{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:20:19Z","timestamp":1787505619549,"version":"build-2736575974"},"reference-count":54,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T00:00:00Z","timestamp":1389830400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p>\n                    The aim of the study was to investigate how the use of fresh cheese brines compared with used brines and various combinations of pH and NaCl concentrations affected the survival of\n                    <jats:italic>Listeria monocytogenes.<\/jats:italic>\n                    Cheese brines from five Norwegian small scale cheese producers were analysed and showed great variations in pH (4\u00b754\u20136\u00b701) and NaCl concentrations (14\u00b71\u201326\u00b79\u00a0%). The survival of five strains of\n                    <jats:italic>List. monocytogenes<\/jats:italic>\n                    (two clinical isolates, two food isolates and one animal isolate) in four different cheese brines (three used and one fresh) was investigated. Results showed significant differences in survival both depending on the strains and the brines. Strains of human outbreak listeriosis cases showed greater ability to survive in the brines compared with food isolates and a\n                    <jats:italic>List. monocytogenes<\/jats:italic>\n                    reference strain (1\u20132 log\n                    <jats:sub>10<\/jats:sub>\n                    difference after 200\u00a0d). All strains showed highest survival in the freshly prepared brine compared with the used brines. Molecular typing by multiple locus variable number tandem repeats analysis (MLVA) showed that there were no detectable alterations in the examined variable number tandem repeats of the genome in five strains after 200\u00a0d storage in any of the salt brines. Combined effects of pH (4\u00b75, 5\u00b725 and 6\u00b70) and NaCl (15, 20 and 25\u00a0%) in fresh, filter sterilised brines on the survival of\n                    <jats:italic>List. monocytogenes<\/jats:italic>\n                    were examined and results showed that pathogen populations decreased over time in all brines. Death rates at any given NaCl concentration were highest at low pH (4\u00b75) and death rates at any given pH were highest at low NaCl concentrations (15\u00a0%). In conclusion, the use of used brines reduced the survival of\n                    <jats:italic>List. monocytogenes<\/jats:italic>\n                    and a combination of low pH (4\u00b75) and low salt concentrations (15\u00a0%) decreased the risk of\n                    <jats:italic>List. monocytogenes<\/jats:italic>\n                    survival compared with higher pH (5\u00b725 or 6\u00b70) and higher NaCl concentrations (20 or 25\u00a0%).\n                  <\/jats:p>","DOI":"10.1017\/s0022029913000666","type":"journal-article","created":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T09:17:33Z","timestamp":1389863853000},"page":"113-119","source":"Crossref","is-referenced-by-count":16,"title":["Use of used vs. fresh cheese brines and the effect of pH and salt concentration on the survival of\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1017","volume":"81","author":[{"given":"Bj\u00f8rn C 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