{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T05:14:42Z","timestamp":1793855682041,"version":"4.6.1"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T00:00:00Z","timestamp":1530489600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>Evaluation of eleven candidate probiotic<jats:italic>Lactobacillus<\/jats:italic>strains isolated from human milk showed that some of the strains were well endowed with desirable cell surface and attachment attributes. The cell surface properties (hydrophobicity, auto-aggregation, attachment to collagen and HT-29 monolayer) of probiotic<jats:italic>Lactobacillus<\/jats:italic>species of human milk origin were compared with reference probiotic\/ non-probiotic species and pathogenic strains. The bacterial adhesion to hydrocarbons (BATH) was determined using three aliphatic (Chloroform, n-Hexane and n-Octane) and two aromatic (Toluene and Xylene) solvents. Maximum affinity of<jats:italic>Lactobacillus<\/jats:italic>strains towards chloroform and toluene indicated the presence of low electron acceptor\/ acidic surface components on cell surface of most of the strains. The highest value of per cent hydrophobicity was recorded with chloroform in HM1 (<jats:italic>L. casei<\/jats:italic>) (97\u00b710 \u00b1 3\u00b735%) and LGG (98\u00b792 \u00b1 1\u00b724%). A moderate auto-aggregation attribute was observed in all of our<jats:italic>Lactobacillus<\/jats:italic>isolates. Only HM10, HM12 and HM13 exhibited comparatively enhanced precipitation rate after 7 h of incubation period. The adhesion potential to collagen matrix was highest in LGG (26\u00b794 \u00b1 5\u00b783%), followed by HM1 (11\u00b707 \u00b1 3\u00b754%) and HM9 (10\u00b785 \u00b1 1\u00b774%) whereas, on HT-29 cells, HM8 (14\u00b799 \u00b1 3\u00b761%), HM3 (13\u00b773 \u00b1 1\u00b714%) and HM1 (11\u00b721 \u00b1 3\u00b718%) could adhere effectively. In this manner, we noticed that although the cell surface properties and adhesion prospective of probiotic bacteria were strain dependent, five of our isolates<jats:italic>viz<\/jats:italic>. HM1, HM3, HM8, HM9 and HM10 exhibited promising cell surface properties, which could be further targeted as indigenous probiotic.<\/jats:p>","DOI":"10.1017\/s0022029918000432","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T06:37:43Z","timestamp":1530513463000},"page":"347-354","source":"Crossref","is-referenced-by-count":69,"title":["Screening of cell surface properties of potential probiotic lactobacilli isolated from human milk"],"prefix":"10.1017","volume":"85","author":[{"given":"Namita","family":"Rokana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brij Pal","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishchal","family":"Thakur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chetan","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohini Devidas","family":"Gulhane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh","family":"Panwar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2018,7,2]]},"reference":[{"key":"S0022029918000432_ref30","first-page":"194","article-title":"Effect of fatty acids on hydrophobicity of the cell membrane of Lactobacillus species","volume":"3","author":"Shahbazi","year":"2016","journal-title":"Applied Food Biotechnology"},{"key":"S0022029918000432_ref24","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12434"},{"key":"S0022029918000432_ref20","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13153"},{"key":"S0022029918000432_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12238"},{"key":"S0022029918000432_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.08.021"},{"key":"S0022029918000432_ref8","doi-asserted-by":"crossref","first-page":"664","DOI":"10.4103\/0971-5916.90992","article-title":"Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells","volume":"134","author":"Duary","year":"2011","journal-title":"The Indian Journal of Medical Research"},{"key":"S0022029918000432_ref4","doi-asserted-by":"publisher","DOI":"10.1038\/35014000"},{"key":"S0022029918000432_ref32","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-017-0682-0"},{"key":"S0022029918000432_ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-017-9270-1"},{"key":"S0022029918000432_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3991(03)00044-5"},{"key":"S0022029918000432_ref12","doi-asserted-by":"publisher","DOI":"10.3390\/biology3010178"},{"key":"S0022029918000432_ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-016-0591-7"},{"key":"S0022029918000432_ref21","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6110-z"},{"key":"S0022029918000432_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2014.01.010"},{"key":"S0022029918000432_ref27","doi-asserted-by":"publisher","DOI":"10.1021\/la048372z"},{"key":"S0022029918000432_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2013.05.003"},{"key":"S0022029918000432_ref19","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/538171"},{"key":"S0022029918000432_ref37","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.6b00499"},{"key":"S0022029918000432_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.03.046"},{"key":"S0022029918000432_ref29","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/237921"},{"key":"S0022029918000432_ref3","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.066"},{"key":"S0022029918000432_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00399-9"},{"key":"S0022029918000432_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-014-0426-1"},{"key":"S0022029918000432_ref14","first-page":"84","article-title":"Aggregation and adhesion abilities of 18 lactic acid bacteria strains isolated from traditional fermented food","volume":"3","author":"Li","year":"2015","journal-title":"International Journal of Agriculture Policy and Research"},{"key":"S0022029918000432_ref41","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2009.02684.x"},{"key":"S0022029918000432_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.01.011"},{"key":"S0022029918000432_ref13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.01915.x"},{"key":"S0022029918000432_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2014.07.002"},{"key":"S0022029918000432_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2013.06.001"},{"key":"S0022029918000432_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2016.03.016"},{"key":"S0022029918000432_ref35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038034"},{"key":"S0022029918000432_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2010.01.004"},{"key":"S0022029918000432_ref26","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1128\/IAI.42.2.831-834.1983","article-title":"Cell surface hydrophobicity of dental plaque microorganisms in situ","volume":"42","author":"Rosenberg","year":"1983","journal-title":"Infection and Immunity"},{"key":"S0022029918000432_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.02.026"},{"key":"S0022029918000432_ref17","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00926-13"},{"key":"S0022029918000432_ref23","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/286390"},{"key":"S0022029918000432_ref33","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12642"},{"key":"S0022029918000432_ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2007.00294.x"},{"key":"S0022029918000432_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/0927-7765(95)01225-8"},{"key":"S0022029918000432_ref40","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.12695"},{"key":"S0022029918000432_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126387"},{"key":"S0022029918000432_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00224-4"}],"container-title":["Journal of Dairy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022029918000432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T21:43:11Z","timestamp":1604526191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022029918000432\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,2]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["S0022029918000432"],"URL":"https:\/\/doi.org\/10.1017\/s0022029918000432","relation":{},"ISSN":["0022-0299","1469-7629"],"issn-type":[{"value":"0022-0299","type":"print"},{"value":"1469-7629","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,2]]}}}