{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:49:54Z","timestamp":1782470994540,"version":"3.54.5"},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>This Regional Research Communication describes the characterisation of ampicillin, penicillin and tetracycline resistance in<jats:italic>Staphylococcus aureus<\/jats:italic>isolated from bovine subclinical mastitis in Minas Gerais State, Brazil. Ninety<jats:italic>S. aureus<\/jats:italic>isolates from bovine mastitis exhibiting phenotypic resistance to ampicillin, penicillin and\/or tetracycline were selected for this study. The minimum inhibitory concentration (MIC) of each antibiotic was determined using the E-Test<jats:sup>\u00ae<\/jats:sup>and the production of beta-lactamase was determined by cefinase disks. The resistance genes<jats:italic>blaZ, tet<\/jats:italic>(K),<jats:italic>tet<\/jats:italic>(L),<jats:italic>tet<\/jats:italic>(M), and<jats:italic>tet<\/jats:italic>(O) were investigated by PCR in all of the isolates. The MIC results classified 77, 83 and 71% of the isolates as resistant to ampicillin, penicillin and tetracycline, respectively. The MIC<jats:sub>50<\/jats:sub>and MIC<jats:sub>90<\/jats:sub>were, respectively, 1 and 2 \u00b5g\/ml for ampicillin, 0\u00b75 and 1 \u00b5g\/ml for penicillin and 32 and 64 \u00b5g\/ml for tetracycline. Eighty-six per cent of beta-lactamase producing isolates were detected. Of the 90 isolates investigated, 97% amplified<jats:italic>blaZ<\/jats:italic>, 84% amplified<jats:italic>tet<\/jats:italic>(K), 9% amplified<jats:italic>tet<\/jats:italic>(L), 2% amplified<jats:italic>tet<\/jats:italic>(M) and 1% amplified<jats:italic>tet<\/jats:italic>(O). Seventy-nine isolates (88%) showed<jats:italic>blaZ<\/jats:italic>together with at least one<jats:italic>tet<\/jats:italic>gene.<jats:italic>S. aureus<\/jats:italic>isolates showed high MIC<jats:sub>50<\/jats:sub>and MIC<jats:sub>90<\/jats:sub>values for the three antimicrobials. The<jats:italic>bla<\/jats:italic>Z and<jats:italic>tet<\/jats:italic>(K) genes were widespread in the herds studied, and most of the isolates harboured<jats:italic>bla<\/jats:italic>Z and<jats:italic>tet<\/jats:italic>(K) concomitantly.<\/jats:p>","DOI":"10.1017\/s0022029917000061","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T12:19:26Z","timestamp":1489493966000},"page":"202-205","source":"Crossref","is-referenced-by-count":30,"title":["Characterisation of penicillin and tetracycline resistance in<i>Staphylococcus aureus<\/i>isolated from bovine milk samples in Minas Gerais, Brazil"],"prefix":"10.1017","volume":"84","author":[{"given":"Caroline L","family":"Martini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla C","family":"Lange","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria AVP","family":"Brito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o B","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia C","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana K","family":"Vaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"S0022029917000061_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880200715X"},{"key":"S0022029917000061_ref7","doi-asserted-by":"publisher","DOI":"10.1024\/0036-7281\/a000471"},{"key":"S0022029917000061_ref8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.763"},{"key":"S0022029917000061_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.08.030"},{"key":"S0022029917000061_ref5","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(04)73366-4"},{"key":"S0022029917000061_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.006"},{"key":"S0022029917000061_ref1","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard","year":"2013"},{"key":"S0022029917000061_ref9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1186\/BF03547026","article-title":"Ocorrence of the blaZ in penicillin resistant Staphylococcus aureus isolated from bovine mastitis in Denmark","volume":"40","author":"Vesterholm-Nielsen","year":"1999","journal-title":"Acta Veterinaria Scandinavica"},{"key":"S0022029917000061_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.12.015"},{"key":"S0022029917000061_ref6","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029915000242"},{"key":"S0022029917000061_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.01.024"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029917000061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T04:10:12Z","timestamp":1658808612000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029917000061\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,14]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["S0022029917000061"],"URL":"https:\/\/doi.org\/10.1017\/s0022029917000061","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,14]]}}}