{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T19:32:19Z","timestamp":1775590339440,"version":"3.50.1"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"unspecified","delay-in-days":6422,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[1991,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Lactobacilli, often used as effectors of host functions, could play an important role in maintaining human health by controlling other intestinal microorganisms capable of producing harmful effects. Using an experimental model, we studied the effect of different oral doses of<jats:italic>Lactobacillus casei<\/jats:italic>on the secretory IgA response and the protective capacity of the microorganism in preventing intestinal infections. The optimization of the protective dose of<jats:italic>Lb. casei<\/jats:italic>by previous feeding and the use of the lactobacillus as an immunological way to control enteric infections were investigated. We found that conventional mice were protected against infection with<jats:italic>Salmonella typhimurium<\/jats:italic>and<jats:italic>Escherichia coli<\/jats:italic>by previous feeding for 2 consecutive days with a daily<jats:italic>Lb. casei<\/jats:italic>dose of 1\u00b72 \u00d7 10<jats:sup>9<\/jats:sup>cfu\/mouse. Previous feeding for 7 d proved less effective, and feeding for 5 d afforded no protection at all. We were also able to demonstrate that the protective effect of<jats:italic>Lb. casei<\/jats:italic>against<jats:italic>Sal. typhimurium<\/jats:italic>and<jats:italic>Esch. coli<\/jats:italic>was connected mainly with the high level of IgA antipathogen antibodies present in intestinal secretions. \u03b2-Glucuronidase (EC 3.2.1.31) and \u03b2-galactosidase (EC 3.2.1.23) activities, measured both in the intestinal fluid and histological samples, showed a marked increase in intestinal inflammatory response on day 5 of feeding. These results show that<jats:italic>Lb. casei<\/jats:italic>plays an important role in the prevention of enteric infections, a low dose being enough for protection against intestinal infections by increasing IgA secretion into the intestinal lumen, thus providing adequate defences for the mucosal surface. A previously administered dose of this magnitude could therefore be used as an oral adjuvant in preventing enteric infections.<\/jats:p>","DOI":"10.1017\/s0022029900030090","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T10:29:37Z","timestamp":1244197777000},"page":"485-496","source":"Crossref","is-referenced-by-count":120,"title":["Immunoadjuvant activity of oral<i>Lactobacillus casei<\/i>: influence of dose on the secretory immune response and protective capacity in intestinal infections"],"prefix":"10.1017","volume":"58","author":[{"given":"Gabriela","family":"Perdig\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A\u00edda Pesce","family":"de Ruiz Holgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,6,1]]},"reference":[{"key":"S0022029900030090_ref028","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1542\/peds.57.6.901","article-title":"Host defense mechanisms in the gastrointestinal tract","volume":"57","author":"Walker","year":"1976","journal-title":"Pediatrics"},{"key":"S0022029900030090_ref027","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.04.040186.002133"},{"key":"S0022029900030090_ref026","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.2460\/ajvr.1982.43.12.2227","article-title":"Effect of Streptococcus faecium C-68 in control of Escherichia coli-induced diarrhea in gnotobiotic pigs","volume":"43","author":"Underdahl","year":"1982","journal-title":"American Journal of Veterinary Research"},{"key":"S0022029900030090_ref025","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1128\/IAI.53.3.511-516.1986","article-title":"Adjuvant activity of 6-0-acyl-muramyldipeptides to enhance primary cellular and humoral responses in guinea pigs: adaptability to various vehicles and pyrogenicity","volume":"53","author":"Tsujimoto","year":"1986","journal-title":"Infection and Immunity"},{"key":"S0022029900030090_ref024","doi-asserted-by":"publisher","DOI":"10.1038\/306184a0"},{"key":"S0022029900030090_ref023","volume-title":"Manual of Clinical Immunology","author":"Stossel","year":"1980"},{"key":"S0022029900030090_ref021","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1128\/IAI.56.2.387-394.1988","article-title":"Oral immunization of rabbits with enterotoxigenic Escherichia coli protects against intraintestinal challenge","volume":"56","author":"Sack","year":"1988","journal-title":"Infection and Immunity"},{"key":"S0022029900030090_ref020","first-page":"17","article-title":"Systemic augmentation of the immune response in mice by feeding fermented milks with Lactobacillus casei and Lactobacillus acidophilus","volume":"63","author":"Perdigon","year":"1988","journal-title":"Immunology"},{"key":"S0022029900030090_ref018","first-page":"344","article-title":"Lactobacilli administered orally induce release of enzymes from peritoneal macrophages in mice","volume":"41","author":"Perdigon","year":"1986","journal-title":"Milchwissenschaft"},{"key":"S0022029900030090_ref017","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1128\/AEM.55.2.279-283.1989","article-title":"Colonization of the porcine gastrointestinal tract by lactobacilli","volume":"55","author":"Pedersen","year":"1989","journal-title":"Applied and Environmental Microbiology"},{"key":"S0022029900030090_ref016","first-page":"91","article-title":"Augmentation of host resistance to Listeria monocytogenes infection by Lactobacillus casei","volume":"17","author":"Nomoto","year":"1985","journal-title":"Journal of Clinical and Laboratory Immunology"},{"key":"S0022029900030090_ref015","doi-asserted-by":"crossref","first-page":"903","DOI":"10.4049\/jimmunol.130.2.903","article-title":"Natural and antibody-dependent cell-mediated activity against Salmonella typhimurium by peripheral and intestinal lymphoid cells in mice","volume":"130","author":"Nencioni","year":"1983","journal-title":"Journal of Immunology"},{"key":"S0022029900030090_ref014","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1128\/IAI.46.1.22-24.1984","article-title":"Cytotoxicity of leukocytes from normal and Shigella-susceptible (opium-treated) guinea pigs against virulent Shigella sonnei","volume":"46","author":"Morgan","year":"1984","journal-title":"Infection and Immunity"},{"key":"S0022029900030090_ref013","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/IAI.48.2.480-485.1985","article-title":"Protective effect of Lactobacillus casei on Pseudomonas aeruginosa infection in mice","volume":"48","author":"Miake","year":"1985","journal-title":"Infection and Immunity"},{"key":"S0022029900030090_ref012","first-page":"1547","article-title":"Immune response to a live model oral vaccine against enteropathogenic E. coli: E. coli HB 101 (strain M5) expressing the AF\/RI pilus","volume":"90","author":"McQueen","year":"1986","journal-title":"Gastroenterology"},{"key":"S0022029900030090_ref011","first-page":"401","article-title":"Immune components of the intestinal mucosae of ageing and protein deficient mice","volume":"43","author":"Lim","year":"1981","journal-title":"Immunology"},{"key":"S0022029900030090_ref008","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4049\/jimmunol.109.1.129","article-title":"Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes","volume":"109","author":"Engvall","year":"1972","journal-title":"Journal of Immunology"},{"key":"S0022029900030090_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90019-6"},{"key":"S0022029900030090_ref006","doi-asserted-by":"publisher","DOI":"10.1042\/bj0710318"},{"key":"S0022029900030090_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-3369-2_52"},{"key":"S0022029900030090_ref002","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.6.1260"},{"key":"S0022029900030090_ref001","first-page":"289","volume-title":"Recent Advances in Mucosal Immunity","author":"Arnaud-Battandier","year":"1982"},{"key":"S0022029900030090_ref010","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.6.1661"},{"key":"S0022029900030090_ref009","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1128\/IAI.57.1.235-238.1989","article-title":"Effect of oral immunization with Pseudomonas aeruginosa on the development of specific antibacterial immunity in the lungs","volume":"57","author":"Freihorst","year":"1989","journal-title":"Infection and Immunity"},{"key":"S0022029900030090_ref029","first-page":"331","volume-title":"Recent Advances in Mucosal Immunity","author":"Walker","year":"1982"},{"key":"S0022029900030090_ref019","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-53.5.404"},{"key":"S0022029900030090_ref022","first-page":"315","article-title":"The selective release of lysosomal acid hydrolases from mouse peritoneal macrophages by stimuli of chronic inflammation","volume":"58","author":"Schorlemmer","year":"1977","journal-title":"British Journal of Experimental Pathology"},{"key":"S0022029900030090_ref003","doi-asserted-by":"publisher","DOI":"10.1007\/BF00804826"},{"key":"S0022029900030090_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(86)90086-1"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029900030090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T22:45:02Z","timestamp":1739141102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029900030090\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,11]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1991,11]]}},"alternative-id":["S0022029900030090"],"URL":"https:\/\/doi.org\/10.1017\/s0022029900030090","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,11]]}}}