{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:33:41Z","timestamp":1793680421952,"version":"4.5.1"},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T00:00:00Z","timestamp":1435881600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>This study aimed to describe chronological patterns of changes of various candidate blood components in milk during the acute phase of a mammary immune response in detail. Eight dairy cows were challenged with<jats:italic>Escherichia coli<\/jats:italic>lipopolysaccharide in one udder quarter. Milk from challenged and control quarters and blood samples were taken before, and 1 and 2 h after challenge and then every 15 min until 5 h after challenge. The SCC, serum albumin, immunoglobulin (Ig)G<jats:sub>1<\/jats:sub>, IgG<jats:sub>2<\/jats:sub>, lactate dehydrogenase (LDH), and L-lactate in milk and blood, and \u03b1-lactalbumin in blood were analysed. All selected parameters in milk increased in challenged quarters but did not increase in control quarters. Milk IgG<jats:sub>1<\/jats:sub>, IgG<jats:sub>2<\/jats:sub>, serum albumin, and LDH were already significantly increased at 2 h after challenge whereas a significant increase of SCC was detectable at 2\u00b775 h and L-lactate was increased at 2\u00b725 h after challenge. In blood L-lactate was increased at 3\u00b775 h after challenge, however, other factors in blood did not change significantly within the 5 h of experiment. In conclusion, the increase of blood components in milk during inflammation follows two different patterns: There is a rapid increase for IgG<jats:sub>1<\/jats:sub>, IgG<jats:sub>2<\/jats:sub>, or LDH, before the increase of SCC, and their concentrations reach a plateau within 3 h. On the other hand, SCC and L-lactate show a slower but consistent increase not reaching a plateau within 5 h after LPS challenge. L-lactate increases to higher concentrations in milk than in blood. This clearly shows that the increase of blood components follows different patterns and is therefore a controlled and compound-specific process and not exclusively an unspecific type of leakage.<\/jats:p>","DOI":"10.1017\/s0022029915000345","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T14:57:07Z","timestamp":1435935427000},"page":"322-327","source":"Crossref","is-referenced-by-count":19,"title":["Different chronological patterns of appearance of blood derived milk components during mastitis indicate different mechanisms of transfer from blood into 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