{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T02:44:20Z","timestamp":1776393860008,"version":"3.51.2"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2005,3,23]],"date-time":"2005-03-23T00:00:00Z","timestamp":1111536000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>This study investigated the protective capacity of the oral administration of fermented milk containing the probiotic strains; <jats:italic>Lactobacillus casei, Lb. delbrueckii<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> and <jats:italic>Streptococcus thermophilus<\/jats:italic>, against enteroinvasive <jats:italic>Escherichia coli<\/jats:italic> infection in a murine (BALB\/c mice) model. Mice were fed for 2, 5 or 7 consecutive days with fermented milk diluted to a concentration of viable <jats:italic>Lb. casei, Lb. delbrueckii<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> and <jats:italic>Strep. thermophilus<\/jats:italic> of 10<jats:sup>7<\/jats:sup> cfu\/ml. Phagocytic activity of peritoneal macrophages and the number of IgA<jats:sup>+<\/jats:sup> cells in small and large intestine were determined at the end of the feeding periods. For the preventive effect against <jats:italic>Esch. coli<\/jats:italic>, animals were fed for 5 days (selected dose). Mice were challenged with an infective dose of enteroinvasive <jats:italic>Esch. coli<\/jats:italic> of 10<jats:sup>8<\/jats:sup> cfu\/mouse. The colonization of liver and spleen and the secretory IgA specific for the pathogen in the intestinal fluid were determined (ELISA test). Results showed that the unspecific immune response enhanced itself after 5 consecutive days of the administration of this fermented milk (increase in the percentage of phagocytosis and number of IgA<jats:sup>+<\/jats:sup> cells in the small intestine). Treated animals showed less <jats:italic>Esch. coli<\/jats:italic> colonization of liver than control mice and a higher secretory anti-<jats:italic>Esch. coli<\/jats:italic> IgA in the intestinal fluids. These results suggest that the protection against enteroinvasive <jats:italic>Esch. coli<\/jats:italic> infection observed for the fermented milk containing probiotic bacteria may be associated with an enhance of the intestinal mucosa immunity.<\/jats:p>","DOI":"10.1017\/s0022029905000750","type":"journal-article","created":{"date-parts":[[2005,4,7]],"date-time":"2005-04-07T11:11:44Z","timestamp":1112872304000},"page":"243-249","source":"Crossref","is-referenced-by-count":34,"title":["Effect of fermented milk containing probiotic bacteria in the prevention of an enteroinvasive <i>Escherichia coli<\/i> infection in mice"],"prefix":"10.1017","volume":"72","author":[{"given":"Marta","family":"Medici","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celso G","family":"Vinderola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Weill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Perdig\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2005,3,23]]},"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029905000750","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T14:52:49Z","timestamp":1554475969000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029905000750\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,23]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["S0022029905000750"],"URL":"https:\/\/doi.org\/10.1017\/s0022029905000750","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,23]]}}}