{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:16:36Z","timestamp":1789157796588,"version":"build-2803163510"},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"unspecified","delay-in-days":37,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Dairy Research"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Identification of the most proteolytic<jats:italic>Pseudomonas<\/jats:italic>strains that can produce heat-resistant proteases and contribute to the Ultra High Temperature (UHT) milk destabilization is of great interest. In the present study, among the 146<jats:italic>Pseudomonas<\/jats:italic>isolates that encoded the<jats:italic>apr<\/jats:italic>X gene, five isolates with the highest proteolytic activity were selected and identified based on 16S rRNA,<jats:italic>rpoD<\/jats:italic>and<jats:italic>gyrB<\/jats:italic>gene sequences data. The identification results were confirmed by phylogenetic analysis based on multilocus sequence analysis and identified the representative isolates as<jats:italic>P. jessenii<\/jats:italic>(two isolates) and<jats:italic>P. gessardii<\/jats:italic>(three isolates). Casein zymography demonstrated the ability of these species to produce heat-resistant enzymes, AprX, with molecular mass of about 48 kDa during storage at 7\u00b0 C for 72 h. In sterilized milk samples, the residual activity of AprX caused a considerable enhancement in the degree of protein hydrolysis, non-protein nitrogen and non-casein nitrogen contents of the samples during a two-month storage. This enhancement was slightly higher in samples containing enzyme produced by<jats:italic>P. jessenii<\/jats:italic>compared to<jats:italic>P. gessardii<\/jats:italic>ones, resulting in earlier onset of sterilized milk destabilization. Hence, this study revealed that<jats:italic>P. jessenii<\/jats:italic>and<jats:italic>P. gessardii<\/jats:italic>can play a considerable role in deterioration of Iranian commercial long-life milk.<\/jats:p>","DOI":"10.1017\/s0022029920000709","type":"journal-article","created":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T09:24:17Z","timestamp":1599470657000},"page":"368-374","source":"Crossref","is-referenced-by-count":16,"title":["Identification of<i>Pseudomonas jessenii<\/i>and<i>Pseudomonas gessardii<\/i>as the most proteolytic<i>Pseudomonas<\/i>isolates in Iranian raw milk and their impact on stability of sterilized milk during 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