{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:33:04Z","timestamp":1791318784285,"version":"4.3.1"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T00:00:00Z","timestamp":1411948800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>In order to protect peroral \u03b2-galactosidase from being degraded and hydrolyse milk lactose efficiently in the environments of gastrointestinal tract, a double-capsule delivery system composed of enteric-coated capsule and polylactic acid (PLA) nanocapsules (NCs) was developed for encapsulation of \u03b2-galactosidase. \u03b2-galactosidase-loaded PLA NCs in the size range of 100\u2013200\u00a0nm were prepared by a modified w<jats:sub>1<\/jats:sub>\/o\/w<jats:sub>2<\/jats:sub>technique. During the encapsulation process, dichloromethane\/ethyl acetate (1\u00a0:\u00a01, v\/v) as the solvent composition, high-pressure homogenisation (150 bar, 3\u00a0min) as the second emulsification method and polyvinyl alcohol or Poloxamer 188 as a stabiliser in the inner phase could efficiently improve the activity retention of \u03b2-galactosidase (&gt;90%). Subsequently, the prepared NCs were freeze-dried and filled in a hydroxypropyl methylcellulose phthalate (HP55)-coated capsule. In vitro results revealed that the HP55-coated capsule remained intact in the simulated gastric fluid and efficiently protected the nested \u03b2-galactosidase from acidic denaturation. Under the simulated intestinal condition, the enteric coating dissolved rapidly and released the \u03b2-galactosidase-loaded PLA NCs, which exhibited greater stability against enzymatic degradation and higher hydrolysis ratio (\u223c100%) towards milk lactose than the free \u03b2-galactosidase. These results suggest that this double-capsule delivery system represents promising candidate for efficient lactose hydrolysis in the gastrointestinal tract.<\/jats:p>","DOI":"10.1017\/s0022029914000491","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T06:34:17Z","timestamp":1411972457000},"page":"479-484","source":"Crossref","is-referenced-by-count":19,"title":["Enteric-coated capsule containing \u03b2-galactosidase-loaded polylactic acid nanocapsules: enzyme stability and milk lactose hydrolysis under simulated gastrointestinal 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