00b700275% (w\/v) Triton X-100. The biochemical properties determined using \u03b2-NC as substrate are consistent with those reported for the lipases of other strains of<jats:italic>Ps. fluorescens<\/jats:italic>using natural substrates.<\/jats:p>","DOI":"10.1017\/s0022029900024717","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T10:45:59Z","timestamp":1244198759000},"page":"117-127","source":"Crossref","is-referenced-by-count":21,"title":["A rapid colorimetric assay for the extracellular lipase of<i>Pseudomonas fluorescens<\/i>B52 using \u03b2-naphthyl caprylate"],"prefix":"10.1017","volume":"53","author":[{"given":"Robin C.","family":"McKellar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,6,1]]},"reference":[{"key":"S0022029900024717_ref006","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900021117"},{"key":"S0022029900024717_ref018","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(80)82913-4"},{"key":"S0022029900024717_ref020","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900023384"},{"key":"S0022029900024717_ref010","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1981.tb00894.x"},{"key":"S0022029900024717_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900017611"},{"key":"S0022029900024717_ref009","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900032544"},{"key":"S0022029900024717_ref005","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-45.2.172"},{"key":"S0022029900024717_ref001","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(81)82796-8"},{"key":"S0022029900024717_ref016","first-page":"1051","article-title":"The colorimetrie determination of urinary lipase","volume":"230","author":"Nachlas","year":"1958","journal-title":"Journal of Biological Chemistry"},{"key":"S0022029900024717_ref002","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(81)82581-7"},{"key":"S0022029900024717_ref003","doi-asserted-by":"publisher","DOI":"10.1021\/jf00121a001"},{"key":"S0022029900024717_ref004","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(84)81492-7"},{"key":"S0022029900024717_ref007","first-page":"10","article-title":"Thermal activation and inactivation of exocellular lipases of some Gram-negative bacteria common in milk","volume":"28","author":"Driessen","year":"1974","journal-title":"Netherlands Milk and Dairy Journal"},{"key":"S0022029900024717_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900020057"},{"key":"S0022029900024717_ref014","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1128\/AEM.47.6.1224-1227.1984","article-title":"Synthesis of extracellular proteinase by Pseudomonas fluorescens under conditions of limiting carbon, nitrogen, and phosphate","volume":"47","author":"McKellar","year":"1984","journal-title":"Applied and Environmental Microbiology"},{"key":"S0022029900024717_ref015","first-page":"87","article-title":"Heat resistant enzymes in UHT milk and their influence on sensoric changes during uncooled storage","volume":"36","author":"Mottar","year":"1981","journal-title":"Milchwissenschaft"},{"key":"S0022029900024717_ref017","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1980.tb05124.x"},{"key":"S0022029900024717_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900016046"},{"key":"S0022029900024717_ref019","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029900032726"},{"key":"S0022029900024717_ref008","first-page":"97","article-title":"Factors influencing lipolysis by skim milk cultures of some psychrotrophic microorganisms","volume":"38","author":"Fitz-Gerald","year":"1983","journal-title":"Australian Journal of Dairy Technology"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029900024717","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T05:26:14Z","timestamp":1589865974000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029900024717\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,2]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,2]]}},"alternative-id":["S0022029900024717"],"URL":"https:\/\/doi.org\/10.1017\/s0022029900024717","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,2]]}}}